Experiment: KB with Khayasin C 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2453 and Psyr_2454 overlap by 33 nucleotides Psyr_2454 and Psyr_2455 are separated by 29 nucleotides Psyr_2455 and Psyr_2456 are separated by 100 nucleotides Psyr_2456 and Psyr_2457 are separated by 27 nucleotides
Psyr_2453: Psyr_2453 - NUDIX hydrolase, at 2,841,626 to 2,842,462
_2453
Psyr_2454: Psyr_2454 - Protein of unknown function DUF81, at 2,842,430 to 2,843,272
_2454
Psyr_2455: Psyr_2455 - Oxidoreductase, N-terminal:Oxidoreductase, C-terminal, at 2,843,302 to 2,844,450
_2455
Psyr_2456: Psyr_2456 - Short-chain dehydrogenase/reductase SDR, at 2,844,551 to 2,845,318
_2456
Psyr_2457: Psyr_2457 - Aminoglycoside phosphotransferase, at 2,845,346 to 2,846,413
_2457
Position (kb)
2843
2844
2845 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2842.416 kb on - strand at 2842.416 kb on - strand at 2842.461 kb on - strand at 2842.466 kb on + strand at 2842.548 kb on + strand, within Psyr_2454 at 2842.548 kb on + strand, within Psyr_2454 at 2842.549 kb on - strand, within Psyr_2454 at 2842.549 kb on - strand, within Psyr_2454 at 2842.549 kb on - strand, within Psyr_2454 at 2842.593 kb on + strand, within Psyr_2454 at 2842.593 kb on + strand, within Psyr_2454 at 2842.593 kb on + strand, within Psyr_2454 at 2842.593 kb on + strand, within Psyr_2454 at 2842.594 kb on - strand, within Psyr_2454 at 2842.594 kb on - strand, within Psyr_2454 at 2842.594 kb on - strand, within Psyr_2454 at 2842.594 kb on - strand, within Psyr_2454 at 2842.594 kb on - strand, within Psyr_2454 at 2842.594 kb on - strand, within Psyr_2454 at 2842.756 kb on - strand, within Psyr_2454 at 2842.851 kb on + strand, within Psyr_2454 at 2842.851 kb on + strand, within Psyr_2454 at 2842.852 kb on - strand, within Psyr_2454 at 2842.852 kb on - strand, within Psyr_2454 at 2842.852 kb on - strand, within Psyr_2454 at 2842.852 kb on - strand, within Psyr_2454 at 2842.912 kb on - strand, within Psyr_2454 at 2842.912 kb on - strand at 2843.001 kb on + strand, within Psyr_2454 at 2843.001 kb on + strand, within Psyr_2454 at 2843.136 kb on - strand, within Psyr_2454 at 2843.276 kb on - strand at 2843.284 kb on + strand at 2843.284 kb on + strand at 2843.284 kb on + strand at 2843.284 kb on + strand at 2843.285 kb on - strand at 2843.285 kb on - strand at 2843.417 kb on + strand, within Psyr_2455 at 2843.418 kb on - strand, within Psyr_2455 at 2843.440 kb on + strand, within Psyr_2455 at 2843.440 kb on + strand, within Psyr_2455 at 2843.441 kb on - strand, within Psyr_2455 at 2843.465 kb on + strand, within Psyr_2455 at 2843.565 kb on + strand, within Psyr_2455 at 2843.570 kb on + strand, within Psyr_2455 at 2843.655 kb on - strand, within Psyr_2455 at 2843.788 kb on + strand, within Psyr_2455 at 2844.032 kb on + strand, within Psyr_2455 at 2844.033 kb on - strand, within Psyr_2455 at 2844.033 kb on - strand, within Psyr_2455 at 2844.033 kb on - strand, within Psyr_2455 at 2844.033 kb on - strand, within Psyr_2455 at 2844.038 kb on + strand, within Psyr_2455 at 2844.128 kb on + strand, within Psyr_2455 at 2844.208 kb on + strand, within Psyr_2455 at 2844.208 kb on + strand, within Psyr_2455 at 2844.595 kb on + strand at 2844.596 kb on - strand at 2844.597 kb on + strand at 2844.597 kb on + strand at 2844.597 kb on + strand at 2844.597 kb on + strand at 2844.598 kb on - strand at 2844.769 kb on + strand, within Psyr_2456 at 2845.095 kb on - strand, within Psyr_2456 at 2845.095 kb on - strand, within Psyr_2456 at 2845.302 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Khayasin C 10 mM remove 2,842,416 - +0.8 2,842,416 - +2.0 2,842,461 - +0.1 2,842,466 + +0.5 2,842,548 + Psyr_2454 0.14 +0.7 2,842,548 + Psyr_2454 0.14 +0.0 2,842,549 - Psyr_2454 0.14 -0.1 2,842,549 - Psyr_2454 0.14 +0.2 2,842,549 - Psyr_2454 0.14 -0.5 2,842,593 + Psyr_2454 0.19 -1.0 2,842,593 + Psyr_2454 0.19 +0.1 2,842,593 + Psyr_2454 0.19 +1.2 2,842,593 + Psyr_2454 0.19 -0.1 2,842,594 - Psyr_2454 0.19 +0.4 2,842,594 - Psyr_2454 0.19 +0.1 2,842,594 - Psyr_2454 0.19 -0.3 2,842,594 - Psyr_2454 0.19 +1.6 2,842,594 - Psyr_2454 0.19 -0.1 2,842,594 - Psyr_2454 0.19 +0.0 2,842,756 - Psyr_2454 0.39 +2.4 2,842,851 + Psyr_2454 0.50 -0.5 2,842,851 + Psyr_2454 0.50 +1.0 2,842,852 - Psyr_2454 0.50 +1.7 2,842,852 - Psyr_2454 0.50 +1.0 2,842,852 - Psyr_2454 0.50 -0.2 2,842,852 - Psyr_2454 0.50 -1.6 2,842,912 - Psyr_2454 0.57 +0.3 2,842,912 - +0.4 2,843,001 + Psyr_2454 0.68 +0.5 2,843,001 + Psyr_2454 0.68 +0.4 2,843,136 - Psyr_2454 0.84 +2.9 2,843,276 - +1.8 2,843,284 + +1.1 2,843,284 + +0.0 2,843,284 + +0.1 2,843,284 + -0.1 2,843,285 - +0.0 2,843,285 - -0.9 2,843,417 + Psyr_2455 0.10 -1.1 2,843,418 - Psyr_2455 0.10 -0.4 2,843,440 + Psyr_2455 0.12 -0.4 2,843,440 + Psyr_2455 0.12 +0.5 2,843,441 - Psyr_2455 0.12 -0.2 2,843,465 + Psyr_2455 0.14 +1.4 2,843,565 + Psyr_2455 0.23 +0.4 2,843,570 + Psyr_2455 0.23 -0.8 2,843,655 - Psyr_2455 0.31 -0.8 2,843,788 + Psyr_2455 0.42 +1.2 2,844,032 + Psyr_2455 0.64 +1.2 2,844,033 - Psyr_2455 0.64 -0.5 2,844,033 - Psyr_2455 0.64 -0.1 2,844,033 - Psyr_2455 0.64 -0.5 2,844,033 - Psyr_2455 0.64 -2.0 2,844,038 + Psyr_2455 0.64 -0.9 2,844,128 + Psyr_2455 0.72 +0.5 2,844,208 + Psyr_2455 0.79 -0.6 2,844,208 + Psyr_2455 0.79 -0.8 2,844,595 + -1.1 2,844,596 - +0.7 2,844,597 + +0.1 2,844,597 + +1.6 2,844,597 + +0.6 2,844,597 + -0.9 2,844,598 - +0.1 2,844,769 + Psyr_2456 0.28 -0.9 2,845,095 - Psyr_2456 0.71 +0.6 2,845,095 - Psyr_2456 0.71 -1.0 2,845,302 - +1.2
Or see this region's nucleotide sequence