Experiment: KB with Khayasin C 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1272 and Psyr_1273 are separated by 150 nucleotides Psyr_1273 and Psyr_1274 are separated by 69 nucleotides Psyr_1274 and Psyr_1275 overlap by 1 nucleotides
Psyr_1272: Psyr_1272 - NUDIX hydrolase, at 1,438,297 to 1,438,848
_1272
Psyr_1273: Psyr_1273 - NUDIX hydrolase, at 1,438,999 to 1,439,595
_1273
Psyr_1274: Psyr_1274 - transferase hexapeptide repeat protein, at 1,439,665 to 1,440,189
_1274
Psyr_1275: Psyr_1275 - Protein of unknown function DUF1289, at 1,440,189 to 1,440,407
_1275
Position (kb)
1439
1440
1441 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1438.749 kb on - strand, within Psyr_1272 at 1438.778 kb on + strand, within Psyr_1272 at 1438.778 kb on + strand, within Psyr_1272 at 1439.012 kb on + strand at 1439.012 kb on + strand at 1439.012 kb on + strand at 1439.012 kb on + strand at 1439.012 kb on + strand at 1439.012 kb on + strand at 1439.012 kb on + strand at 1439.012 kb on + strand at 1439.013 kb on - strand at 1439.013 kb on - strand at 1439.013 kb on - strand at 1439.013 kb on - strand at 1439.013 kb on - strand at 1439.013 kb on - strand at 1439.025 kb on - strand at 1439.323 kb on + strand, within Psyr_1273 at 1439.323 kb on + strand, within Psyr_1273 at 1439.323 kb on + strand, within Psyr_1273 at 1439.323 kb on + strand, within Psyr_1273 at 1439.324 kb on - strand, within Psyr_1273 at 1439.464 kb on + strand, within Psyr_1273 at 1439.464 kb on + strand, within Psyr_1273 at 1439.465 kb on - strand, within Psyr_1273 at 1439.477 kb on - strand, within Psyr_1273 at 1439.492 kb on - strand, within Psyr_1273 at 1439.492 kb on - strand, within Psyr_1273 at 1439.531 kb on + strand, within Psyr_1273 at 1439.545 kb on + strand at 1439.546 kb on - strand at 1439.546 kb on - strand at 1439.625 kb on + strand at 1439.625 kb on + strand at 1439.625 kb on + strand at 1439.625 kb on + strand at 1439.626 kb on - strand at 1439.626 kb on - strand at 1439.626 kb on - strand at 1439.626 kb on - strand at 1439.626 kb on - strand at 1439.626 kb on - strand at 1439.626 kb on - strand at 1439.626 kb on - strand at 1439.626 kb on - strand at 1439.626 kb on - strand at 1439.626 kb on - strand at 1439.626 kb on - strand at 1439.626 kb on - strand at 1439.626 kb on - strand at 1439.626 kb on - strand at 1439.643 kb on - strand at 1439.942 kb on - strand, within Psyr_1274 at 1439.995 kb on + strand, within Psyr_1274 at 1439.996 kb on - strand, within Psyr_1274 at 1439.996 kb on - strand, within Psyr_1274 at 1439.996 kb on - strand, within Psyr_1274 at 1439.996 kb on - strand, within Psyr_1274 at 1440.137 kb on - strand at 1440.137 kb on - strand at 1440.137 kb on - strand at 1440.140 kb on + strand at 1440.140 kb on + strand at 1440.140 kb on + strand at 1440.141 kb on - strand at 1440.141 kb on - strand at 1440.141 kb on - strand at 1440.141 kb on - strand at 1440.141 kb on - strand at 1440.141 kb on - strand at 1440.141 kb on - strand at 1440.141 kb on - strand at 1440.141 kb on - strand at 1440.239 kb on + strand, within Psyr_1275 at 1440.239 kb on + strand, within Psyr_1275 at 1440.239 kb on + strand, within Psyr_1275 at 1440.240 kb on - strand, within Psyr_1275 at 1440.240 kb on - strand, within Psyr_1275 at 1440.240 kb on - strand, within Psyr_1275 at 1440.240 kb on - strand, within Psyr_1275 at 1440.263 kb on + strand, within Psyr_1275 at 1440.264 kb on - strand, within Psyr_1275 at 1440.424 kb on + strand at 1440.424 kb on + strand at 1440.424 kb on + strand at 1440.425 kb on - strand at 1440.425 kb on - strand at 1440.425 kb on - strand at 1440.425 kb on - strand at 1440.841 kb on + strand at 1440.864 kb on + strand at 1441.170 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Khayasin C 10 mM remove 1,438,749 - Psyr_1272 0.82 -0.8 1,438,778 + Psyr_1272 0.87 +0.2 1,438,778 + Psyr_1272 0.87 +1.9 1,439,012 + -1.5 1,439,012 + +0.0 1,439,012 + -0.2 1,439,012 + +0.5 1,439,012 + +0.5 1,439,012 + -1.0 1,439,012 + -1.5 1,439,012 + +0.1 1,439,013 - -0.2 1,439,013 - -1.6 1,439,013 - +0.6 1,439,013 - -0.1 1,439,013 - -1.0 1,439,013 - +0.5 1,439,025 - +0.0 1,439,323 + Psyr_1273 0.54 +0.2 1,439,323 + Psyr_1273 0.54 +0.8 1,439,323 + Psyr_1273 0.54 -1.1 1,439,323 + Psyr_1273 0.54 -0.2 1,439,324 - Psyr_1273 0.54 +0.1 1,439,464 + Psyr_1273 0.78 -0.2 1,439,464 + Psyr_1273 0.78 -0.2 1,439,465 - Psyr_1273 0.78 -0.0 1,439,477 - Psyr_1273 0.80 +0.1 1,439,492 - Psyr_1273 0.83 +0.3 1,439,492 - Psyr_1273 0.83 -0.2 1,439,531 + Psyr_1273 0.89 +0.4 1,439,545 + +0.3 1,439,546 - -0.4 1,439,546 - -0.4 1,439,625 + -1.5 1,439,625 + -0.2 1,439,625 + -0.6 1,439,625 + +1.0 1,439,626 - +0.4 1,439,626 - +0.6 1,439,626 - +0.2 1,439,626 - -0.4 1,439,626 - +0.2 1,439,626 - +0.3 1,439,626 - +1.8 1,439,626 - +0.5 1,439,626 - -1.5 1,439,626 - +0.8 1,439,626 - -0.5 1,439,626 - -0.3 1,439,626 - +0.0 1,439,626 - +0.3 1,439,626 - +1.3 1,439,643 - +0.5 1,439,942 - Psyr_1274 0.53 +0.7 1,439,995 + Psyr_1274 0.63 +1.8 1,439,996 - Psyr_1274 0.63 +0.0 1,439,996 - Psyr_1274 0.63 -1.2 1,439,996 - Psyr_1274 0.63 +1.5 1,439,996 - Psyr_1274 0.63 -0.6 1,440,137 - -0.8 1,440,137 - -1.0 1,440,137 - +0.8 1,440,140 + +1.0 1,440,140 + +0.3 1,440,140 + +0.5 1,440,141 - -0.9 1,440,141 - +0.8 1,440,141 - -0.1 1,440,141 - +0.2 1,440,141 - -0.3 1,440,141 - -0.6 1,440,141 - -0.1 1,440,141 - +0.3 1,440,141 - +0.5 1,440,239 + Psyr_1275 0.23 +0.5 1,440,239 + Psyr_1275 0.23 +0.8 1,440,239 + Psyr_1275 0.23 +0.6 1,440,240 - Psyr_1275 0.23 -0.7 1,440,240 - Psyr_1275 0.23 +0.8 1,440,240 - Psyr_1275 0.23 -0.2 1,440,240 - Psyr_1275 0.23 -1.7 1,440,263 + Psyr_1275 0.34 -0.0 1,440,264 - Psyr_1275 0.34 +0.2 1,440,424 + +1.3 1,440,424 + -1.0 1,440,424 + -1.8 1,440,425 - -0.3 1,440,425 - +0.6 1,440,425 - -1.5 1,440,425 - +1.8 1,440,841 + +0.5 1,440,864 + +0.1 1,441,170 + +0.8
Or see this region's nucleotide sequence