Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4047

Experiment: nitrogen source nitrate 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4046 and Pf1N1B4_4047 are separated by 127 nucleotidesPf1N1B4_4047 and Pf1N1B4_4048 overlap by 4 nucleotides Pf1N1B4_4046 - Adenylosuccinate lyase (EC 4.3.2.2), at 4,498,338 to 4,499,708 _4046 Pf1N1B4_4047 - FIG002903: a protein of unknown function perhaps involved in purine metabolism, at 4,499,836 to 4,500,459 _4047 Pf1N1B4_4048 - tRNA-specific 2-thiouridylase MnmA, at 4,500,456 to 4,501,589 _4048 Position (kb) 4499 4500 4501Strain fitness (log2 ratio) -1 0 1at 4499.795 kb on - strandat 4499.795 kb on - strandat 4499.795 kb on - strandat 4499.811 kb on + strandat 4499.821 kb on + strandat 4499.868 kb on + strandat 4499.950 kb on + strandat 4499.954 kb on - strand, within Pf1N1B4_4047at 4499.954 kb on - strand, within Pf1N1B4_4047at 4499.954 kb on - strand, within Pf1N1B4_4047at 4500.053 kb on + strand, within Pf1N1B4_4047at 4500.053 kb on + strand, within Pf1N1B4_4047at 4500.054 kb on - strand, within Pf1N1B4_4047at 4500.054 kb on - strand, within Pf1N1B4_4047at 4500.054 kb on - strand, within Pf1N1B4_4047at 4500.299 kb on + strand, within Pf1N1B4_4047at 4500.299 kb on + strand, within Pf1N1B4_4047at 4500.299 kb on + strand, within Pf1N1B4_4047at 4500.300 kb on - strand, within Pf1N1B4_4047at 4500.300 kb on - strand, within Pf1N1B4_4047

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM
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4,499,795 - -0.8
4,499,795 - +1.5
4,499,795 - -0.8
4,499,811 + -1.6
4,499,821 + -1.6
4,499,868 + -0.4
4,499,950 + +0.7
4,499,954 - Pf1N1B4_4047 0.19 +0.3
4,499,954 - Pf1N1B4_4047 0.19 +0.8
4,499,954 - Pf1N1B4_4047 0.19 -1.3
4,500,053 + Pf1N1B4_4047 0.35 -0.9
4,500,053 + Pf1N1B4_4047 0.35 -1.6
4,500,054 - Pf1N1B4_4047 0.35 +1.1
4,500,054 - Pf1N1B4_4047 0.35 +0.3
4,500,054 - Pf1N1B4_4047 0.35 +0.2
4,500,299 + Pf1N1B4_4047 0.74 +1.2
4,500,299 + Pf1N1B4_4047 0.74 +0.5
4,500,299 + Pf1N1B4_4047 0.74 +0.5
4,500,300 - Pf1N1B4_4047 0.74 -0.1
4,500,300 - Pf1N1B4_4047 0.74 +0.7

Or see this region's nucleotide sequence