Experiment: BHIS with Deoxycholic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2564 and BT2565 are separated by 180 nucleotides BT2565 and BT2566 are separated by 52 nucleotides BT2566 and BT2567 are separated by 76 nucleotides
BT2564: BT2564 - conserved hypothetical protein (NCBI ptt file), at 3,202,032 to 3,203,183
BT2564
BT2565: BT2565 - transcription elongation factor greA (NCBI ptt file), at 3,203,364 to 3,203,828
BT2565
BT2566: BT2566 - HIT family protein (NCBI ptt file), at 3,203,881 to 3,204,273
BT2566
BT2567: BT2567 - putative permease (NCBI ptt file), at 3,204,350 to 3,205,270
BT2567
Position (kb)
3203
3204
3205 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3202.910 kb on + strand, within BT2564 at 3202.911 kb on - strand, within BT2564 at 3202.928 kb on - strand, within BT2564 at 3202.928 kb on - strand, within BT2564 at 3202.929 kb on + strand, within BT2564 at 3202.931 kb on + strand, within BT2564 at 3202.932 kb on - strand, within BT2564 at 3202.938 kb on + strand, within BT2564 at 3203.032 kb on + strand, within BT2564 at 3203.033 kb on - strand, within BT2564 at 3203.066 kb on - strand, within BT2564 at 3203.072 kb on - strand at 3203.073 kb on + strand at 3203.074 kb on - strand at 3203.074 kb on - strand at 3203.075 kb on + strand at 3203.075 kb on + strand at 3203.076 kb on - strand at 3203.076 kb on - strand at 3203.181 kb on + strand at 3203.220 kb on - strand at 3203.223 kb on + strand at 3203.247 kb on + strand at 3203.330 kb on + strand at 3203.330 kb on + strand at 3203.330 kb on + strand at 3203.818 kb on + strand at 3203.818 kb on + strand at 3203.867 kb on - strand at 3203.870 kb on + strand at 3203.891 kb on + strand at 3203.892 kb on - strand at 3203.899 kb on - strand at 3203.899 kb on - strand at 3203.927 kb on - strand, within BT2566 at 3203.991 kb on + strand, within BT2566 at 3203.992 kb on - strand, within BT2566 at 3203.992 kb on - strand, within BT2566 at 3203.992 kb on - strand, within BT2566 at 3204.022 kb on + strand, within BT2566 at 3204.080 kb on + strand, within BT2566 at 3204.080 kb on + strand, within BT2566 at 3204.175 kb on - strand, within BT2566 at 3204.192 kb on + strand, within BT2566 at 3204.193 kb on - strand, within BT2566 at 3204.271 kb on + strand at 3204.289 kb on + strand at 3204.290 kb on - strand at 3204.290 kb on - strand at 3204.336 kb on + strand at 3204.337 kb on - strand at 3204.337 kb on - strand at 3204.341 kb on + strand at 3204.343 kb on + strand at 3204.343 kb on + strand at 3204.344 kb on - strand at 3204.352 kb on - strand at 3204.451 kb on + strand, within BT2567 at 3204.541 kb on - strand, within BT2567 at 3204.565 kb on + strand, within BT2567 at 3204.565 kb on + strand, within BT2567 at 3204.565 kb on + strand, within BT2567 at 3204.565 kb on + strand, within BT2567 at 3204.567 kb on + strand, within BT2567 at 3204.568 kb on - strand, within BT2567 at 3204.587 kb on + strand, within BT2567 at 3204.588 kb on - strand, within BT2567 at 3204.588 kb on - strand, within BT2567 at 3204.634 kb on + strand, within BT2567 at 3204.634 kb on + strand, within BT2567 at 3204.664 kb on + strand, within BT2567 at 3204.676 kb on + strand, within BT2567 at 3204.677 kb on - strand, within BT2567 at 3204.679 kb on + strand, within BT2567 at 3204.707 kb on - strand, within BT2567 at 3204.707 kb on - strand, within BT2567 at 3204.719 kb on - strand, within BT2567 at 3204.719 kb on - strand, within BT2567 at 3204.745 kb on + strand, within BT2567 at 3204.746 kb on - strand, within BT2567 at 3204.765 kb on + strand, within BT2567 at 3204.766 kb on - strand, within BT2567 at 3204.766 kb on - strand, within BT2567 at 3204.952 kb on + strand, within BT2567 at 3204.971 kb on + strand, within BT2567 at 3204.984 kb on - strand, within BT2567 at 3205.024 kb on + strand, within BT2567 at 3205.024 kb on + strand, within BT2567 at 3205.025 kb on - strand, within BT2567 at 3205.188 kb on + strand at 3205.188 kb on + strand at 3205.238 kb on - strand at 3205.261 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Deoxycholic 0.2 mg/ml remove 3,202,910 + BT2564 0.76 -0.2 3,202,911 - BT2564 0.76 -1.8 3,202,928 - BT2564 0.78 -0.2 3,202,928 - BT2564 0.78 -0.2 3,202,929 + BT2564 0.78 -1.2 3,202,931 + BT2564 0.78 -1.8 3,202,932 - BT2564 0.78 +0.0 3,202,938 + BT2564 0.79 -1.0 3,203,032 + BT2564 0.87 -0.4 3,203,033 - BT2564 0.87 -1.1 3,203,066 - BT2564 0.90 +0.6 3,203,072 - -1.0 3,203,073 + -2.8 3,203,074 - -0.7 3,203,074 - -0.3 3,203,075 + -1.2 3,203,075 + +0.0 3,203,076 - -2.2 3,203,076 - -1.8 3,203,181 + -2.0 3,203,220 - +0.5 3,203,223 + +1.6 3,203,247 + +0.6 3,203,330 + +0.0 3,203,330 + -2.1 3,203,330 + -2.2 3,203,818 + +1.6 3,203,818 + -2.8 3,203,867 - -1.9 3,203,870 + +1.2 3,203,891 + -1.8 3,203,892 - -1.5 3,203,899 - +1.6 3,203,899 - -0.3 3,203,927 - BT2566 0.12 -2.2 3,203,991 + BT2566 0.28 -2.5 3,203,992 - BT2566 0.28 -1.1 3,203,992 - BT2566 0.28 -1.0 3,203,992 - BT2566 0.28 -0.5 3,204,022 + BT2566 0.36 -2.3 3,204,080 + BT2566 0.51 -1.6 3,204,080 + BT2566 0.51 -1.5 3,204,175 - BT2566 0.75 -1.4 3,204,192 + BT2566 0.79 -1.4 3,204,193 - BT2566 0.79 -1.0 3,204,271 + +0.3 3,204,289 + -0.7 3,204,290 - -2.8 3,204,290 - -0.4 3,204,336 + -4.3 3,204,337 - -0.2 3,204,337 - +0.3 3,204,341 + +0.6 3,204,343 + -1.0 3,204,343 + -2.1 3,204,344 - +0.3 3,204,352 - +0.3 3,204,451 + BT2567 0.11 -3.0 3,204,541 - BT2567 0.21 -0.3 3,204,565 + BT2567 0.23 -3.1 3,204,565 + BT2567 0.23 -2.7 3,204,565 + BT2567 0.23 +0.6 3,204,565 + BT2567 0.23 -1.4 3,204,567 + BT2567 0.24 -0.8 3,204,568 - BT2567 0.24 -3.0 3,204,587 + BT2567 0.26 -2.1 3,204,588 - BT2567 0.26 -0.7 3,204,588 - BT2567 0.26 +0.4 3,204,634 + BT2567 0.31 +0.3 3,204,634 + BT2567 0.31 -0.4 3,204,664 + BT2567 0.34 -1.4 3,204,676 + BT2567 0.35 +0.0 3,204,677 - BT2567 0.36 -2.3 3,204,679 + BT2567 0.36 +3.2 3,204,707 - BT2567 0.39 -1.0 3,204,707 - BT2567 0.39 -0.2 3,204,719 - BT2567 0.40 +0.3 3,204,719 - BT2567 0.40 -2.0 3,204,745 + BT2567 0.43 -0.6 3,204,746 - BT2567 0.43 -0.4 3,204,765 + BT2567 0.45 -0.0 3,204,766 - BT2567 0.45 -1.4 3,204,766 - BT2567 0.45 -0.4 3,204,952 + BT2567 0.65 -1.4 3,204,971 + BT2567 0.67 -1.0 3,204,984 - BT2567 0.69 +0.0 3,205,024 + BT2567 0.73 -2.6 3,205,024 + BT2567 0.73 -2.1 3,205,025 - BT2567 0.73 -1.4 3,205,188 + +0.2 3,205,188 + +0.6 3,205,238 - -2.3 3,205,261 + -1.2
Or see this region's nucleotide sequence