Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4751 and Pf1N1B4_4752 are separated by 222 nucleotides Pf1N1B4_4752 and Pf1N1B4_4753 are separated by 51 nucleotides Pf1N1B4_4753 and Pf1N1B4_4754 are separated by 106 nucleotides
Pf1N1B4_4751 - Ku domain protein, at 5,248,957 to 5,249,817
_4751
Pf1N1B4_4752 - FIG00962948: hypothetical protein, at 5,250,040 to 5,250,384
_4752
Pf1N1B4_4753 - Soluble aldose sugar dehydrogenase, PQQ-dependent (EC 1.1.5.-), at 5,250,436 to 5,251,587
_4753
Pf1N1B4_4754 - GNAT family acetyltransferase VC2332, at 5,251,694 to 5,252,791
_4754
Position (kb) 5250
5251
5252 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5249.450 kb on + strand at 5249.451 kb on - strand, within Pf1N1B4_4751 at 5249.451 kb on - strand at 5249.483 kb on + strand, within Pf1N1B4_4751 at 5249.484 kb on - strand, within Pf1N1B4_4751 at 5249.484 kb on - strand, within Pf1N1B4_4751 at 5249.487 kb on - strand, within Pf1N1B4_4751 at 5249.487 kb on - strand, within Pf1N1B4_4751 at 5249.603 kb on + strand, within Pf1N1B4_4751 at 5249.604 kb on - strand at 5249.619 kb on - strand, within Pf1N1B4_4751 at 5249.841 kb on - strand at 5249.841 kb on - strand at 5249.841 kb on - strand at 5249.927 kb on - strand at 5249.948 kb on - strand at 5249.951 kb on + strand at 5250.015 kb on - strand at 5250.376 kb on + strand at 5250.377 kb on - strand at 5250.413 kb on - strand at 5250.444 kb on + strand at 5250.526 kb on - strand at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.773 kb on - strand, within Pf1N1B4_4753 at 5250.773 kb on - strand, within Pf1N1B4_4753 at 5250.773 kb on - strand at 5250.773 kb on - strand, within Pf1N1B4_4753 at 5250.949 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.508 kb on + strand at 5251.508 kb on + strand at 5251.534 kb on + strand at 5251.534 kb on + strand at 5251.534 kb on + strand at 5251.535 kb on - strand at 5251.535 kb on - strand at 5251.567 kb on + strand at 5251.567 kb on + strand at 5251.842 kb on + strand, within Pf1N1B4_4754 at 5251.950 kb on - strand, within Pf1N1B4_4754 at 5252.028 kb on - strand, within Pf1N1B4_4754 at 5252.028 kb on - strand, within Pf1N1B4_4754 at 5252.040 kb on - strand, within Pf1N1B4_4754 at 5252.102 kb on + strand at 5252.103 kb on - strand, within Pf1N1B4_4754 at 5252.311 kb on - strand, within Pf1N1B4_4754 at 5252.392 kb on - strand, within Pf1N1B4_4754 at 5252.434 kb on - strand at 5252.578 kb on - strand, within Pf1N1B4_4754
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 5,249,450 + -2.2 5,249,451 - Pf1N1B4_4751 0.57 -1.4 5,249,451 - -0.4 5,249,483 + Pf1N1B4_4751 0.61 +0.4 5,249,484 - Pf1N1B4_4751 0.61 +1.1 5,249,484 - Pf1N1B4_4751 0.61 -2.1 5,249,487 - Pf1N1B4_4751 0.62 +0.4 5,249,487 - Pf1N1B4_4751 0.62 +0.3 5,249,603 + Pf1N1B4_4751 0.75 +0.0 5,249,604 - +2.1 5,249,619 - Pf1N1B4_4751 0.77 +0.6 5,249,841 - +0.4 5,249,841 - -1.6 5,249,841 - -0.7 5,249,927 - -0.3 5,249,948 - +0.9 5,249,951 + -1.7 5,250,015 - -1.1 5,250,376 + +1.2 5,250,377 - -2.1 5,250,413 - -0.1 5,250,444 + -0.3 5,250,526 - -2.1 5,250,772 + Pf1N1B4_4753 0.29 -0.8 5,250,772 + Pf1N1B4_4753 0.29 -0.7 5,250,772 + Pf1N1B4_4753 0.29 -0.4 5,250,772 + Pf1N1B4_4753 0.29 -1.0 5,250,772 + Pf1N1B4_4753 0.29 -0.1 5,250,772 + Pf1N1B4_4753 0.29 -0.3 5,250,773 - Pf1N1B4_4753 0.29 -0.8 5,250,773 - Pf1N1B4_4753 0.29 -3.0 5,250,773 - -1.0 5,250,773 - Pf1N1B4_4753 0.29 -1.3 5,250,949 + Pf1N1B4_4753 0.45 -0.4 5,251,255 + Pf1N1B4_4753 0.71 -1.2 5,251,255 + Pf1N1B4_4753 0.71 +1.0 5,251,255 + Pf1N1B4_4753 0.71 -1.5 5,251,255 + Pf1N1B4_4753 0.71 +0.7 5,251,255 + Pf1N1B4_4753 0.71 -0.5 5,251,255 + Pf1N1B4_4753 0.71 +0.1 5,251,508 + -0.9 5,251,508 + +0.6 5,251,534 + -0.1 5,251,534 + +0.5 5,251,534 + -1.1 5,251,535 - -0.1 5,251,535 - -0.2 5,251,567 + -1.0 5,251,567 + +0.0 5,251,842 + Pf1N1B4_4754 0.13 -1.2 5,251,950 - Pf1N1B4_4754 0.23 -0.8 5,252,028 - Pf1N1B4_4754 0.30 -1.8 5,252,028 - Pf1N1B4_4754 0.30 -0.1 5,252,040 - Pf1N1B4_4754 0.32 -1.3 5,252,102 + -1.3 5,252,103 - Pf1N1B4_4754 0.37 -0.5 5,252,311 - Pf1N1B4_4754 0.56 -1.1 5,252,392 - Pf1N1B4_4754 0.64 -0.6 5,252,434 - +1.5 5,252,578 - Pf1N1B4_4754 0.81 -0.2
Or see this region's nucleotide sequence