Experiment: BHIS with Lithocholic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4064 and BT4065 are separated by 125 nucleotides BT4065 and BT4066 overlap by 4 nucleotides BT4066 and BT4067 overlap by 10 nucleotides
BT4064: BT4064 - NADH dehydrogenase I, chain H (NCBI ptt file), at 5,297,477 to 5,298,553
BT4064
BT4065: BT4065 - NADH dehydrogenase I, chain D (NCBI ptt file), at 5,298,679 to 5,300,295
BT4065
BT4066: BT4066 - NADH dehydrogenase I, chain B (NCBI ptt file), at 5,300,292 to 5,300,885
BT4066
BT4067: BT4067 - NADH dehydrogenase I, chain A (NCBI ptt file), at 5,300,876 to 5,301,226
BT4067
Position (kb)
5298
5299
5300
5301 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5297.772 kb on + strand, within BT4064 at 5297.784 kb on + strand, within BT4064 at 5297.785 kb on - strand, within BT4064 at 5297.818 kb on + strand, within BT4064 at 5297.901 kb on + strand, within BT4064 at 5297.929 kb on + strand, within BT4064 at 5297.944 kb on + strand, within BT4064 at 5298.043 kb on - strand, within BT4064 at 5298.098 kb on - strand, within BT4064 at 5298.100 kb on + strand, within BT4064 at 5298.121 kb on + strand, within BT4064 at 5298.122 kb on - strand, within BT4064 at 5298.172 kb on + strand, within BT4064 at 5298.196 kb on + strand, within BT4064 at 5298.261 kb on + strand, within BT4064 at 5298.262 kb on - strand, within BT4064 at 5298.318 kb on - strand, within BT4064 at 5298.337 kb on + strand, within BT4064 at 5298.430 kb on + strand, within BT4064 at 5298.435 kb on + strand, within BT4064 at 5298.435 kb on + strand, within BT4064 at 5298.436 kb on - strand, within BT4064 at 5298.436 kb on - strand, within BT4064 at 5298.438 kb on - strand, within BT4064 at 5298.465 kb on - strand at 5298.475 kb on + strand at 5298.553 kb on + strand at 5298.554 kb on - strand at 5298.563 kb on - strand at 5298.587 kb on + strand at 5298.590 kb on + strand at 5298.612 kb on + strand at 5298.651 kb on + strand at 5298.655 kb on + strand at 5298.658 kb on + strand at 5298.871 kb on + strand, within BT4065 at 5298.884 kb on + strand, within BT4065 at 5298.996 kb on + strand, within BT4065 at 5298.996 kb on + strand, within BT4065 at 5298.996 kb on + strand, within BT4065 at 5298.996 kb on + strand, within BT4065 at 5299.007 kb on + strand, within BT4065 at 5299.064 kb on + strand, within BT4065 at 5299.066 kb on + strand, within BT4065 at 5299.090 kb on + strand, within BT4065 at 5299.162 kb on + strand, within BT4065 at 5299.182 kb on + strand, within BT4065 at 5299.213 kb on + strand, within BT4065 at 5299.236 kb on + strand, within BT4065 at 5299.245 kb on + strand, within BT4065 at 5299.245 kb on + strand, within BT4065 at 5299.247 kb on + strand, within BT4065 at 5299.254 kb on + strand, within BT4065 at 5299.300 kb on + strand, within BT4065 at 5299.319 kb on + strand, within BT4065 at 5299.483 kb on - strand, within BT4065 at 5299.490 kb on + strand, within BT4065 at 5299.539 kb on + strand, within BT4065 at 5299.540 kb on - strand, within BT4065 at 5299.614 kb on + strand, within BT4065 at 5299.614 kb on + strand, within BT4065 at 5299.650 kb on + strand, within BT4065 at 5299.715 kb on - strand, within BT4065 at 5299.757 kb on + strand, within BT4065 at 5299.757 kb on + strand, within BT4065 at 5299.857 kb on + strand, within BT4065 at 5299.857 kb on + strand, within BT4065 at 5299.887 kb on + strand at 5299.887 kb on + strand, within BT4065 at 5299.894 kb on - strand, within BT4065 at 5299.924 kb on + strand, within BT4065 at 5299.928 kb on + strand, within BT4065 at 5299.970 kb on + strand, within BT4065 at 5299.971 kb on - strand, within BT4065 at 5299.973 kb on + strand, within BT4065 at 5300.237 kb on - strand at 5300.251 kb on + strand at 5300.272 kb on - strand at 5300.354 kb on + strand, within BT4066 at 5300.365 kb on + strand, within BT4066 at 5300.386 kb on + strand, within BT4066 at 5300.386 kb on + strand, within BT4066 at 5300.386 kb on + strand, within BT4066 at 5300.420 kb on + strand, within BT4066 at 5300.420 kb on + strand, within BT4066 at 5300.422 kb on + strand, within BT4066 at 5300.424 kb on + strand, within BT4066 at 5300.424 kb on + strand, within BT4066 at 5300.444 kb on + strand, within BT4066 at 5300.444 kb on + strand, within BT4066 at 5300.467 kb on + strand, within BT4066 at 5300.521 kb on + strand, within BT4066 at 5300.545 kb on + strand, within BT4066 at 5300.603 kb on + strand, within BT4066 at 5300.603 kb on + strand, within BT4066 at 5300.714 kb on + strand, within BT4066 at 5300.910 kb on + strand at 5300.935 kb on + strand, within BT4067 at 5300.950 kb on + strand, within BT4067 at 5300.950 kb on + strand, within BT4067 at 5300.983 kb on + strand, within BT4067 at 5300.983 kb on + strand, within BT4067 at 5301.059 kb on + strand, within BT4067 at 5301.097 kb on + strand, within BT4067 at 5301.243 kb on + strand at 5301.255 kb on + strand at 5301.257 kb on + strand at 5301.273 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Lithocholic 0.4 mg/ml remove 5,297,772 + BT4064 0.27 -2.3 5,297,784 + BT4064 0.29 -1.5 5,297,785 - BT4064 0.29 -1.3 5,297,818 + BT4064 0.32 -0.3 5,297,901 + BT4064 0.39 -2.6 5,297,929 + BT4064 0.42 -0.0 5,297,944 + BT4064 0.43 -0.7 5,298,043 - BT4064 0.53 -0.3 5,298,098 - BT4064 0.58 -1.3 5,298,100 + BT4064 0.58 -3.7 5,298,121 + BT4064 0.60 +0.3 5,298,122 - BT4064 0.60 +2.6 5,298,172 + BT4064 0.65 -0.3 5,298,196 + BT4064 0.67 -0.3 5,298,261 + BT4064 0.73 -1.0 5,298,262 - BT4064 0.73 -1.0 5,298,318 - BT4064 0.78 -0.3 5,298,337 + BT4064 0.80 -0.3 5,298,430 + BT4064 0.88 -0.3 5,298,435 + BT4064 0.89 -0.3 5,298,435 + BT4064 0.89 -2.6 5,298,436 - BT4064 0.89 -1.1 5,298,436 - BT4064 0.89 -1.4 5,298,438 - BT4064 0.89 -1.0 5,298,465 - +0.3 5,298,475 + +1.7 5,298,553 + -1.9 5,298,554 - -2.1 5,298,563 - -1.4 5,298,587 + -3.3 5,298,590 + -3.7 5,298,612 + -2.0 5,298,651 + -0.7 5,298,655 + +0.5 5,298,658 + -1.5 5,298,871 + BT4065 0.12 -0.8 5,298,884 + BT4065 0.13 +0.9 5,298,996 + BT4065 0.20 -0.1 5,298,996 + BT4065 0.20 -0.6 5,298,996 + BT4065 0.20 +0.3 5,298,996 + BT4065 0.20 -2.2 5,299,007 + BT4065 0.20 -0.3 5,299,064 + BT4065 0.24 -0.6 5,299,066 + BT4065 0.24 +0.6 5,299,090 + BT4065 0.25 -0.3 5,299,162 + BT4065 0.30 -1.0 5,299,182 + BT4065 0.31 -0.1 5,299,213 + BT4065 0.33 +0.3 5,299,236 + BT4065 0.34 +0.6 5,299,245 + BT4065 0.35 -2.0 5,299,245 + BT4065 0.35 -2.3 5,299,247 + BT4065 0.35 -0.3 5,299,254 + BT4065 0.36 +0.5 5,299,300 + BT4065 0.38 -0.0 5,299,319 + BT4065 0.40 -1.0 5,299,483 - BT4065 0.50 +1.3 5,299,490 + BT4065 0.50 -0.0 5,299,539 + BT4065 0.53 -0.3 5,299,540 - BT4065 0.53 +0.5 5,299,614 + BT4065 0.58 -2.7 5,299,614 + BT4065 0.58 -3.3 5,299,650 + BT4065 0.60 +0.3 5,299,715 - BT4065 0.64 -1.7 5,299,757 + BT4065 0.67 -0.7 5,299,757 + BT4065 0.67 -0.1 5,299,857 + BT4065 0.73 -2.0 5,299,857 + BT4065 0.73 -2.9 5,299,887 + -1.3 5,299,887 + BT4065 0.75 +0.6 5,299,894 - BT4065 0.75 -1.0 5,299,924 + BT4065 0.77 -1.1 5,299,928 + BT4065 0.77 -1.6 5,299,970 + BT4065 0.80 -1.5 5,299,971 - BT4065 0.80 -0.7 5,299,973 + BT4065 0.80 -2.5 5,300,237 - +0.3 5,300,251 + -1.4 5,300,272 - -1.3 5,300,354 + BT4066 0.10 -1.3 5,300,365 + BT4066 0.12 -0.8 5,300,386 + BT4066 0.16 +1.0 5,300,386 + BT4066 0.16 -3.2 5,300,386 + BT4066 0.16 -3.3 5,300,420 + BT4066 0.22 -2.2 5,300,420 + BT4066 0.22 +0.5 5,300,422 + BT4066 0.22 +0.1 5,300,424 + BT4066 0.22 +0.9 5,300,424 + BT4066 0.22 +0.1 5,300,444 + BT4066 0.26 -1.5 5,300,444 + BT4066 0.26 -0.0 5,300,467 + BT4066 0.29 -1.9 5,300,521 + BT4066 0.39 -2.6 5,300,545 + BT4066 0.43 -1.5 5,300,603 + BT4066 0.52 +0.2 5,300,603 + BT4066 0.52 -1.3 5,300,714 + BT4066 0.71 -1.6 5,300,910 + -0.6 5,300,935 + BT4067 0.17 -1.5 5,300,950 + BT4067 0.21 -1.7 5,300,950 + BT4067 0.21 -2.0 5,300,983 + BT4067 0.30 -0.3 5,300,983 + BT4067 0.30 -0.7 5,301,059 + BT4067 0.52 -2.5 5,301,097 + BT4067 0.63 -0.7 5,301,243 + -1.3 5,301,255 + -1.6 5,301,257 + -0.7 5,301,273 + -0.4
Or see this region's nucleotide sequence