Experiment: BHIS with Deoxycholic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2603 and BT2604 overlap by 4 nucleotides BT2604 and BT2605 are separated by 82 nucleotides BT2605 and BT2606 are separated by 50 nucleotides
BT2603: BT2603 - conserved protein found in conjugate transposon (NCBI ptt file), at 3,243,651 to 3,246,050
BT2603
BT2604: BT2604 - conserved protein found in conjugate transposon (NCBI ptt file), at 3,246,047 to 3,246,754
BT2604
BT2605: BT2605 - hypothetical protein (NCBI ptt file), at 3,246,837 to 3,247,034
BT2605
BT2606: BT2606 - conserved hypothetical protein (NCBI ptt file), at 3,247,085 to 3,248,092
BT2606
Position (kb)
3246
3247 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3245.088 kb on + strand, within BT2603 at 3245.159 kb on - strand, within BT2603 at 3245.228 kb on + strand, within BT2603 at 3245.236 kb on + strand, within BT2603 at 3245.249 kb on + strand, within BT2603 at 3245.250 kb on - strand, within BT2603 at 3245.321 kb on + strand, within BT2603 at 3245.321 kb on + strand, within BT2603 at 3245.322 kb on - strand, within BT2603 at 3245.336 kb on + strand, within BT2603 at 3245.382 kb on - strand, within BT2603 at 3245.571 kb on - strand, within BT2603 at 3245.710 kb on + strand, within BT2603 at 3245.717 kb on - strand, within BT2603 at 3245.809 kb on + strand, within BT2603 at 3245.922 kb on - strand at 3246.014 kb on + strand at 3246.014 kb on + strand at 3246.014 kb on + strand at 3246.015 kb on - strand at 3246.040 kb on - strand at 3246.080 kb on + strand at 3246.242 kb on + strand, within BT2604 at 3246.242 kb on + strand, within BT2604 at 3246.244 kb on + strand, within BT2604 at 3246.247 kb on - strand, within BT2604 at 3246.485 kb on + strand, within BT2604 at 3246.672 kb on + strand, within BT2604 at 3246.694 kb on + strand at 3246.716 kb on + strand at 3246.716 kb on + strand at 3246.741 kb on + strand at 3246.741 kb on + strand at 3246.784 kb on + strand at 3246.784 kb on + strand at 3246.784 kb on + strand at 3246.789 kb on + strand at 3246.858 kb on + strand, within BT2605 at 3246.864 kb on + strand, within BT2605 at 3246.937 kb on - strand, within BT2605 at 3247.050 kb on - strand at 3247.100 kb on + strand at 3247.107 kb on - strand at 3247.194 kb on + strand, within BT2606 at 3247.195 kb on - strand, within BT2606 at 3247.356 kb on - strand, within BT2606 at 3247.454 kb on + strand, within BT2606 at 3247.513 kb on + strand, within BT2606 at 3247.513 kb on + strand, within BT2606 at 3247.562 kb on - strand, within BT2606 at 3247.627 kb on + strand, within BT2606 at 3247.698 kb on + strand, within BT2606
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Deoxycholic 0.2 mg/ml remove 3,245,088 + BT2603 0.60 +0.7 3,245,159 - BT2603 0.63 -3.1 3,245,228 + BT2603 0.66 +0.5 3,245,236 + BT2603 0.66 +1.2 3,245,249 + BT2603 0.67 -1.7 3,245,250 - BT2603 0.67 +0.1 3,245,321 + BT2603 0.70 -0.3 3,245,321 + BT2603 0.70 -1.3 3,245,322 - BT2603 0.70 +0.3 3,245,336 + BT2603 0.70 +1.1 3,245,382 - BT2603 0.72 -1.3 3,245,571 - BT2603 0.80 +0.2 3,245,710 + BT2603 0.86 -0.4 3,245,717 - BT2603 0.86 -1.5 3,245,809 + BT2603 0.90 -2.7 3,245,922 - -0.9 3,246,014 + -2.7 3,246,014 + -0.7 3,246,014 + +0.6 3,246,015 - +0.1 3,246,040 - -1.5 3,246,080 + -0.7 3,246,242 + BT2604 0.28 -0.7 3,246,242 + BT2604 0.28 -0.9 3,246,244 + BT2604 0.28 -1.9 3,246,247 - BT2604 0.28 -0.7 3,246,485 + BT2604 0.62 -1.5 3,246,672 + BT2604 0.88 -1.4 3,246,694 + -0.2 3,246,716 + -1.2 3,246,716 + -1.1 3,246,741 + -1.2 3,246,741 + -0.7 3,246,784 + -0.4 3,246,784 + -0.9 3,246,784 + +0.5 3,246,789 + -0.7 3,246,858 + BT2605 0.11 +0.5 3,246,864 + BT2605 0.14 -2.6 3,246,937 - BT2605 0.51 +1.6 3,247,050 - -0.5 3,247,100 + -0.5 3,247,107 - +0.1 3,247,194 + BT2606 0.11 -1.8 3,247,195 - BT2606 0.11 -1.9 3,247,356 - BT2606 0.27 -2.1 3,247,454 + BT2606 0.37 -1.5 3,247,513 + BT2606 0.42 -1.5 3,247,513 + BT2606 0.42 -0.7 3,247,562 - BT2606 0.47 -1.2 3,247,627 + BT2606 0.54 -0.4 3,247,698 + BT2606 0.61 -0.6
Or see this region's nucleotide sequence