Experiment: KB with Khayasin C 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3979 and Psyr_3980 are separated by 41 nucleotides Psyr_3980 and Psyr_3981 are separated by 35 nucleotides Psyr_3981 and Psyr_3982 are separated by 126 nucleotides
Psyr_3979: Psyr_3979 - conserved hypothetical protein, at 4,730,539 to 4,733,874
_3979
Psyr_3980: Psyr_3980 - hypothetical protein, at 4,733,916 to 4,734,152
_3980
Psyr_3981: Psyr_3981 - ribosomal small subunit pseudouridine synthase A, at 4,734,188 to 4,734,880
_3981
Psyr_3982: Psyr_3982 - 3-hydroxyacyl-CoA-acyl carrier protein transferase, at 4,735,007 to 4,735,888
_3982
Position (kb)
4734
4735 Strain fitness (log2 ratio)
-1
0
1
2 at 4733.253 kb on + strand, within Psyr_3979 at 4733.254 kb on - strand, within Psyr_3979 at 4733.267 kb on + strand, within Psyr_3979 at 4733.268 kb on - strand, within Psyr_3979 at 4733.323 kb on + strand, within Psyr_3979 at 4733.323 kb on + strand, within Psyr_3979 at 4733.323 kb on + strand, within Psyr_3979 at 4733.323 kb on + strand, within Psyr_3979 at 4733.324 kb on - strand, within Psyr_3979 at 4733.324 kb on - strand, within Psyr_3979 at 4733.324 kb on - strand, within Psyr_3979 at 4733.324 kb on - strand, within Psyr_3979 at 4733.324 kb on - strand, within Psyr_3979 at 4733.392 kb on + strand, within Psyr_3979 at 4733.392 kb on + strand, within Psyr_3979 at 4733.430 kb on + strand, within Psyr_3979 at 4733.560 kb on + strand at 4733.561 kb on - strand at 4733.561 kb on - strand at 4733.561 kb on - strand at 4733.561 kb on - strand at 4733.561 kb on - strand at 4733.599 kb on + strand at 4733.599 kb on + strand at 4733.599 kb on + strand at 4733.599 kb on + strand at 4733.599 kb on + strand at 4733.599 kb on + strand at 4733.600 kb on - strand at 4733.771 kb on + strand at 4733.771 kb on + strand at 4733.771 kb on + strand at 4733.830 kb on + strand at 4733.830 kb on + strand at 4733.830 kb on + strand at 4733.830 kb on + strand at 4733.830 kb on + strand at 4733.830 kb on + strand at 4733.831 kb on - strand at 4733.862 kb on + strand at 4733.863 kb on - strand at 4733.863 kb on - strand at 4733.863 kb on - strand at 4733.863 kb on - strand at 4733.863 kb on - strand at 4733.865 kb on + strand at 4733.865 kb on + strand at 4733.865 kb on + strand at 4733.906 kb on + strand at 4734.150 kb on + strand at 4734.150 kb on + strand at 4734.150 kb on + strand at 4734.151 kb on - strand at 4734.151 kb on - strand at 4734.151 kb on - strand at 4734.151 kb on - strand at 4734.151 kb on - strand at 4734.151 kb on - strand at 4734.273 kb on + strand, within Psyr_3981 at 4734.371 kb on + strand, within Psyr_3981 at 4734.372 kb on - strand, within Psyr_3981 at 4734.581 kb on + strand, within Psyr_3981 at 4734.582 kb on - strand, within Psyr_3981 at 4734.582 kb on - strand, within Psyr_3981 at 4734.582 kb on - strand, within Psyr_3981 at 4734.582 kb on - strand, within Psyr_3981 at 4734.584 kb on + strand, within Psyr_3981 at 4734.585 kb on - strand, within Psyr_3981 at 4734.585 kb on - strand, within Psyr_3981 at 4734.645 kb on - strand, within Psyr_3981 at 4734.691 kb on - strand, within Psyr_3981 at 4734.842 kb on + strand at 4734.843 kb on - strand at 4734.980 kb on + strand at 4734.981 kb on - strand at 4734.981 kb on - strand at 4734.981 kb on - strand at 4734.981 kb on - strand at 4734.981 kb on - strand at 4735.006 kb on + strand at 4735.006 kb on + strand at 4735.006 kb on + strand at 4735.006 kb on + strand at 4735.007 kb on - strand at 4735.007 kb on - strand at 4735.007 kb on - strand at 4735.007 kb on - strand at 4735.007 kb on - strand at 4735.007 kb on - strand at 4735.055 kb on + strand at 4735.056 kb on - strand at 4735.181 kb on + strand, within Psyr_3982 at 4735.194 kb on - strand, within Psyr_3982 at 4735.418 kb on + strand, within Psyr_3982 at 4735.419 kb on - strand, within Psyr_3982 at 4735.419 kb on - strand, within Psyr_3982 at 4735.461 kb on - strand, within Psyr_3982 at 4735.508 kb on - strand, within Psyr_3982 at 4735.537 kb on + strand, within Psyr_3982 at 4735.537 kb on + strand, within Psyr_3982 at 4735.537 kb on + strand, within Psyr_3982 at 4735.562 kb on + strand, within Psyr_3982 at 4735.562 kb on + strand, within Psyr_3982 at 4735.578 kb on - strand, within Psyr_3982 at 4735.616 kb on + strand, within Psyr_3982 at 4735.616 kb on + strand, within Psyr_3982 at 4735.616 kb on + strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Khayasin C 10 mM remove 4,733,253 + Psyr_3979 0.81 +0.9 4,733,254 - Psyr_3979 0.81 +0.9 4,733,267 + Psyr_3979 0.82 -0.3 4,733,268 - Psyr_3979 0.82 +0.3 4,733,323 + Psyr_3979 0.83 -0.7 4,733,323 + Psyr_3979 0.83 -0.0 4,733,323 + Psyr_3979 0.83 -0.2 4,733,323 + Psyr_3979 0.83 -0.2 4,733,324 - Psyr_3979 0.83 -0.2 4,733,324 - Psyr_3979 0.83 +0.5 4,733,324 - Psyr_3979 0.83 +0.9 4,733,324 - Psyr_3979 0.83 -1.2 4,733,324 - Psyr_3979 0.83 +1.6 4,733,392 + Psyr_3979 0.86 -0.3 4,733,392 + Psyr_3979 0.86 +0.6 4,733,430 + Psyr_3979 0.87 -0.5 4,733,560 + -0.0 4,733,561 - -0.6 4,733,561 - +0.3 4,733,561 - +1.8 4,733,561 - -1.5 4,733,561 - -1.5 4,733,599 + +0.1 4,733,599 + +0.1 4,733,599 + +1.0 4,733,599 + -0.7 4,733,599 + +0.4 4,733,599 + +0.7 4,733,600 - +0.1 4,733,771 + -0.4 4,733,771 + -0.1 4,733,771 + +0.3 4,733,830 + -0.1 4,733,830 + +0.4 4,733,830 + -0.9 4,733,830 + -0.3 4,733,830 + +0.2 4,733,830 + +0.2 4,733,831 - +0.5 4,733,862 + +0.4 4,733,863 - +1.0 4,733,863 - +0.5 4,733,863 - +0.8 4,733,863 - +0.6 4,733,863 - +0.2 4,733,865 + -0.1 4,733,865 + -0.1 4,733,865 + -0.2 4,733,906 + +0.7 4,734,150 + +0.5 4,734,150 + +0.9 4,734,150 + +0.9 4,734,151 - +0.5 4,734,151 - -1.0 4,734,151 - +1.0 4,734,151 - +0.4 4,734,151 - -0.3 4,734,151 - -1.7 4,734,273 + Psyr_3981 0.12 -0.2 4,734,371 + Psyr_3981 0.26 -0.5 4,734,372 - Psyr_3981 0.27 +0.2 4,734,581 + Psyr_3981 0.57 -1.3 4,734,582 - Psyr_3981 0.57 +1.4 4,734,582 - Psyr_3981 0.57 +0.6 4,734,582 - Psyr_3981 0.57 +0.6 4,734,582 - Psyr_3981 0.57 -0.3 4,734,584 + Psyr_3981 0.57 -0.5 4,734,585 - Psyr_3981 0.57 +0.2 4,734,585 - Psyr_3981 0.57 +0.3 4,734,645 - Psyr_3981 0.66 +0.2 4,734,691 - Psyr_3981 0.73 +1.9 4,734,842 + -0.3 4,734,843 - +0.6 4,734,980 + +0.3 4,734,981 - +1.3 4,734,981 - -0.5 4,734,981 - +1.0 4,734,981 - +0.9 4,734,981 - -0.6 4,735,006 + +1.3 4,735,006 + +0.6 4,735,006 + +0.9 4,735,006 + -0.3 4,735,007 - +1.0 4,735,007 - -1.0 4,735,007 - -1.0 4,735,007 - +0.6 4,735,007 - +1.0 4,735,007 - +1.3 4,735,055 + -1.2 4,735,056 - -0.5 4,735,181 + Psyr_3982 0.20 -1.1 4,735,194 - Psyr_3982 0.21 -0.6 4,735,418 + Psyr_3982 0.47 +1.3 4,735,419 - Psyr_3982 0.47 +0.0 4,735,419 - Psyr_3982 0.47 -0.1 4,735,461 - Psyr_3982 0.51 -0.6 4,735,508 - Psyr_3982 0.57 +0.6 4,735,537 + Psyr_3982 0.60 +0.2 4,735,537 + Psyr_3982 0.60 +1.8 4,735,537 + Psyr_3982 0.60 -0.0 4,735,562 + Psyr_3982 0.63 -0.9 4,735,562 + Psyr_3982 0.63 -0.8 4,735,578 - Psyr_3982 0.65 -0.4 4,735,616 + Psyr_3982 0.69 -0.3 4,735,616 + Psyr_3982 0.69 -0.4 4,735,616 + Psyr_3982 0.69 +0.9 4,735,617 - Psyr_3982 0.69 -0.7 4,735,617 - Psyr_3982 0.69 +1.4 4,735,617 - Psyr_3982 0.69 -0.0 4,735,617 - Psyr_3982 0.69 +0.4 4,735,617 - Psyr_3982 0.69 -0.0 4,735,617 - Psyr_3982 0.69 +1.3 4,735,679 + Psyr_3982 0.76 +1.0 4,735,679 + Psyr_3982 0.76 -0.5 4,735,679 + Psyr_3982 0.76 +0.9 4,735,679 + Psyr_3982 0.76 +0.5 4,735,679 + Psyr_3982 0.76 -0.7 4,735,680 - Psyr_3982 0.76 +0.6 4,735,680 - Psyr_3982 0.76 -0.6 4,735,680 - Psyr_3982 0.76 -0.7 4,735,680 - Psyr_3982 0.76 +0.2 4,735,680 - Psyr_3982 0.76 +0.6 4,735,680 - Psyr_3982 0.76 +0.3
Or see this region's nucleotide sequence