Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3184 and Pf1N1B4_3185 are separated by 199 nucleotides Pf1N1B4_3185 and Pf1N1B4_3186 are separated by 5 nucleotides Pf1N1B4_3186 and Pf1N1B4_3187 are separated by 114 nucleotides Pf1N1B4_3187 and Pf1N1B4_3188 are separated by 4 nucleotides
Pf1N1B4_3184 - Isochorismatase (EC 3.3.2.1), at 3,568,359 to 3,568,916
_3184
Pf1N1B4_3185 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 3,569,116 to 3,569,325
_3185
Pf1N1B4_3186 - Conserved secreted protein, at 3,569,331 to 3,569,816
_3186
Pf1N1B4_3187 - UPF0225 protein YchJ, at 3,569,931 to 3,570,407
_3187
Pf1N1B4_3188 - FIG00953202: hypothetical protein, at 3,570,412 to 3,570,909
_3188
Position (kb)
3569
3570 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3568.787 kb on + strand, within Pf1N1B4_3184 at 3568.787 kb on + strand, within Pf1N1B4_3184 at 3568.788 kb on - strand, within Pf1N1B4_3184 at 3568.806 kb on + strand, within Pf1N1B4_3184 at 3568.807 kb on - strand, within Pf1N1B4_3184 at 3568.872 kb on + strand at 3568.872 kb on + strand at 3568.914 kb on + strand at 3568.954 kb on + strand at 3568.954 kb on + strand at 3569.013 kb on + strand at 3569.013 kb on + strand at 3569.025 kb on + strand at 3569.344 kb on - strand at 3569.358 kb on + strand at 3569.386 kb on + strand, within Pf1N1B4_3186 at 3569.386 kb on + strand, within Pf1N1B4_3186 at 3569.386 kb on + strand, within Pf1N1B4_3186 at 3569.387 kb on - strand, within Pf1N1B4_3186 at 3569.387 kb on - strand, within Pf1N1B4_3186 at 3569.387 kb on - strand at 3569.387 kb on - strand, within Pf1N1B4_3186 at 3569.387 kb on - strand, within Pf1N1B4_3186 at 3569.401 kb on - strand, within Pf1N1B4_3186 at 3569.401 kb on - strand, within Pf1N1B4_3186 at 3569.513 kb on + strand, within Pf1N1B4_3186 at 3569.513 kb on + strand, within Pf1N1B4_3186 at 3569.514 kb on - strand, within Pf1N1B4_3186 at 3569.519 kb on - strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.676 kb on + strand, within Pf1N1B4_3186 at 3569.676 kb on + strand, within Pf1N1B4_3186 at 3569.793 kb on + strand at 3569.793 kb on + strand at 3569.793 kb on + strand at 3569.793 kb on + strand at 3569.794 kb on - strand at 3569.824 kb on + strand at 3569.825 kb on - strand at 3569.845 kb on + strand at 3569.845 kb on + strand at 3569.845 kb on + strand at 3569.879 kb on + strand at 3569.879 kb on + strand at 3569.880 kb on - strand at 3569.944 kb on + strand at 3570.000 kb on - strand, within Pf1N1B4_3187 at 3570.000 kb on - strand, within Pf1N1B4_3187 at 3570.000 kb on - strand, within Pf1N1B4_3187 at 3570.037 kb on + strand, within Pf1N1B4_3187 at 3570.037 kb on + strand, within Pf1N1B4_3187 at 3570.038 kb on - strand, within Pf1N1B4_3187 at 3570.038 kb on - strand, within Pf1N1B4_3187 at 3570.058 kb on - strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.265 kb on + strand, within Pf1N1B4_3187 at 3570.266 kb on - strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.286 kb on + strand, within Pf1N1B4_3187 at 3570.286 kb on + strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187 at 3570.346 kb on + strand, within Pf1N1B4_3187 at 3570.401 kb on + strand at 3570.401 kb on + strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.422 kb on + strand at 3570.422 kb on + strand at 3570.423 kb on - strand at 3570.423 kb on - strand at 3570.483 kb on + strand, within Pf1N1B4_3188 at 3570.483 kb on + strand, within Pf1N1B4_3188 at 3570.484 kb on - strand, within Pf1N1B4_3188 at 3570.484 kb on - strand, within Pf1N1B4_3188 at 3570.484 kb on - strand, within Pf1N1B4_3188 at 3570.485 kb on + strand at 3570.485 kb on + strand, within Pf1N1B4_3188 at 3570.485 kb on + strand, within Pf1N1B4_3188 at 3570.485 kb on + strand, within Pf1N1B4_3188 at 3570.486 kb on - strand, within Pf1N1B4_3188 at 3570.486 kb on - strand, within Pf1N1B4_3188 at 3570.500 kb on + strand, within Pf1N1B4_3188 at 3570.500 kb on + strand, within Pf1N1B4_3188 at 3570.500 kb on + strand, within Pf1N1B4_3188 at 3570.500 kb on + strand, within Pf1N1B4_3188 at 3570.500 kb on + strand, within Pf1N1B4_3188 at 3570.501 kb on - strand, within Pf1N1B4_3188 at 3570.504 kb on + strand, within Pf1N1B4_3188 at 3570.504 kb on + strand, within Pf1N1B4_3188 at 3570.505 kb on - strand, within Pf1N1B4_3188 at 3570.517 kb on + strand, within Pf1N1B4_3188 at 3570.517 kb on + strand, within Pf1N1B4_3188 at 3570.517 kb on + strand, within Pf1N1B4_3188 at 3570.518 kb on - strand, within Pf1N1B4_3188 at 3570.518 kb on - strand, within Pf1N1B4_3188 at 3570.518 kb on - strand, within Pf1N1B4_3188 at 3570.518 kb on - strand, within Pf1N1B4_3188
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 3,568,787 + Pf1N1B4_3184 0.77 -1.8 3,568,787 + Pf1N1B4_3184 0.77 +1.1 3,568,788 - Pf1N1B4_3184 0.77 -0.3 3,568,806 + Pf1N1B4_3184 0.80 -2.9 3,568,807 - Pf1N1B4_3184 0.80 -0.2 3,568,872 + -0.5 3,568,872 + -1.2 3,568,914 + -1.2 3,568,954 + +0.2 3,568,954 + +0.4 3,569,013 + +0.6 3,569,013 + -0.7 3,569,025 + -0.5 3,569,344 - -2.7 3,569,358 + +0.3 3,569,386 + Pf1N1B4_3186 0.11 +0.1 3,569,386 + Pf1N1B4_3186 0.11 -1.9 3,569,386 + Pf1N1B4_3186 0.11 +0.3 3,569,387 - Pf1N1B4_3186 0.12 -2.7 3,569,387 - Pf1N1B4_3186 0.12 -0.4 3,569,387 - +1.5 3,569,387 - Pf1N1B4_3186 0.12 -0.2 3,569,387 - Pf1N1B4_3186 0.12 +0.7 3,569,401 - Pf1N1B4_3186 0.14 +0.0 3,569,401 - Pf1N1B4_3186 0.14 +1.8 3,569,513 + Pf1N1B4_3186 0.37 +0.1 3,569,513 + Pf1N1B4_3186 0.37 +0.9 3,569,514 - Pf1N1B4_3186 0.38 -0.4 3,569,519 - Pf1N1B4_3186 0.39 +1.5 3,569,577 + Pf1N1B4_3186 0.51 +0.3 3,569,577 + Pf1N1B4_3186 0.51 +1.9 3,569,577 + Pf1N1B4_3186 0.51 +0.1 3,569,577 + Pf1N1B4_3186 0.51 -0.1 3,569,577 + Pf1N1B4_3186 0.51 -0.3 3,569,577 + Pf1N1B4_3186 0.51 +0.7 3,569,577 + Pf1N1B4_3186 0.51 +0.1 3,569,577 + Pf1N1B4_3186 0.51 -0.1 3,569,577 + Pf1N1B4_3186 0.51 -0.1 3,569,577 + Pf1N1B4_3186 0.51 -0.6 3,569,577 + Pf1N1B4_3186 0.51 -0.4 3,569,577 + Pf1N1B4_3186 0.51 -1.0 3,569,577 + Pf1N1B4_3186 0.51 +0.7 3,569,577 + Pf1N1B4_3186 0.51 -1.0 3,569,577 + Pf1N1B4_3186 0.51 +0.1 3,569,578 - Pf1N1B4_3186 0.51 +0.0 3,569,578 - Pf1N1B4_3186 0.51 -1.7 3,569,578 - Pf1N1B4_3186 0.51 +0.3 3,569,578 - Pf1N1B4_3186 0.51 -0.0 3,569,578 - Pf1N1B4_3186 0.51 +0.4 3,569,578 - Pf1N1B4_3186 0.51 -0.3 3,569,578 - Pf1N1B4_3186 0.51 -0.5 3,569,578 - Pf1N1B4_3186 0.51 -1.1 3,569,676 + Pf1N1B4_3186 0.71 +0.0 3,569,676 + Pf1N1B4_3186 0.71 -0.4 3,569,793 + -1.3 3,569,793 + -0.9 3,569,793 + +0.2 3,569,793 + -0.0 3,569,794 - -1.3 3,569,824 + -2.5 3,569,825 - +1.0 3,569,845 + +0.2 3,569,845 + -0.2 3,569,845 + -0.8 3,569,879 + -0.1 3,569,879 + -0.1 3,569,880 - +0.0 3,569,944 + -0.3 3,570,000 - Pf1N1B4_3187 0.14 -1.7 3,570,000 - Pf1N1B4_3187 0.14 +0.1 3,570,000 - Pf1N1B4_3187 0.14 +0.3 3,570,037 + Pf1N1B4_3187 0.22 +1.3 3,570,037 + Pf1N1B4_3187 0.22 +0.0 3,570,038 - Pf1N1B4_3187 0.22 +0.1 3,570,038 - Pf1N1B4_3187 0.22 +0.5 3,570,058 - Pf1N1B4_3187 0.27 -1.3 3,570,261 + Pf1N1B4_3187 0.69 -1.1 3,570,261 + Pf1N1B4_3187 0.69 -1.1 3,570,261 + Pf1N1B4_3187 0.69 +0.6 3,570,261 + Pf1N1B4_3187 0.69 +0.2 3,570,262 - Pf1N1B4_3187 0.69 +0.2 3,570,262 - Pf1N1B4_3187 0.69 -0.7 3,570,262 - Pf1N1B4_3187 0.69 -1.7 3,570,262 - Pf1N1B4_3187 0.69 -0.5 3,570,265 + Pf1N1B4_3187 0.70 +0.2 3,570,266 - Pf1N1B4_3187 0.70 -0.4 3,570,282 + Pf1N1B4_3187 0.74 +0.2 3,570,282 + Pf1N1B4_3187 0.74 +1.6 3,570,282 + Pf1N1B4_3187 0.74 +1.0 3,570,282 + Pf1N1B4_3187 0.74 -0.8 3,570,282 + Pf1N1B4_3187 0.74 +0.8 3,570,282 + Pf1N1B4_3187 0.74 +0.5 3,570,282 + Pf1N1B4_3187 0.74 +1.4 3,570,283 - Pf1N1B4_3187 0.74 +0.7 3,570,283 - -2.0 3,570,283 - Pf1N1B4_3187 0.74 -0.3 3,570,283 - Pf1N1B4_3187 0.74 +0.7 3,570,283 - Pf1N1B4_3187 0.74 +0.6 3,570,283 - Pf1N1B4_3187 0.74 -1.1 3,570,283 - Pf1N1B4_3187 0.74 -0.5 3,570,283 - Pf1N1B4_3187 0.74 +2.4 3,570,283 - Pf1N1B4_3187 0.74 -0.6 3,570,286 + Pf1N1B4_3187 0.74 +0.5 3,570,286 + Pf1N1B4_3187 0.74 +0.0 3,570,287 - Pf1N1B4_3187 0.75 +0.8 3,570,287 - Pf1N1B4_3187 0.75 +0.6 3,570,287 - Pf1N1B4_3187 0.75 -0.9 3,570,287 - Pf1N1B4_3187 0.75 +0.1 3,570,346 + Pf1N1B4_3187 0.87 -2.5 3,570,401 + -0.4 3,570,401 + +1.0 3,570,402 - -0.0 3,570,402 - -1.2 3,570,402 - -1.5 3,570,402 - -0.0 3,570,402 - +0.2 3,570,402 - +1.1 3,570,402 - -2.0 3,570,402 - +0.3 3,570,402 - -0.7 3,570,422 + +0.3 3,570,422 + -0.5 3,570,423 - -1.3 3,570,423 - -1.1 3,570,483 + Pf1N1B4_3188 0.14 -0.1 3,570,483 + Pf1N1B4_3188 0.14 +0.7 3,570,484 - Pf1N1B4_3188 0.14 +1.7 3,570,484 - Pf1N1B4_3188 0.14 -0.4 3,570,484 - Pf1N1B4_3188 0.14 +0.5 3,570,485 + -0.8 3,570,485 + Pf1N1B4_3188 0.15 +1.5 3,570,485 + Pf1N1B4_3188 0.15 +0.4 3,570,485 + Pf1N1B4_3188 0.15 +1.0 3,570,486 - Pf1N1B4_3188 0.15 -0.5 3,570,486 - Pf1N1B4_3188 0.15 +1.2 3,570,500 + Pf1N1B4_3188 0.18 -2.0 3,570,500 + Pf1N1B4_3188 0.18 +1.0 3,570,500 + Pf1N1B4_3188 0.18 -1.0 3,570,500 + Pf1N1B4_3188 0.18 +0.6 3,570,500 + Pf1N1B4_3188 0.18 +0.3 3,570,501 - Pf1N1B4_3188 0.18 -0.3 3,570,504 + Pf1N1B4_3188 0.18 +0.2 3,570,504 + Pf1N1B4_3188 0.18 +0.7 3,570,505 - Pf1N1B4_3188 0.19 -0.8 3,570,517 + Pf1N1B4_3188 0.21 +0.3 3,570,517 + Pf1N1B4_3188 0.21 -1.7 3,570,517 + Pf1N1B4_3188 0.21 +1.2 3,570,518 - Pf1N1B4_3188 0.21 -0.3 3,570,518 - Pf1N1B4_3188 0.21 -2.3 3,570,518 - Pf1N1B4_3188 0.21 +0.6 3,570,518 - Pf1N1B4_3188 0.21 -0.2
Or see this region's nucleotide sequence