Experiment: BHIS with Lithocholic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0601 and BT0602 are separated by 19 nucleotides BT0602 and BT0603 are separated by 143 nucleotides
BT0601: BT0601 - UDP-N-acetylglucosamine 2-epimerase (NCBI ptt file), at 741,713 to 742,924
BT0601
BT0602: BT0602 - UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI ptt file), at 742,944 to 744,167
BT0602
BT0603: BT0603 - conserved hypothetical protein (NCBI ptt file), at 744,311 to 745,423
BT0603
Position (kb) 742
743
744
745 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 742.098 kb on - strand, within BT0601 at 742.217 kb on + strand, within BT0601 at 742.250 kb on + strand, within BT0601 at 742.250 kb on + strand, within BT0601 at 742.253 kb on - strand, within BT0601 at 742.258 kb on - strand, within BT0601 at 742.262 kb on - strand, within BT0601 at 742.309 kb on - strand, within BT0601 at 742.421 kb on - strand, within BT0601 at 742.464 kb on + strand, within BT0601 at 742.464 kb on + strand, within BT0601 at 742.465 kb on - strand, within BT0601 at 742.471 kb on + strand, within BT0601 at 742.597 kb on + strand, within BT0601 at 742.598 kb on - strand, within BT0601 at 742.665 kb on + strand, within BT0601 at 742.666 kb on - strand, within BT0601 at 742.675 kb on + strand, within BT0601 at 742.733 kb on + strand, within BT0601 at 742.877 kb on + strand at 743.051 kb on + strand at 743.086 kb on + strand, within BT0602 at 743.139 kb on - strand, within BT0602 at 743.162 kb on - strand, within BT0602 at 743.162 kb on - strand, within BT0602 at 743.163 kb on + strand, within BT0602 at 743.181 kb on - strand, within BT0602 at 743.275 kb on + strand, within BT0602 at 743.276 kb on - strand, within BT0602 at 743.366 kb on - strand, within BT0602 at 743.481 kb on + strand, within BT0602 at 743.482 kb on - strand, within BT0602 at 743.573 kb on + strand, within BT0602 at 743.622 kb on - strand, within BT0602 at 743.843 kb on + strand, within BT0602 at 743.954 kb on - strand, within BT0602 at 743.973 kb on + strand, within BT0602 at 744.166 kb on - strand at 744.283 kb on - strand at 744.335 kb on + strand at 744.337 kb on + strand at 744.338 kb on - strand at 744.338 kb on - strand at 744.370 kb on - strand at 744.563 kb on - strand, within BT0603 at 744.631 kb on + strand, within BT0603 at 744.789 kb on + strand, within BT0603 at 744.889 kb on - strand, within BT0603 at 744.943 kb on + strand, within BT0603
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Lithocholic 0.4 mg/ml remove 742,098 - BT0601 0.32 +1.2 742,217 + BT0601 0.42 -2.3 742,250 + BT0601 0.44 -2.6 742,250 + BT0601 0.44 -0.1 742,253 - BT0601 0.45 -0.8 742,258 - BT0601 0.45 -2.7 742,262 - BT0601 0.45 -1.9 742,309 - BT0601 0.49 +0.2 742,421 - BT0601 0.58 +0.2 742,464 + BT0601 0.62 -0.1 742,464 + BT0601 0.62 -0.9 742,465 - BT0601 0.62 -0.6 742,471 + BT0601 0.63 -1.1 742,597 + BT0601 0.73 -0.4 742,598 - BT0601 0.73 -2.2 742,665 + BT0601 0.79 -0.4 742,666 - BT0601 0.79 -1.4 742,675 + BT0601 0.79 -1.9 742,733 + BT0601 0.84 +0.2 742,877 + -0.4 743,051 + +1.2 743,086 + BT0602 0.12 -1.4 743,139 - BT0602 0.16 +0.4 743,162 - BT0602 0.18 -1.4 743,162 - BT0602 0.18 -1.2 743,163 + BT0602 0.18 -1.1 743,181 - BT0602 0.19 -1.4 743,275 + BT0602 0.27 -1.1 743,276 - BT0602 0.27 -2.4 743,366 - BT0602 0.34 -1.4 743,481 + BT0602 0.44 -0.9 743,482 - BT0602 0.44 +0.2 743,573 + BT0602 0.51 -1.9 743,622 - BT0602 0.55 -1.8 743,843 + BT0602 0.73 +0.9 743,954 - BT0602 0.83 -2.5 743,973 + BT0602 0.84 +0.2 744,166 - -2.1 744,283 - +1.5 744,335 + +0.2 744,337 + -2.8 744,338 - -0.1 744,338 - +0.2 744,370 - -1.4 744,563 - BT0603 0.23 -0.6 744,631 + BT0603 0.29 +2.2 744,789 + BT0603 0.43 -1.1 744,889 - BT0603 0.52 -0.1 744,943 + BT0603 0.57 -0.4
Or see this region's nucleotide sequence