Experiment: BHIS with Deoxycholic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0281 and BT0282 are separated by 301 nucleotides BT0282 and BT0283 are separated by 15 nucleotides BT0283 and BT0284 are separated by 45 nucleotides
BT0281: BT0281 - putative DNA-binding protein (NCBI ptt file), at 326,008 to 326,649
BT0281
BT0282: BT0282 - conserved hypothetical protein (NCBI ptt file), at 326,951 to 328,246
BT0282
BT0283: BT0283 - hypothetical protein (NCBI ptt file), at 328,262 to 328,447
BT0283
BT0284: BT0284 - putative peptidoglycan binding protein (LPXTG motif) (NCBI ptt file), at 328,493 to 329,944
BT0284
Position (kb) 326
327
328
329 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 326.039 kb on - strand at 326.066 kb on + strand at 326.067 kb on - strand at 326.068 kb on + strand at 326.208 kb on - strand, within BT0281 at 326.314 kb on - strand, within BT0281 at 326.323 kb on - strand, within BT0281 at 326.330 kb on - strand, within BT0281 at 326.330 kb on - strand, within BT0281 at 326.331 kb on + strand, within BT0281 at 326.332 kb on - strand, within BT0281 at 326.417 kb on - strand, within BT0281 at 326.626 kb on - strand at 326.627 kb on + strand at 326.849 kb on - strand at 326.917 kb on - strand at 327.046 kb on - strand at 327.061 kb on + strand at 327.062 kb on - strand at 327.461 kb on - strand, within BT0282 at 327.476 kb on - strand, within BT0282 at 327.555 kb on - strand, within BT0282 at 327.558 kb on + strand, within BT0282 at 327.559 kb on - strand, within BT0282 at 327.646 kb on - strand, within BT0282 at 327.767 kb on + strand, within BT0282 at 327.798 kb on + strand, within BT0282 at 327.799 kb on - strand, within BT0282 at 327.866 kb on - strand, within BT0282 at 327.911 kb on + strand, within BT0282 at 327.912 kb on - strand, within BT0282 at 328.016 kb on + strand, within BT0282 at 328.176 kb on + strand at 328.177 kb on - strand at 328.208 kb on - strand at 328.208 kb on - strand at 328.451 kb on - strand at 328.555 kb on - strand at 328.573 kb on + strand at 328.635 kb on + strand at 328.635 kb on + strand at 328.705 kb on - strand, within BT0284 at 328.715 kb on - strand, within BT0284 at 328.789 kb on - strand, within BT0284 at 328.822 kb on + strand, within BT0284 at 328.822 kb on + strand, within BT0284 at 328.907 kb on - strand at 328.942 kb on + strand, within BT0284 at 328.943 kb on - strand, within BT0284 at 328.998 kb on + strand, within BT0284 at 328.998 kb on + strand at 329.009 kb on + strand, within BT0284 at 329.022 kb on + strand, within BT0284 at 329.022 kb on + strand, within BT0284 at 329.044 kb on + strand, within BT0284 at 329.125 kb on + strand, within BT0284 at 329.126 kb on - strand, within BT0284 at 329.171 kb on - strand, within BT0284 at 329.173 kb on - strand, within BT0284 at 329.209 kb on + strand, within BT0284
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Deoxycholic 0.2 mg/ml remove 326,039 - +0.5 326,066 + -0.4 326,067 - -0.4 326,068 + -0.5 326,208 - BT0281 0.31 +0.5 326,314 - BT0281 0.48 +1.3 326,323 - BT0281 0.49 +4.1 326,330 - BT0281 0.50 +1.0 326,330 - BT0281 0.50 -0.8 326,331 + BT0281 0.50 -0.8 326,332 - BT0281 0.50 +0.2 326,417 - BT0281 0.64 -0.9 326,626 - -0.8 326,627 + -1.3 326,849 - +2.5 326,917 - -0.5 327,046 - +0.8 327,061 + +0.3 327,062 - -0.8 327,461 - BT0282 0.39 -3.2 327,476 - BT0282 0.41 -2.2 327,555 - BT0282 0.47 -0.0 327,558 + BT0282 0.47 -2.1 327,559 - BT0282 0.47 -0.5 327,646 - BT0282 0.54 -0.5 327,767 + BT0282 0.63 -0.8 327,798 + BT0282 0.65 -1.0 327,799 - BT0282 0.65 -2.0 327,866 - BT0282 0.71 +0.5 327,911 + BT0282 0.74 -1.3 327,912 - BT0282 0.74 -0.5 328,016 + BT0282 0.82 -1.8 328,176 + -2.5 328,177 - -1.9 328,208 - -0.0 328,208 - +2.1 328,451 - +0.3 328,555 - -1.4 328,573 + -0.2 328,635 + -1.3 328,635 + +0.5 328,705 - BT0284 0.15 -0.5 328,715 - BT0284 0.15 +0.2 328,789 - BT0284 0.20 -0.9 328,822 + BT0284 0.23 -0.0 328,822 + BT0284 0.23 -1.3 328,907 - +1.0 328,942 + BT0284 0.31 -3.4 328,943 - BT0284 0.31 +3.1 328,998 + BT0284 0.35 -1.5 328,998 + -0.0 329,009 + BT0284 0.36 -1.5 329,022 + BT0284 0.36 -1.3 329,022 + BT0284 0.36 +0.1 329,044 + BT0284 0.38 -1.7 329,125 + BT0284 0.44 +0.5 329,126 - BT0284 0.44 +0.4 329,171 - BT0284 0.47 +0.5 329,173 - BT0284 0.47 -1.2 329,209 + BT0284 0.49 -3.4
Or see this region's nucleotide sequence