Experiment: BHIS with Lithocholic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0426 and BT0427 overlap by 7 nucleotides BT0427 and BT0428 are separated by 96 nucleotides BT0428 and BT0429 are separated by 11 nucleotides BT0429 and BT0430 are separated by 3 nucleotides
BT0426: BT0426 - conserved hypothetical protein (NCBI ptt file), at 513,117 to 513,887
BT0426
BT0427: BT0427 - xanthine phosphoribosyltransferase (NCBI ptt file), at 513,881 to 514,453
BT0427
BT0428: BT0428 - phenylacetate-coenzyme A ligase (NCBI ptt file), at 514,550 to 515,857
BT0428
BT0429: BT0429 - indolepyruvate oxidoreductase subunit iorB (NCBI ptt file), at 515,869 to 516,453
BT0429
BT0430: BT0430 - indolepyruvate oxidoreductase subunit iorA (NCBI ptt file), at 516,457 to 518,049
BT0430
Position (kb) 514
515
516 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 513.564 kb on - strand, within BT0426 at 513.801 kb on + strand, within BT0426 at 513.802 kb on - strand, within BT0426 at 513.832 kb on - strand at 513.836 kb on + strand at 513.837 kb on - strand at 513.901 kb on + strand at 514.144 kb on - strand, within BT0427 at 514.245 kb on + strand, within BT0427 at 514.246 kb on - strand, within BT0427 at 514.283 kb on + strand, within BT0427 at 514.284 kb on - strand, within BT0427 at 514.457 kb on - strand at 514.480 kb on + strand at 514.481 kb on - strand at 514.507 kb on - strand at 514.528 kb on - strand at 514.672 kb on + strand at 514.673 kb on - strand at 514.702 kb on - strand, within BT0428 at 514.775 kb on - strand, within BT0428 at 514.783 kb on - strand, within BT0428 at 514.783 kb on - strand, within BT0428 at 514.859 kb on - strand, within BT0428 at 514.935 kb on - strand, within BT0428 at 514.994 kb on - strand, within BT0428 at 515.067 kb on + strand, within BT0428 at 515.153 kb on - strand, within BT0428 at 515.156 kb on - strand, within BT0428 at 515.158 kb on - strand, within BT0428 at 515.158 kb on - strand, within BT0428 at 515.261 kb on - strand, within BT0428 at 515.298 kb on + strand, within BT0428 at 515.315 kb on + strand, within BT0428 at 515.330 kb on - strand, within BT0428 at 515.363 kb on - strand, within BT0428 at 515.558 kb on - strand, within BT0428 at 515.594 kb on + strand, within BT0428 at 515.702 kb on - strand, within BT0428 at 515.702 kb on - strand, within BT0428 at 515.705 kb on - strand, within BT0428 at 515.746 kb on - strand at 515.866 kb on + strand at 515.867 kb on - strand at 515.870 kb on + strand at 515.917 kb on - strand at 516.091 kb on - strand, within BT0429 at 516.118 kb on + strand, within BT0429 at 516.207 kb on - strand, within BT0429 at 516.360 kb on - strand, within BT0429 at 516.391 kb on - strand, within BT0429 at 516.437 kb on - strand at 516.439 kb on - strand at 516.463 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Lithocholic 0.4 mg/ml remove 513,564 - BT0426 0.58 -1.1 513,801 + BT0426 0.89 -1.7 513,802 - BT0426 0.89 +0.3 513,832 - -1.3 513,836 + -0.7 513,837 - -1.7 513,901 + -0.2 514,144 - BT0427 0.46 -1.1 514,245 + BT0427 0.64 -2.6 514,246 - BT0427 0.64 -1.1 514,283 + BT0427 0.70 +0.3 514,284 - BT0427 0.70 +2.3 514,457 - +0.9 514,480 + +0.0 514,481 - -0.9 514,507 - +0.6 514,528 - -2.6 514,672 + +0.3 514,673 - -3.1 514,702 - BT0428 0.12 -0.6 514,775 - BT0428 0.17 +0.7 514,783 - BT0428 0.18 +1.2 514,783 - BT0428 0.18 +0.3 514,859 - BT0428 0.24 +0.3 514,935 - BT0428 0.29 -1.6 514,994 - BT0428 0.34 -2.9 515,067 + BT0428 0.40 +0.4 515,153 - BT0428 0.46 -1.1 515,156 - BT0428 0.46 -3.1 515,158 - BT0428 0.46 +0.2 515,158 - BT0428 0.46 -1.6 515,261 - BT0428 0.54 -0.5 515,298 + BT0428 0.57 -0.7 515,315 + BT0428 0.58 +0.3 515,330 - BT0428 0.60 -0.3 515,363 - BT0428 0.62 -0.1 515,558 - BT0428 0.77 +0.8 515,594 + BT0428 0.80 +2.0 515,702 - BT0428 0.88 +0.3 515,702 - BT0428 0.88 -1.1 515,705 - BT0428 0.88 -0.6 515,746 - -0.6 515,866 + +1.3 515,867 - -2.4 515,870 + +0.3 515,917 - +0.3 516,091 - BT0429 0.38 -1.1 516,118 + BT0429 0.43 -0.7 516,207 - BT0429 0.58 -1.2 516,360 - BT0429 0.84 +0.7 516,391 - BT0429 0.89 -0.7 516,437 - +1.3 516,439 - -1.1 516,463 - +0.7
Or see this region's nucleotide sequence