Experiment: BHIS with Deoxycholic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0520 and BT0521 are separated by 583 nucleotides BT0521 and BT0522 overlap by 19 nucleotides BT0522 and BT0523 are separated by 15 nucleotides
BT0520: BT0520 - transcriptional regulator (NCBI ptt file), at 642,518 to 643,129
BT0520
BT0521: BT0521 - putative acetyl transferase (NCBI ptt file), at 643,713 to 644,336
BT0521
BT0522: BT0522 - hypothetical protein (NCBI ptt file), at 644,318 to 645,232
BT0522
BT0523: BT0523 - putative glycosyltransferase (NCBI ptt file), at 645,248 to 646,408
BT0523
Position (kb) 643
644
645 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 642.724 kb on - strand, within BT0520 at 642.812 kb on + strand, within BT0520 at 643.034 kb on - strand, within BT0520 at 643.079 kb on + strand at 643.093 kb on + strand at 643.244 kb on - strand at 643.328 kb on + strand at 643.333 kb on - strand at 643.457 kb on + strand at 643.482 kb on - strand at 643.485 kb on + strand at 643.486 kb on - strand at 643.486 kb on - strand at 643.486 kb on - strand at 643.501 kb on + strand at 643.619 kb on + strand at 643.662 kb on + strand at 643.664 kb on + strand at 643.665 kb on - strand at 643.665 kb on - strand at 643.665 kb on - strand at 643.670 kb on - strand at 643.671 kb on + strand at 643.692 kb on - strand at 643.834 kb on - strand, within BT0521 at 643.848 kb on + strand, within BT0521 at 643.867 kb on - strand, within BT0521 at 643.924 kb on - strand at 643.970 kb on + strand, within BT0521 at 643.989 kb on - strand, within BT0521 at 644.040 kb on - strand at 644.063 kb on - strand, within BT0521 at 644.093 kb on + strand, within BT0521 at 644.094 kb on - strand, within BT0521 at 644.094 kb on - strand, within BT0521 at 644.116 kb on - strand, within BT0521 at 644.210 kb on - strand, within BT0521 at 644.212 kb on - strand, within BT0521 at 644.510 kb on + strand, within BT0522 at 644.556 kb on - strand, within BT0522 at 644.570 kb on + strand, within BT0522 at 644.571 kb on + strand, within BT0522 at 644.611 kb on - strand, within BT0522 at 644.634 kb on - strand, within BT0522 at 644.634 kb on - strand, within BT0522 at 644.659 kb on + strand, within BT0522 at 644.660 kb on - strand, within BT0522 at 644.660 kb on - strand, within BT0522 at 644.661 kb on + strand, within BT0522 at 644.666 kb on - strand, within BT0522 at 644.810 kb on - strand, within BT0522 at 644.863 kb on + strand, within BT0522 at 644.864 kb on - strand, within BT0522 at 644.877 kb on - strand, within BT0522 at 644.905 kb on - strand, within BT0522 at 644.973 kb on - strand, within BT0522 at 644.973 kb on - strand, within BT0522 at 644.994 kb on - strand, within BT0522 at 645.054 kb on + strand, within BT0522 at 645.056 kb on + strand, within BT0522 at 645.086 kb on + strand, within BT0522 at 645.086 kb on + strand, within BT0522 at 645.086 kb on + strand, within BT0522 at 645.212 kb on + strand at 645.213 kb on - strand at 645.217 kb on - strand at 645.217 kb on - strand at 645.220 kb on + strand at 645.277 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Deoxycholic 0.2 mg/ml remove 642,724 - BT0520 0.34 +0.2 642,812 + BT0520 0.48 -1.4 643,034 - BT0520 0.84 -1.2 643,079 + +0.0 643,093 + +0.6 643,244 - +2.1 643,328 + -1.2 643,333 - -0.0 643,457 + +1.3 643,482 - -1.0 643,485 + +0.8 643,486 - -2.8 643,486 - +1.6 643,486 - -0.3 643,501 + +0.6 643,619 + +1.6 643,662 + -0.0 643,664 + -2.7 643,665 - -1.6 643,665 - -0.8 643,665 - +1.6 643,670 - -0.8 643,671 + -0.0 643,692 - -1.1 643,834 - BT0521 0.19 +1.2 643,848 + BT0521 0.22 +0.3 643,867 - BT0521 0.25 -1.4 643,924 - -1.0 643,970 + BT0521 0.41 -0.4 643,989 - BT0521 0.44 -0.4 644,040 - -0.4 644,063 - BT0521 0.56 -0.2 644,093 + BT0521 0.61 -1.5 644,094 - BT0521 0.61 -0.8 644,094 - BT0521 0.61 +2.3 644,116 - BT0521 0.65 -1.0 644,210 - BT0521 0.80 -0.2 644,212 - BT0521 0.80 +0.7 644,510 + BT0522 0.21 -1.6 644,556 - BT0522 0.26 -0.7 644,570 + BT0522 0.28 -0.0 644,571 + BT0522 0.28 +1.0 644,611 - BT0522 0.32 -0.0 644,634 - BT0522 0.35 -0.0 644,634 - BT0522 0.35 +1.6 644,659 + BT0522 0.37 +0.7 644,660 - BT0522 0.37 +0.2 644,660 - BT0522 0.37 -0.4 644,661 + BT0522 0.37 -0.4 644,666 - BT0522 0.38 -0.0 644,810 - BT0522 0.54 -0.0 644,863 + BT0522 0.60 -1.0 644,864 - BT0522 0.60 -1.2 644,877 - BT0522 0.61 -0.9 644,905 - BT0522 0.64 -0.0 644,973 - BT0522 0.72 -1.0 644,973 - BT0522 0.72 -0.6 644,994 - BT0522 0.74 -0.8 645,054 + BT0522 0.80 -4.7 645,056 + BT0522 0.81 +0.6 645,086 + BT0522 0.84 -2.8 645,086 + BT0522 0.84 -1.0 645,086 + BT0522 0.84 -0.4 645,212 + -2.1 645,213 - -0.4 645,217 - -0.0 645,217 - -0.4 645,220 + -3.5 645,277 + -0.2
Or see this region's nucleotide sequence