Experiment: BHIS with Deoxycholic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3922 and BT3923 are separated by 7 nucleotides BT3923 and BT3924 are separated by 0 nucleotides BT3924 and BT3925 are separated by 223 nucleotides BT3925 and BT3926 are separated by 18 nucleotides
BT3922: BT3922 - putative ThiJ family intracellular protease/amidase (NCBI ptt file), at 5,095,823 to 5,096,374
BT3922
BT3923: BT3923 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (NCBI ptt file), at 5,096,382 to 5,097,041
BT3923
BT3924: BT3924 - ATP-dependent DNA helicase recG (NCBI ptt file), at 5,097,042 to 5,099,138
BT3924
BT3925: BT3925 - nucleoside diphosphate kinase (NCBI ptt file), at 5,099,362 to 5,099,826
BT3925
BT3926: BT3926 - putative peptidase (NCBI ptt file), at 5,099,845 to 5,100,717
BT3926
Position (kb) 5097
5098
5099
5100 Strain fitness (log2 ratio) -2
-1
0
1 at 5096.053 kb on + strand, within BT3922 at 5096.257 kb on + strand, within BT3922 at 5096.257 kb on + strand, within BT3922 at 5096.257 kb on + strand, within BT3922 at 5096.287 kb on + strand, within BT3922 at 5096.349 kb on + strand at 5096.843 kb on + strand, within BT3923 at 5098.443 kb on - strand, within BT3924 at 5099.339 kb on + strand at 5099.445 kb on + strand, within BT3925 at 5099.495 kb on + strand, within BT3925 at 5099.495 kb on + strand, within BT3925 at 5099.587 kb on + strand, within BT3925 at 5099.619 kb on + strand, within BT3925 at 5099.637 kb on + strand, within BT3925 at 5099.709 kb on + strand, within BT3925 at 5099.771 kb on + strand, within BT3925 at 5099.806 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Deoxycholic 0.2 mg/ml remove 5,096,053 + BT3922 0.42 +0.5 5,096,257 + BT3922 0.79 -0.1 5,096,257 + BT3922 0.79 -0.5 5,096,257 + BT3922 0.79 -1.1 5,096,287 + BT3922 0.84 -0.5 5,096,349 + +0.3 5,096,843 + BT3923 0.70 +0.5 5,098,443 - BT3924 0.67 -1.9 5,099,339 + -1.5 5,099,445 + BT3925 0.18 +0.7 5,099,495 + BT3925 0.29 -2.3 5,099,495 + BT3925 0.29 -1.5 5,099,587 + BT3925 0.48 -1.9 5,099,619 + BT3925 0.55 -0.5 5,099,637 + BT3925 0.59 +0.5 5,099,709 + BT3925 0.75 +0.3 5,099,771 + BT3925 0.88 -2.2 5,099,806 + -1.9
Or see this region's nucleotide sequence