Experiment: KB with Khayasin C 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2031 and Psyr_2032 overlap by 4 nucleotides Psyr_2032 and Psyr_2033 are separated by 379 nucleotides
Psyr_2031: Psyr_2031 - ATP-binding region, ATPase-like:Histidine kinase, HAMP region:Histidine kinase A, N-terminal, at 2,355,285 to 2,356,319
_2031
Psyr_2032: Psyr_2032 - two component transcriptional regulator, winged helix family, at 2,356,316 to 2,357,095
_2032
Psyr_2033: Psyr_2033 - conserved hypothetical protein, at 2,357,475 to 2,359,553
_2033
Position (kb)
2356
2357
2358 Strain fitness (log2 ratio)
-1
0
1
2 at 2356.113 kb on + strand, within Psyr_2031 at 2356.113 kb on + strand, within Psyr_2031 at 2356.113 kb on + strand, within Psyr_2031 at 2356.113 kb on + strand, within Psyr_2031 at 2356.113 kb on + strand, within Psyr_2031 at 2356.114 kb on - strand, within Psyr_2031 at 2356.114 kb on - strand, within Psyr_2031 at 2356.344 kb on + strand at 2356.719 kb on + strand, within Psyr_2032 at 2356.719 kb on + strand, within Psyr_2032 at 2356.719 kb on + strand, within Psyr_2032 at 2356.719 kb on + strand, within Psyr_2032 at 2356.719 kb on + strand, within Psyr_2032 at 2356.719 kb on + strand, within Psyr_2032 at 2356.719 kb on + strand, within Psyr_2032 at 2356.719 kb on + strand, within Psyr_2032 at 2356.720 kb on - strand, within Psyr_2032 at 2356.720 kb on - strand, within Psyr_2032 at 2356.720 kb on - strand, within Psyr_2032 at 2356.720 kb on - strand, within Psyr_2032 at 2356.804 kb on + strand, within Psyr_2032 at 2356.804 kb on + strand, within Psyr_2032 at 2356.804 kb on + strand, within Psyr_2032 at 2356.805 kb on - strand, within Psyr_2032 at 2356.937 kb on - strand, within Psyr_2032 at 2357.065 kb on + strand at 2357.066 kb on - strand at 2357.135 kb on + strand at 2357.135 kb on + strand at 2357.136 kb on - strand at 2357.136 kb on - strand at 2357.192 kb on + strand at 2357.192 kb on + strand at 2357.193 kb on - strand at 2357.198 kb on + strand at 2357.199 kb on - strand at 2357.203 kb on - strand at 2357.203 kb on - strand at 2357.203 kb on - strand at 2357.203 kb on - strand at 2357.203 kb on - strand at 2357.253 kb on + strand at 2357.435 kb on + strand at 2357.436 kb on - strand at 2357.436 kb on - strand at 2357.436 kb on - strand at 2357.439 kb on + strand at 2357.439 kb on + strand at 2357.439 kb on + strand at 2357.883 kb on + strand, within Psyr_2033 at 2357.883 kb on + strand, within Psyr_2033 at 2357.883 kb on + strand, within Psyr_2033 at 2357.884 kb on - strand, within Psyr_2033 at 2358.037 kb on + strand, within Psyr_2033 at 2358.037 kb on + strand, within Psyr_2033 at 2358.037 kb on + strand, within Psyr_2033 at 2358.037 kb on + strand, within Psyr_2033 at 2358.037 kb on + strand, within Psyr_2033 at 2358.038 kb on - strand, within Psyr_2033 at 2358.038 kb on - strand, within Psyr_2033 at 2358.038 kb on - strand, within Psyr_2033 at 2358.038 kb on - strand, within Psyr_2033 at 2358.038 kb on - strand, within Psyr_2033 at 2358.038 kb on - strand, within Psyr_2033 at 2358.072 kb on + strand, within Psyr_2033 at 2358.073 kb on - strand, within Psyr_2033 at 2358.073 kb on - strand, within Psyr_2033
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Khayasin C 10 mM remove 2,356,113 + Psyr_2031 0.80 +1.0 2,356,113 + Psyr_2031 0.80 -0.2 2,356,113 + Psyr_2031 0.80 -0.2 2,356,113 + Psyr_2031 0.80 +0.3 2,356,113 + Psyr_2031 0.80 -0.9 2,356,114 - Psyr_2031 0.80 +0.3 2,356,114 - Psyr_2031 0.80 +1.1 2,356,344 + -0.1 2,356,719 + Psyr_2032 0.52 +0.4 2,356,719 + Psyr_2032 0.52 +1.0 2,356,719 + Psyr_2032 0.52 -0.6 2,356,719 + Psyr_2032 0.52 +1.4 2,356,719 + Psyr_2032 0.52 +0.4 2,356,719 + Psyr_2032 0.52 +1.3 2,356,719 + Psyr_2032 0.52 -1.1 2,356,719 + Psyr_2032 0.52 +0.9 2,356,720 - Psyr_2032 0.52 +0.8 2,356,720 - Psyr_2032 0.52 +0.9 2,356,720 - Psyr_2032 0.52 +0.4 2,356,720 - Psyr_2032 0.52 -0.4 2,356,804 + Psyr_2032 0.63 -0.2 2,356,804 + Psyr_2032 0.63 +0.4 2,356,804 + Psyr_2032 0.63 +2.3 2,356,805 - Psyr_2032 0.63 -0.9 2,356,937 - Psyr_2032 0.80 +1.3 2,357,065 + +0.5 2,357,066 - -0.3 2,357,135 + +0.5 2,357,135 + +0.8 2,357,136 - -0.2 2,357,136 - +0.1 2,357,192 + +0.4 2,357,192 + -1.0 2,357,193 - +1.1 2,357,198 + -0.3 2,357,199 - +0.2 2,357,203 - -0.4 2,357,203 - +0.6 2,357,203 - -0.2 2,357,203 - +0.2 2,357,203 - -0.2 2,357,253 + +0.1 2,357,435 + -0.3 2,357,436 - +0.3 2,357,436 - +0.6 2,357,436 - -0.7 2,357,439 + +1.3 2,357,439 + +1.7 2,357,439 + +0.2 2,357,883 + Psyr_2033 0.20 -1.2 2,357,883 + Psyr_2033 0.20 +0.3 2,357,883 + Psyr_2033 0.20 +2.3 2,357,884 - Psyr_2033 0.20 +0.1 2,358,037 + Psyr_2033 0.27 -1.2 2,358,037 + Psyr_2033 0.27 -0.6 2,358,037 + Psyr_2033 0.27 +0.3 2,358,037 + Psyr_2033 0.27 -0.0 2,358,037 + Psyr_2033 0.27 -0.1 2,358,038 - Psyr_2033 0.27 +1.5 2,358,038 - Psyr_2033 0.27 +0.3 2,358,038 - Psyr_2033 0.27 +1.0 2,358,038 - Psyr_2033 0.27 -0.6 2,358,038 - Psyr_2033 0.27 +0.2 2,358,038 - Psyr_2033 0.27 -1.0 2,358,072 + Psyr_2033 0.29 -0.3 2,358,073 - Psyr_2033 0.29 -0.6 2,358,073 - Psyr_2033 0.29 -0.6
Or see this region's nucleotide sequence