Experiment: BHIS with Deoxycholic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3399 and BT3400 are separated by 39 nucleotides BT3400 and BT3401 are separated by 252 nucleotides BT3401 and BT3402 are separated by 154 nucleotides BT3402 and BT3403 are separated by 2 nucleotides
BT3399: BT3399 - putative zinc protease (NCBI ptt file), at 4,378,761 to 4,379,459
BT3399
BT3400: BT3400 - hypothetical protein (NCBI ptt file), at 4,379,499 to 4,379,894
BT3400
BT3401: BT3401 - conserved hypothetical protein (NCBI ptt file), at 4,380,147 to 4,381,292
BT3401
BT3402: BT3402 - conserved hypothetical protein (NCBI ptt file), at 4,381,447 to 4,381,914
BT3402
BT3403: BT3403 - putative nitrogen utilization substance protein (NCBI ptt file), at 4,381,917 to 4,383,236
BT3403
Position (kb)
4380
4381
4382 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4379.212 kb on - strand, within BT3399 at 4379.228 kb on - strand, within BT3399 at 4379.274 kb on - strand, within BT3399 at 4379.287 kb on - strand, within BT3399 at 4379.442 kb on + strand at 4379.455 kb on + strand at 4379.456 kb on - strand at 4379.456 kb on - strand at 4379.456 kb on - strand at 4379.458 kb on - strand at 4379.488 kb on + strand at 4379.500 kb on + strand at 4379.501 kb on - strand at 4379.505 kb on - strand at 4379.595 kb on - strand, within BT3400 at 4379.601 kb on + strand, within BT3400 at 4379.745 kb on - strand, within BT3400 at 4379.745 kb on - strand, within BT3400 at 4379.747 kb on - strand, within BT3400 at 4379.758 kb on + strand, within BT3400 at 4379.794 kb on - strand, within BT3400 at 4379.895 kb on - strand at 4379.904 kb on - strand at 4379.930 kb on - strand at 4380.039 kb on - strand at 4380.039 kb on - strand at 4380.039 kb on - strand at 4380.043 kb on + strand at 4380.043 kb on + strand at 4380.043 kb on + strand at 4380.071 kb on + strand at 4380.072 kb on - strand at 4380.091 kb on + strand at 4380.091 kb on + strand at 4380.092 kb on - strand at 4380.092 kb on - strand at 4380.092 kb on - strand at 4380.092 kb on - strand at 4380.092 kb on - strand at 4380.109 kb on + strand at 4380.110 kb on - strand at 4380.110 kb on - strand at 4380.122 kb on - strand at 4380.163 kb on + strand at 4380.163 kb on + strand at 4380.180 kb on + strand at 4380.188 kb on + strand at 4380.192 kb on - strand at 4380.192 kb on - strand at 4380.203 kb on + strand at 4380.215 kb on + strand at 4380.269 kb on + strand, within BT3401 at 4380.299 kb on + strand, within BT3401 at 4380.305 kb on + strand, within BT3401 at 4380.306 kb on - strand, within BT3401 at 4380.306 kb on - strand, within BT3401 at 4380.306 kb on - strand, within BT3401 at 4380.311 kb on - strand, within BT3401 at 4380.329 kb on + strand, within BT3401 at 4380.387 kb on + strand, within BT3401 at 4380.387 kb on + strand, within BT3401 at 4380.392 kb on + strand, within BT3401 at 4380.431 kb on - strand, within BT3401 at 4380.518 kb on + strand, within BT3401 at 4380.518 kb on + strand, within BT3401 at 4380.611 kb on - strand, within BT3401 at 4380.649 kb on - strand, within BT3401 at 4380.660 kb on - strand, within BT3401 at 4380.664 kb on - strand, within BT3401 at 4380.700 kb on - strand, within BT3401 at 4380.702 kb on - strand, within BT3401 at 4380.771 kb on - strand, within BT3401 at 4380.819 kb on - strand, within BT3401 at 4380.826 kb on + strand, within BT3401 at 4380.858 kb on - strand, within BT3401 at 4380.917 kb on + strand, within BT3401 at 4380.940 kb on - strand, within BT3401 at 4380.958 kb on - strand, within BT3401 at 4380.987 kb on + strand, within BT3401 at 4381.009 kb on - strand, within BT3401 at 4381.066 kb on - strand, within BT3401 at 4381.167 kb on - strand, within BT3401 at 4381.291 kb on - strand at 4381.291 kb on - strand at 4381.294 kb on + strand at 4381.294 kb on + strand at 4381.295 kb on - strand at 4381.313 kb on + strand at 4381.314 kb on - strand at 4381.315 kb on + strand at 4381.316 kb on - strand at 4381.332 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Deoxycholic 0.2 mg/ml remove 4,379,212 - BT3399 0.65 -1.0 4,379,228 - BT3399 0.67 +0.8 4,379,274 - BT3399 0.73 -0.5 4,379,287 - BT3399 0.75 -1.5 4,379,442 + -1.5 4,379,455 + +0.0 4,379,456 - +0.2 4,379,456 - -1.5 4,379,456 - +0.6 4,379,458 - -4.2 4,379,488 + +0.0 4,379,500 + -2.1 4,379,501 - +0.6 4,379,505 - -1.5 4,379,595 - BT3400 0.24 +0.1 4,379,601 + BT3400 0.26 +0.5 4,379,745 - BT3400 0.62 -0.1 4,379,745 - BT3400 0.62 -1.4 4,379,747 - BT3400 0.63 -0.6 4,379,758 + BT3400 0.65 -0.5 4,379,794 - BT3400 0.74 -0.2 4,379,895 - +0.7 4,379,904 - -0.8 4,379,930 - -0.4 4,380,039 - -2.0 4,380,039 - -1.7 4,380,039 - +1.6 4,380,043 + -1.6 4,380,043 + +1.6 4,380,043 + +0.0 4,380,071 + +0.0 4,380,072 - -0.5 4,380,091 + -0.4 4,380,091 + +0.6 4,380,092 - -1.2 4,380,092 - -2.3 4,380,092 - -0.7 4,380,092 - -2.7 4,380,092 - -0.3 4,380,109 + -2.2 4,380,110 - -2.4 4,380,110 - -1.2 4,380,122 - -2.2 4,380,163 + -1.8 4,380,163 + -1.5 4,380,180 + +0.2 4,380,188 + +0.1 4,380,192 - -0.1 4,380,192 - +0.0 4,380,203 + +0.4 4,380,215 + -1.2 4,380,269 + BT3401 0.11 -1.0 4,380,299 + BT3401 0.13 +1.6 4,380,305 + BT3401 0.14 +0.0 4,380,306 - BT3401 0.14 +1.6 4,380,306 - BT3401 0.14 -2.5 4,380,306 - BT3401 0.14 -1.2 4,380,311 - BT3401 0.14 +0.6 4,380,329 + BT3401 0.16 +0.6 4,380,387 + BT3401 0.21 -0.4 4,380,387 + BT3401 0.21 -2.6 4,380,392 + BT3401 0.21 +0.6 4,380,431 - BT3401 0.25 +2.1 4,380,518 + BT3401 0.32 +1.3 4,380,518 + BT3401 0.32 +1.6 4,380,611 - BT3401 0.40 -0.7 4,380,649 - BT3401 0.44 -0.9 4,380,660 - BT3401 0.45 +1.6 4,380,664 - BT3401 0.45 -2.0 4,380,700 - BT3401 0.48 -1.6 4,380,702 - BT3401 0.48 +1.2 4,380,771 - BT3401 0.54 -0.1 4,380,819 - BT3401 0.59 +0.6 4,380,826 + BT3401 0.59 +2.2 4,380,858 - BT3401 0.62 +0.8 4,380,917 + BT3401 0.67 -1.0 4,380,940 - BT3401 0.69 -1.0 4,380,958 - BT3401 0.71 -1.0 4,380,987 + BT3401 0.73 +0.1 4,381,009 - BT3401 0.75 -0.0 4,381,066 - BT3401 0.80 +0.6 4,381,167 - BT3401 0.89 -2.5 4,381,291 - -1.8 4,381,291 - -2.8 4,381,294 + +0.3 4,381,294 + -0.7 4,381,295 - -0.1 4,381,313 + -0.4 4,381,314 - -1.7 4,381,315 + -1.2 4,381,316 - -2.5 4,381,332 - +0.0
Or see this region's nucleotide sequence