Experiment: BHIS with Lithocholic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3184 and BT3185 are separated by 107 nucleotides BT3185 and BT3186 are separated by 11 nucleotides
BT3184: BT3184 - L-aspartate oxidase (NCBI ptt file), at 4,072,734 to 4,074,305
BT3184
BT3185: BT3185 - hypothetical protein (NCBI ptt file), at 4,074,413 to 4,074,808
BT3185
BT3186: BT3186 - dihydrolipoamide dehydrogenase (NCBI ptt file), at 4,074,820 to 4,076,163
BT3186
Position (kb)
4074
4075 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4073.468 kb on - strand, within BT3184 at 4073.505 kb on - strand, within BT3184 at 4073.565 kb on + strand, within BT3184 at 4073.566 kb on + strand, within BT3184 at 4073.566 kb on + strand, within BT3184 at 4073.612 kb on + strand, within BT3184 at 4073.627 kb on - strand, within BT3184 at 4073.691 kb on + strand, within BT3184 at 4073.709 kb on - strand, within BT3184 at 4073.728 kb on + strand, within BT3184 at 4073.729 kb on - strand, within BT3184 at 4073.729 kb on - strand, within BT3184 at 4073.732 kb on + strand, within BT3184 at 4073.732 kb on + strand, within BT3184 at 4073.732 kb on + strand, within BT3184 at 4073.732 kb on + strand, within BT3184 at 4073.732 kb on + strand, within BT3184 at 4073.732 kb on + strand, within BT3184 at 4073.764 kb on + strand, within BT3184 at 4073.833 kb on - strand, within BT3184 at 4073.924 kb on + strand, within BT3184 at 4073.925 kb on - strand, within BT3184 at 4073.931 kb on - strand, within BT3184 at 4074.131 kb on + strand, within BT3184 at 4074.182 kb on + strand at 4074.182 kb on + strand at 4074.183 kb on - strand at 4074.183 kb on - strand at 4074.213 kb on - strand at 4074.301 kb on - strand at 4074.340 kb on + strand at 4074.477 kb on + strand, within BT3185 at 4074.507 kb on - strand, within BT3185 at 4074.546 kb on + strand, within BT3185 at 4074.562 kb on + strand, within BT3185 at 4074.563 kb on - strand, within BT3185 at 4074.575 kb on + strand, within BT3185 at 4074.643 kb on + strand, within BT3185 at 4074.643 kb on + strand, within BT3185 at 4074.643 kb on + strand, within BT3185 at 4074.662 kb on - strand, within BT3185 at 4074.710 kb on + strand, within BT3185 at 4074.814 kb on - strand at 4074.819 kb on + strand at 4074.827 kb on - strand at 4074.864 kb on + strand at 4074.864 kb on + strand at 4074.864 kb on + strand at 4074.865 kb on - strand at 4074.865 kb on - strand at 4074.865 kb on - strand at 4074.902 kb on + strand at 4074.921 kb on + strand at 4074.992 kb on - strand, within BT3186 at 4075.098 kb on - strand, within BT3186 at 4075.164 kb on - strand, within BT3186 at 4075.167 kb on + strand, within BT3186 at 4075.168 kb on - strand, within BT3186 at 4075.199 kb on - strand, within BT3186 at 4075.335 kb on - strand, within BT3186 at 4075.469 kb on - strand, within BT3186 at 4075.504 kb on - strand, within BT3186 at 4075.517 kb on + strand, within BT3186 at 4075.518 kb on - strand, within BT3186 at 4075.549 kb on + strand, within BT3186 at 4075.787 kb on + strand, within BT3186 at 4075.787 kb on + strand, within BT3186 at 4075.794 kb on + strand, within BT3186
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Lithocholic 0.4 mg/ml remove 4,073,468 - BT3184 0.47 -1.7 4,073,505 - BT3184 0.49 -1.9 4,073,565 + BT3184 0.53 -1.8 4,073,566 + BT3184 0.53 -3.3 4,073,566 + BT3184 0.53 -1.2 4,073,612 + BT3184 0.56 -1.3 4,073,627 - BT3184 0.57 -2.3 4,073,691 + BT3184 0.61 -2.6 4,073,709 - BT3184 0.62 -1.9 4,073,728 + BT3184 0.63 -3.0 4,073,729 - BT3184 0.63 -1.0 4,073,729 - BT3184 0.63 -4.2 4,073,732 + BT3184 0.63 -1.5 4,073,732 + BT3184 0.63 -0.7 4,073,732 + BT3184 0.63 -3.6 4,073,732 + BT3184 0.63 -4.4 4,073,732 + BT3184 0.63 -2.5 4,073,732 + BT3184 0.63 -4.0 4,073,764 + BT3184 0.66 -1.6 4,073,833 - BT3184 0.70 -1.5 4,073,924 + BT3184 0.76 -2.0 4,073,925 - BT3184 0.76 -2.4 4,073,931 - BT3184 0.76 -2.2 4,074,131 + BT3184 0.89 -1.2 4,074,182 + -0.4 4,074,182 + -2.3 4,074,183 - -2.8 4,074,183 - -2.3 4,074,213 - -2.2 4,074,301 - -1.6 4,074,340 + -0.5 4,074,477 + BT3185 0.16 -0.8 4,074,507 - BT3185 0.24 -1.0 4,074,546 + BT3185 0.34 +0.6 4,074,562 + BT3185 0.38 -0.8 4,074,563 - BT3185 0.38 -0.8 4,074,575 + BT3185 0.41 -0.4 4,074,643 + BT3185 0.58 -0.1 4,074,643 + BT3185 0.58 +0.2 4,074,643 + BT3185 0.58 -0.8 4,074,662 - BT3185 0.63 +1.0 4,074,710 + BT3185 0.75 +0.5 4,074,814 - -0.9 4,074,819 + -0.2 4,074,827 - -1.2 4,074,864 + -2.3 4,074,864 + -0.5 4,074,864 + -0.5 4,074,865 - -0.6 4,074,865 - -2.3 4,074,865 - -1.6 4,074,902 + -3.2 4,074,921 + +0.5 4,074,992 - BT3186 0.13 -0.8 4,075,098 - BT3186 0.21 -2.7 4,075,164 - BT3186 0.26 +1.0 4,075,167 + BT3186 0.26 +0.3 4,075,168 - BT3186 0.26 -1.5 4,075,199 - BT3186 0.28 -1.4 4,075,335 - BT3186 0.38 +0.5 4,075,469 - BT3186 0.48 -0.8 4,075,504 - BT3186 0.51 -0.0 4,075,517 + BT3186 0.52 +0.3 4,075,518 - BT3186 0.52 -2.2 4,075,549 + BT3186 0.54 -0.5 4,075,787 + BT3186 0.72 -1.5 4,075,787 + BT3186 0.72 -1.5 4,075,794 + BT3186 0.72 -0.1
Or see this region's nucleotide sequence