Experiment: KB with Khayasin C 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2995 and Psyr_2996 are separated by 17 nucleotides Psyr_2996 and Psyr_2997 are separated by 156 nucleotides Psyr_2997 and Psyr_2998 overlap by 4 nucleotides
Psyr_2995: Psyr_2995 - maltooligosyl trehalose synthase, at 3,589,162 to 3,591,945
_2995
Psyr_2996: Psyr_2996 - conserved hypothetical protein, at 3,591,963 to 3,592,364
_2996
Psyr_2997: Psyr_2997 - Glycoside hydrolase, family 13, N-terminal:Alpha amylase, catalytic region, at 3,592,521 to 3,594,704
_2997
Psyr_2998: Psyr_2998 - conserved hypothetical protein, at 3,594,701 to 3,595,714
_2998
Position (kb)
3592
3593
3594
3595 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3591.643 kb on + strand, within Psyr_2995 at 3591.729 kb on - strand at 3591.752 kb on + strand at 3591.752 kb on + strand at 3591.753 kb on - strand at 3591.887 kb on + strand at 3591.930 kb on + strand at 3591.939 kb on + strand at 3591.939 kb on + strand at 3591.939 kb on + strand at 3591.939 kb on + strand at 3591.939 kb on + strand at 3591.939 kb on + strand at 3591.939 kb on + strand at 3591.939 kb on + strand at 3591.940 kb on - strand at 3591.940 kb on - strand at 3591.940 kb on - strand at 3591.940 kb on - strand at 3591.943 kb on + strand at 3591.943 kb on + strand at 3591.943 kb on + strand at 3591.943 kb on + strand at 3591.944 kb on - strand at 3591.944 kb on - strand at 3591.944 kb on - strand at 3591.944 kb on - strand at 3591.944 kb on - strand at 3591.944 kb on - strand at 3591.946 kb on + strand at 3591.946 kb on + strand at 3591.947 kb on - strand at 3591.947 kb on - strand at 3591.947 kb on - strand at 3591.947 kb on - strand at 3592.142 kb on - strand, within Psyr_2996 at 3592.393 kb on - strand at 3592.393 kb on - strand at 3592.429 kb on + strand at 3592.539 kb on - strand at 3592.539 kb on - strand at 3592.553 kb on + strand at 3592.553 kb on + strand at 3592.554 kb on - strand at 3592.685 kb on - strand at 3592.761 kb on + strand, within Psyr_2997 at 3592.762 kb on - strand, within Psyr_2997 at 3592.800 kb on + strand, within Psyr_2997 at 3592.800 kb on + strand, within Psyr_2997 at 3592.801 kb on - strand, within Psyr_2997 at 3592.930 kb on - strand, within Psyr_2997 at 3593.019 kb on + strand, within Psyr_2997 at 3593.086 kb on + strand, within Psyr_2997 at 3593.265 kb on + strand, within Psyr_2997 at 3593.266 kb on - strand, within Psyr_2997 at 3593.275 kb on - strand, within Psyr_2997 at 3593.466 kb on + strand, within Psyr_2997 at 3593.499 kb on + strand, within Psyr_2997 at 3593.499 kb on + strand, within Psyr_2997 at 3593.499 kb on + strand, within Psyr_2997 at 3593.500 kb on - strand, within Psyr_2997 at 3593.500 kb on - strand, within Psyr_2997 at 3593.500 kb on - strand, within Psyr_2997 at 3593.500 kb on - strand, within Psyr_2997 at 3593.500 kb on - strand, within Psyr_2997 at 3593.500 kb on - strand, within Psyr_2997 at 3593.551 kb on + strand, within Psyr_2997 at 3593.973 kb on + strand, within Psyr_2997 at 3594.108 kb on - strand, within Psyr_2997 at 3594.342 kb on + strand, within Psyr_2997 at 3594.342 kb on + strand, within Psyr_2997 at 3594.343 kb on - strand, within Psyr_2997 at 3594.343 kb on - strand, within Psyr_2997 at 3594.343 kb on - strand, within Psyr_2997 at 3594.343 kb on - strand, within Psyr_2997 at 3594.576 kb on + strand at 3594.645 kb on + strand at 3594.645 kb on + strand at 3594.645 kb on + strand at 3594.645 kb on + strand at 3594.646 kb on - strand at 3594.646 kb on - strand at 3594.646 kb on - strand at 3594.778 kb on - strand at 3594.778 kb on - strand at 3594.778 kb on - strand at 3594.896 kb on + strand, within Psyr_2998 at 3594.897 kb on - strand, within Psyr_2998 at 3595.009 kb on + strand, within Psyr_2998 at 3595.263 kb on - strand, within Psyr_2998 at 3595.511 kb on + strand, within Psyr_2998 at 3595.512 kb on - strand, within Psyr_2998 at 3595.512 kb on - strand, within Psyr_2998 at 3595.512 kb on - strand, within Psyr_2998 at 3595.512 kb on - strand, within Psyr_2998 at 3595.512 kb on - strand, within Psyr_2998 at 3595.575 kb on - strand, within Psyr_2998
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Khayasin C 10 mM remove 3,591,643 + Psyr_2995 0.89 -0.8 3,591,729 - -1.0 3,591,752 + +0.6 3,591,752 + +1.2 3,591,753 - +0.5 3,591,887 + +0.0 3,591,930 + -1.3 3,591,939 + -1.2 3,591,939 + -0.7 3,591,939 + -0.0 3,591,939 + +1.1 3,591,939 + +1.5 3,591,939 + +0.5 3,591,939 + +0.3 3,591,939 + +0.1 3,591,940 - -0.5 3,591,940 - -1.4 3,591,940 - -1.9 3,591,940 - -0.5 3,591,943 + +0.9 3,591,943 + -1.2 3,591,943 + +0.4 3,591,943 + -0.9 3,591,944 - -0.9 3,591,944 - -0.7 3,591,944 - -1.3 3,591,944 - -0.6 3,591,944 - +1.2 3,591,944 - -1.0 3,591,946 + -1.8 3,591,946 + +1.5 3,591,947 - +0.4 3,591,947 - -1.9 3,591,947 - -0.4 3,591,947 - +0.5 3,592,142 - Psyr_2996 0.45 +0.4 3,592,393 - -0.0 3,592,393 - -0.4 3,592,429 + -0.5 3,592,539 - +1.6 3,592,539 - +0.4 3,592,553 + -2.2 3,592,553 + -1.7 3,592,554 - -0.0 3,592,685 - -0.2 3,592,761 + Psyr_2997 0.11 -0.2 3,592,762 - Psyr_2997 0.11 +0.5 3,592,800 + Psyr_2997 0.13 +1.5 3,592,800 + Psyr_2997 0.13 -0.9 3,592,801 - Psyr_2997 0.13 -0.2 3,592,930 - Psyr_2997 0.19 -0.1 3,593,019 + Psyr_2997 0.23 -0.4 3,593,086 + Psyr_2997 0.26 -0.4 3,593,265 + Psyr_2997 0.34 +1.6 3,593,266 - Psyr_2997 0.34 +0.6 3,593,275 - Psyr_2997 0.35 -0.2 3,593,466 + Psyr_2997 0.43 -0.4 3,593,499 + Psyr_2997 0.45 -1.2 3,593,499 + Psyr_2997 0.45 -0.8 3,593,499 + Psyr_2997 0.45 +0.6 3,593,500 - Psyr_2997 0.45 +1.2 3,593,500 - Psyr_2997 0.45 -1.8 3,593,500 - Psyr_2997 0.45 -0.5 3,593,500 - Psyr_2997 0.45 -1.1 3,593,500 - Psyr_2997 0.45 +2.5 3,593,500 - Psyr_2997 0.45 -0.2 3,593,551 + Psyr_2997 0.47 +1.4 3,593,973 + Psyr_2997 0.66 +0.2 3,594,108 - Psyr_2997 0.73 -1.4 3,594,342 + Psyr_2997 0.83 +2.2 3,594,342 + Psyr_2997 0.83 +0.6 3,594,343 - Psyr_2997 0.83 +1.1 3,594,343 - Psyr_2997 0.83 +0.5 3,594,343 - Psyr_2997 0.83 +1.0 3,594,343 - Psyr_2997 0.83 +0.2 3,594,576 + -0.3 3,594,645 + +1.0 3,594,645 + +0.1 3,594,645 + +0.0 3,594,645 + +3.0 3,594,646 - +0.4 3,594,646 - +1.8 3,594,646 - +0.0 3,594,778 - -0.9 3,594,778 - -1.0 3,594,778 - +2.0 3,594,896 + Psyr_2998 0.19 +0.2 3,594,897 - Psyr_2998 0.19 +0.3 3,595,009 + Psyr_2998 0.30 +1.2 3,595,263 - Psyr_2998 0.55 +2.2 3,595,511 + Psyr_2998 0.80 +0.9 3,595,512 - Psyr_2998 0.80 +0.6 3,595,512 - Psyr_2998 0.80 -0.8 3,595,512 - Psyr_2998 0.80 +0.4 3,595,512 - Psyr_2998 0.80 +0.2 3,595,512 - Psyr_2998 0.80 +1.1 3,595,575 - Psyr_2998 0.86 +0.3
Or see this region's nucleotide sequence