Experiment: BHIS with Deoxycholic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3257 and BT_3258 overlap by 4 nucleotides BT_3258 and BT3259 are separated by 107 nucleotides BT3259 and BT3260 are separated by 332 nucleotides
BT3257: BT3257 - putative two-component system sensor histidine kinase (NCBI ptt file), at 4,158,388 to 4,160,466
BT3257
BT_3258: BT_3258 - two-component system response regulator (NCBI), at 4,160,463 to 4,161,242
_3258
BT3259: BT3259 - hypothetical protein (NCBI ptt file), at 4,161,350 to 4,161,745
BT3259
BT3260: BT3260 - DNA polymerase I (NCBI ptt file), at 4,162,078 to 4,164,927
BT3260
Position (kb)
4161
4162 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4160.355 kb on + strand at 4160.356 kb on - strand at 4160.378 kb on + strand at 4160.378 kb on + strand at 4160.434 kb on + strand at 4160.434 kb on + strand at 4160.438 kb on + strand at 4160.670 kb on - strand, within BT_3258 at 4160.670 kb on - strand, within BT_3258 at 4160.670 kb on - strand, within BT_3258 at 4160.726 kb on + strand, within BT_3258 at 4160.738 kb on + strand, within BT_3258 at 4160.738 kb on + strand, within BT_3258 at 4160.742 kb on + strand, within BT_3258 at 4160.842 kb on + strand, within BT_3258 at 4160.843 kb on - strand, within BT_3258 at 4160.918 kb on + strand, within BT_3258 at 4160.918 kb on + strand, within BT_3258 at 4160.919 kb on - strand, within BT_3258 at 4161.007 kb on + strand, within BT_3258 at 4161.007 kb on + strand, within BT_3258 at 4161.008 kb on - strand, within BT_3258 at 4161.047 kb on + strand, within BT_3258 at 4161.086 kb on + strand, within BT_3258 at 4161.176 kb on + strand at 4161.254 kb on + strand at 4161.254 kb on + strand at 4161.259 kb on + strand at 4161.259 kb on + strand at 4161.308 kb on + strand at 4161.309 kb on - strand at 4161.309 kb on - strand at 4161.329 kb on + strand at 4161.329 kb on + strand at 4161.352 kb on - strand at 4161.359 kb on - strand at 4161.361 kb on + strand at 4161.400 kb on - strand, within BT3259 at 4161.425 kb on - strand, within BT3259 at 4161.456 kb on + strand, within BT3259 at 4161.460 kb on + strand, within BT3259 at 4161.461 kb on - strand, within BT3259 at 4161.461 kb on - strand, within BT3259 at 4161.550 kb on - strand, within BT3259 at 4161.553 kb on + strand, within BT3259 at 4161.555 kb on + strand, within BT3259 at 4161.561 kb on + strand, within BT3259 at 4161.568 kb on + strand, within BT3259 at 4161.584 kb on - strand, within BT3259 at 4161.584 kb on - strand, within BT3259 at 4161.584 kb on - strand, within BT3259 at 4161.614 kb on + strand, within BT3259 at 4161.643 kb on + strand, within BT3259 at 4161.643 kb on + strand, within BT3259 at 4161.645 kb on + strand at 4161.663 kb on + strand, within BT3259 at 4161.664 kb on - strand, within BT3259 at 4161.704 kb on + strand, within BT3259 at 4161.711 kb on - strand at 4161.759 kb on + strand at 4161.795 kb on + strand at 4161.795 kb on + strand at 4161.796 kb on - strand at 4161.831 kb on - strand at 4161.847 kb on - strand at 4162.044 kb on + strand at 4162.067 kb on + strand at 4162.075 kb on - strand at 4162.080 kb on - strand at 4162.178 kb on - strand at 4162.178 kb on - strand at 4162.226 kb on - strand at 4162.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Deoxycholic 0.2 mg/ml remove 4,160,355 + +0.1 4,160,356 - +0.4 4,160,378 + -0.9 4,160,378 + -3.3 4,160,434 + -2.7 4,160,434 + -1.3 4,160,438 + -1.5 4,160,670 - BT_3258 0.27 +0.4 4,160,670 - BT_3258 0.27 -2.3 4,160,670 - BT_3258 0.27 -0.2 4,160,726 + BT_3258 0.34 -0.7 4,160,738 + BT_3258 0.35 -1.7 4,160,738 + BT_3258 0.35 -0.6 4,160,742 + BT_3258 0.36 -2.1 4,160,842 + BT_3258 0.49 -0.7 4,160,843 - BT_3258 0.49 -2.1 4,160,918 + BT_3258 0.58 +0.5 4,160,918 + BT_3258 0.58 -2.5 4,160,919 - BT_3258 0.58 -1.0 4,161,007 + BT_3258 0.70 -2.2 4,161,007 + BT_3258 0.70 -0.7 4,161,008 - BT_3258 0.70 -3.5 4,161,047 + BT_3258 0.75 -1.3 4,161,086 + BT_3258 0.80 -3.5 4,161,176 + +0.9 4,161,254 + -1.7 4,161,254 + -0.2 4,161,259 + -2.0 4,161,259 + -2.2 4,161,308 + +1.0 4,161,309 - +0.4 4,161,309 - -0.7 4,161,329 + -0.9 4,161,329 + +0.2 4,161,352 - -1.6 4,161,359 - -1.1 4,161,361 + +0.4 4,161,400 - BT3259 0.13 -0.7 4,161,425 - BT3259 0.19 +0.1 4,161,456 + BT3259 0.27 +0.1 4,161,460 + BT3259 0.28 +1.6 4,161,461 - BT3259 0.28 +0.4 4,161,461 - BT3259 0.28 +0.1 4,161,550 - BT3259 0.51 -0.9 4,161,553 + BT3259 0.51 -0.2 4,161,555 + BT3259 0.52 +1.9 4,161,561 + BT3259 0.53 -0.0 4,161,568 + BT3259 0.55 -0.9 4,161,584 - BT3259 0.59 +0.5 4,161,584 - BT3259 0.59 -1.8 4,161,584 - BT3259 0.59 -0.6 4,161,614 + BT3259 0.67 -2.2 4,161,643 + BT3259 0.74 +0.9 4,161,643 + BT3259 0.74 -2.3 4,161,645 + +1.7 4,161,663 + BT3259 0.79 -2.7 4,161,664 - BT3259 0.79 -1.5 4,161,704 + BT3259 0.89 +0.9 4,161,711 - -1.6 4,161,759 + -1.8 4,161,795 + +0.9 4,161,795 + +0.3 4,161,796 - -2.4 4,161,831 - -1.0 4,161,847 - -2.3 4,162,044 + -1.1 4,162,067 + -0.7 4,162,075 - -1.3 4,162,080 - +0.7 4,162,178 - -3.0 4,162,178 - -3.9 4,162,226 - -1.0 4,162,250 - -1.1
Or see this region's nucleotide sequence