Experiment: BHIS with Deoxycholic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3868 and BT3869 are separated by 146 nucleotides BT3869 and BT3870 are separated by 5 nucleotides BT3870 and tRNA-Val are separated by 235 nucleotides tRNA-Val and tRNA-Val are separated by 29 nucleotides
BT3868: BT3868 - beta-N-hexosaminidase, glycosyl hyrolase family 20 (NCBI ptt file), at 5,039,698 to 5,041,683
BT3868
BT3869: BT3869 - conserved hypothetical protein (NCBI ptt file), at 5,041,830 to 5,042,603
BT3869
BT3870: BT3870 - putative maltose O-acetyltransferase (NCBI ptt file), at 5,042,609 to 5,043,160
BT3870
BT_t62: tRNA-Val - tRNA-Val (RefSeq), at 5,043,396 to 5,043,473
tRNA-Val
BT_t63: tRNA-Val - tRNA-Val (RefSeq), at 5,043,503 to 5,043,580
tRNA-Val
Position (kb)
5041
5042
5043 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5041.013 kb on + strand, within BT3868 at 5041.043 kb on + strand, within BT3868 at 5041.043 kb on + strand, within BT3868 at 5041.070 kb on + strand, within BT3868 at 5041.070 kb on + strand, within BT3868 at 5041.125 kb on - strand, within BT3868 at 5041.125 kb on - strand, within BT3868 at 5041.154 kb on + strand, within BT3868 at 5041.187 kb on - strand, within BT3868 at 5041.187 kb on - strand, within BT3868 at 5041.187 kb on - strand, within BT3868 at 5041.327 kb on + strand, within BT3868 at 5041.457 kb on + strand, within BT3868 at 5041.490 kb on - strand at 5041.546 kb on - strand at 5041.546 kb on - strand at 5041.620 kb on + strand at 5041.667 kb on - strand at 5041.733 kb on - strand at 5041.838 kb on - strand at 5042.070 kb on + strand, within BT3869 at 5042.083 kb on + strand, within BT3869 at 5042.291 kb on - strand, within BT3869 at 5042.438 kb on - strand, within BT3869 at 5042.445 kb on + strand, within BT3869 at 5042.445 kb on + strand, within BT3869 at 5042.582 kb on + strand at 5042.582 kb on + strand at 5042.583 kb on - strand at 5042.602 kb on - strand at 5042.620 kb on - strand at 5042.651 kb on - strand at 5042.651 kb on - strand at 5042.726 kb on + strand, within BT3870 at 5042.726 kb on + strand, within BT3870 at 5042.726 kb on + strand, within BT3870 at 5042.863 kb on + strand, within BT3870 at 5042.863 kb on + strand, within BT3870 at 5042.882 kb on + strand, within BT3870 at 5042.882 kb on + strand, within BT3870 at 5042.882 kb on + strand, within BT3870 at 5042.883 kb on - strand, within BT3870 at 5042.883 kb on - strand, within BT3870 at 5042.884 kb on + strand, within BT3870 at 5042.903 kb on - strand, within BT3870 at 5042.930 kb on + strand, within BT3870 at 5042.930 kb on + strand, within BT3870 at 5042.930 kb on + strand, within BT3870 at 5042.931 kb on - strand, within BT3870 at 5042.981 kb on + strand, within BT3870 at 5043.583 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Deoxycholic 0.2 mg/ml remove 5,041,013 + BT3868 0.66 -0.0 5,041,043 + BT3868 0.68 +2.3 5,041,043 + BT3868 0.68 +1.1 5,041,070 + BT3868 0.69 -0.0 5,041,070 + BT3868 0.69 -0.3 5,041,125 - BT3868 0.72 +0.4 5,041,125 - BT3868 0.72 -0.4 5,041,154 + BT3868 0.73 -1.0 5,041,187 - BT3868 0.75 -2.7 5,041,187 - BT3868 0.75 -0.4 5,041,187 - BT3868 0.75 -0.8 5,041,327 + BT3868 0.82 -0.0 5,041,457 + BT3868 0.89 -1.6 5,041,490 - +0.1 5,041,546 - -0.5 5,041,546 - -0.7 5,041,620 + -0.6 5,041,667 - +0.2 5,041,733 - -1.2 5,041,838 - -0.3 5,042,070 + BT3869 0.31 -1.4 5,042,083 + BT3869 0.33 -1.6 5,042,291 - BT3869 0.60 +0.4 5,042,438 - BT3869 0.79 +0.1 5,042,445 + BT3869 0.79 -0.7 5,042,445 + BT3869 0.79 +0.6 5,042,582 + +0.2 5,042,582 + -0.8 5,042,583 - -1.7 5,042,602 - -0.8 5,042,620 - -1.5 5,042,651 - -1.6 5,042,651 - -0.8 5,042,726 + BT3870 0.21 -0.7 5,042,726 + BT3870 0.21 -0.5 5,042,726 + BT3870 0.21 +1.5 5,042,863 + BT3870 0.46 -2.9 5,042,863 + BT3870 0.46 -0.5 5,042,882 + BT3870 0.49 +0.9 5,042,882 + BT3870 0.49 -0.0 5,042,882 + BT3870 0.49 +2.1 5,042,883 - BT3870 0.50 -0.5 5,042,883 - BT3870 0.50 -1.6 5,042,884 + BT3870 0.50 -1.2 5,042,903 - BT3870 0.53 -0.0 5,042,930 + BT3870 0.58 +1.5 5,042,930 + BT3870 0.58 -1.5 5,042,930 + BT3870 0.58 -1.8 5,042,931 - BT3870 0.58 -1.4 5,042,981 + BT3870 0.67 -0.9 5,043,583 + -1.5
Or see this region's nucleotide sequence