Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4163 and Pf1N1B4_4164 are separated by 741 nucleotides Pf1N1B4_4164 and Pf1N1B4_4165 are separated by 234 nucleotides
Pf1N1B4_4163 - FIG00638718: hypothetical protein, at 4,611,436 to 4,612,251
_4163
Pf1N1B4_4164 - hypothetical protein, at 4,612,993 to 4,615,116
_4164
Pf1N1B4_4165 - Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7), at 4,615,351 to 4,616,787
_4165
Position (kb)
4612
4613
4614
4615
4616 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4612.051 kb on + strand, within Pf1N1B4_4163 at 4612.052 kb on - strand, within Pf1N1B4_4163 at 4612.052 kb on - strand, within Pf1N1B4_4163 at 4612.060 kb on + strand, within Pf1N1B4_4163 at 4612.061 kb on - strand, within Pf1N1B4_4163 at 4612.061 kb on - strand, within Pf1N1B4_4163 at 4612.069 kb on + strand, within Pf1N1B4_4163 at 4612.069 kb on + strand, within Pf1N1B4_4163 at 4612.069 kb on + strand, within Pf1N1B4_4163 at 4612.070 kb on - strand, within Pf1N1B4_4163 at 4612.070 kb on - strand, within Pf1N1B4_4163 at 4612.070 kb on - strand, within Pf1N1B4_4163 at 4612.070 kb on - strand, within Pf1N1B4_4163 at 4612.072 kb on + strand, within Pf1N1B4_4163 at 4612.240 kb on - strand at 4612.378 kb on + strand at 4612.412 kb on - strand at 4612.686 kb on + strand at 4612.686 kb on + strand at 4612.770 kb on - strand at 4612.853 kb on - strand at 4612.856 kb on + strand at 4612.857 kb on - strand at 4612.918 kb on - strand at 4612.946 kb on + strand at 4613.038 kb on + strand at 4613.042 kb on - strand at 4613.042 kb on - strand at 4613.042 kb on - strand at 4613.161 kb on + strand at 4613.507 kb on + strand, within Pf1N1B4_4164 at 4613.507 kb on + strand, within Pf1N1B4_4164 at 4613.507 kb on + strand at 4613.507 kb on + strand, within Pf1N1B4_4164 at 4613.508 kb on - strand, within Pf1N1B4_4164 at 4613.508 kb on - strand, within Pf1N1B4_4164 at 4613.687 kb on - strand, within Pf1N1B4_4164 at 4613.687 kb on - strand, within Pf1N1B4_4164 at 4614.018 kb on + strand, within Pf1N1B4_4164 at 4614.018 kb on + strand, within Pf1N1B4_4164 at 4614.104 kb on - strand, within Pf1N1B4_4164 at 4614.214 kb on + strand, within Pf1N1B4_4164 at 4614.214 kb on + strand, within Pf1N1B4_4164 at 4614.214 kb on + strand, within Pf1N1B4_4164 at 4614.214 kb on + strand, within Pf1N1B4_4164 at 4614.214 kb on + strand, within Pf1N1B4_4164 at 4614.214 kb on + strand, within Pf1N1B4_4164 at 4614.215 kb on - strand, within Pf1N1B4_4164 at 4614.230 kb on + strand, within Pf1N1B4_4164 at 4614.286 kb on + strand, within Pf1N1B4_4164 at 4614.306 kb on + strand, within Pf1N1B4_4164 at 4614.400 kb on + strand, within Pf1N1B4_4164 at 4614.496 kb on + strand, within Pf1N1B4_4164 at 4614.497 kb on - strand, within Pf1N1B4_4164 at 4614.497 kb on - strand, within Pf1N1B4_4164 at 4614.497 kb on - strand at 4614.525 kb on + strand, within Pf1N1B4_4164 at 4614.525 kb on + strand, within Pf1N1B4_4164 at 4614.525 kb on + strand, within Pf1N1B4_4164 at 4614.526 kb on - strand, within Pf1N1B4_4164 at 4614.526 kb on - strand, within Pf1N1B4_4164 at 4614.526 kb on - strand at 4614.526 kb on - strand, within Pf1N1B4_4164 at 4614.526 kb on - strand, within Pf1N1B4_4164 at 4614.592 kb on + strand, within Pf1N1B4_4164 at 4614.592 kb on + strand, within Pf1N1B4_4164 at 4614.593 kb on - strand, within Pf1N1B4_4164 at 4614.674 kb on + strand, within Pf1N1B4_4164 at 4614.675 kb on - strand, within Pf1N1B4_4164 at 4614.718 kb on - strand, within Pf1N1B4_4164 at 4614.738 kb on + strand, within Pf1N1B4_4164 at 4614.779 kb on - strand, within Pf1N1B4_4164 at 4614.835 kb on + strand, within Pf1N1B4_4164 at 4614.881 kb on - strand at 4614.994 kb on + strand at 4615.039 kb on + strand at 4615.039 kb on + strand at 4615.039 kb on + strand at 4615.040 kb on - strand at 4615.069 kb on + strand at 4615.070 kb on - strand at 4615.070 kb on - strand at 4615.135 kb on - strand at 4615.337 kb on - strand at 4615.616 kb on + strand at 4615.616 kb on + strand at 4615.617 kb on - strand, within Pf1N1B4_4165 at 4615.664 kb on - strand, within Pf1N1B4_4165 at 4615.664 kb on - strand, within Pf1N1B4_4165 at 4615.710 kb on + strand, within Pf1N1B4_4165 at 4615.754 kb on - strand, within Pf1N1B4_4165 at 4615.766 kb on + strand, within Pf1N1B4_4165 at 4615.797 kb on + strand, within Pf1N1B4_4165 at 4615.798 kb on - strand at 4615.928 kb on - strand at 4616.093 kb on + strand, within Pf1N1B4_4165 at 4616.094 kb on - strand, within Pf1N1B4_4165 at 4616.097 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 4,612,051 + Pf1N1B4_4163 0.75 +0.3 4,612,052 - Pf1N1B4_4163 0.75 +0.5 4,612,052 - Pf1N1B4_4163 0.75 +1.0 4,612,060 + Pf1N1B4_4163 0.76 -0.1 4,612,061 - Pf1N1B4_4163 0.77 -0.4 4,612,061 - Pf1N1B4_4163 0.77 -0.6 4,612,069 + Pf1N1B4_4163 0.78 +0.4 4,612,069 + Pf1N1B4_4163 0.78 -0.2 4,612,069 + Pf1N1B4_4163 0.78 +1.0 4,612,070 - Pf1N1B4_4163 0.78 -0.3 4,612,070 - Pf1N1B4_4163 0.78 +0.0 4,612,070 - Pf1N1B4_4163 0.78 +0.3 4,612,070 - Pf1N1B4_4163 0.78 -0.5 4,612,072 + Pf1N1B4_4163 0.78 +0.5 4,612,240 - -0.0 4,612,378 + +0.3 4,612,412 - -0.2 4,612,686 + -1.7 4,612,686 + -0.1 4,612,770 - +0.6 4,612,853 - -0.1 4,612,856 + -0.6 4,612,857 - +1.1 4,612,918 - -0.0 4,612,946 + +0.4 4,613,038 + -0.2 4,613,042 - -0.3 4,613,042 - -0.9 4,613,042 - -2.8 4,613,161 + +0.7 4,613,507 + Pf1N1B4_4164 0.24 -1.1 4,613,507 + Pf1N1B4_4164 0.24 -0.6 4,613,507 + -0.9 4,613,507 + Pf1N1B4_4164 0.24 -0.6 4,613,508 - Pf1N1B4_4164 0.24 +0.9 4,613,508 - Pf1N1B4_4164 0.24 -0.1 4,613,687 - Pf1N1B4_4164 0.33 -0.9 4,613,687 - Pf1N1B4_4164 0.33 -0.2 4,614,018 + Pf1N1B4_4164 0.48 -0.3 4,614,018 + Pf1N1B4_4164 0.48 -0.4 4,614,104 - Pf1N1B4_4164 0.52 +0.1 4,614,214 + Pf1N1B4_4164 0.57 +0.1 4,614,214 + Pf1N1B4_4164 0.57 -1.5 4,614,214 + Pf1N1B4_4164 0.57 -3.5 4,614,214 + Pf1N1B4_4164 0.57 -1.2 4,614,214 + Pf1N1B4_4164 0.57 -0.7 4,614,214 + Pf1N1B4_4164 0.57 -0.5 4,614,215 - Pf1N1B4_4164 0.58 -0.5 4,614,230 + Pf1N1B4_4164 0.58 -1.6 4,614,286 + Pf1N1B4_4164 0.61 -0.7 4,614,306 + Pf1N1B4_4164 0.62 +0.1 4,614,400 + Pf1N1B4_4164 0.66 -1.5 4,614,496 + Pf1N1B4_4164 0.71 +0.2 4,614,497 - Pf1N1B4_4164 0.71 +0.8 4,614,497 - Pf1N1B4_4164 0.71 +0.2 4,614,497 - -0.2 4,614,525 + Pf1N1B4_4164 0.72 -0.2 4,614,525 + Pf1N1B4_4164 0.72 -0.7 4,614,525 + Pf1N1B4_4164 0.72 +1.2 4,614,526 - Pf1N1B4_4164 0.72 +0.2 4,614,526 - Pf1N1B4_4164 0.72 +0.1 4,614,526 - -2.0 4,614,526 - Pf1N1B4_4164 0.72 +0.1 4,614,526 - Pf1N1B4_4164 0.72 +0.2 4,614,592 + Pf1N1B4_4164 0.75 +0.5 4,614,592 + Pf1N1B4_4164 0.75 +1.1 4,614,593 - Pf1N1B4_4164 0.75 -1.0 4,614,674 + Pf1N1B4_4164 0.79 -0.3 4,614,675 - Pf1N1B4_4164 0.79 +1.6 4,614,718 - Pf1N1B4_4164 0.81 -0.0 4,614,738 + Pf1N1B4_4164 0.82 -0.6 4,614,779 - Pf1N1B4_4164 0.84 -1.0 4,614,835 + Pf1N1B4_4164 0.87 -0.6 4,614,881 - +0.1 4,614,994 + +0.5 4,615,039 + -0.7 4,615,039 + +0.4 4,615,039 + -0.2 4,615,040 - -0.9 4,615,069 + -0.3 4,615,070 - -2.5 4,615,070 - +0.0 4,615,135 - +0.6 4,615,337 - -1.6 4,615,616 + -1.3 4,615,616 + -0.3 4,615,617 - Pf1N1B4_4165 0.19 -0.1 4,615,664 - Pf1N1B4_4165 0.22 +0.4 4,615,664 - Pf1N1B4_4165 0.22 -1.0 4,615,710 + Pf1N1B4_4165 0.25 -0.1 4,615,754 - Pf1N1B4_4165 0.28 -0.2 4,615,766 + Pf1N1B4_4165 0.29 +0.6 4,615,797 + Pf1N1B4_4165 0.31 +1.5 4,615,798 - -1.9 4,615,928 - -2.0 4,616,093 + Pf1N1B4_4165 0.52 -0.9 4,616,094 - Pf1N1B4_4165 0.52 +0.4 4,616,097 - +0.8
Or see this region's nucleotide sequence