Experiment: KB with Khayasin C 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0306 and Psyr_0307 are separated by 586 nucleotides Psyr_0307 and Psyr_0308 are separated by 288 nucleotides
Psyr_0306: Psyr_0306 - TonB, C-terminal, at 326,643 to 327,308
_0306
Psyr_0307: Psyr_0307 - hypothetical protein, at 327,895 to 328,398
_0307
Psyr_0308: Psyr_0308 - hypothetical protein, at 328,687 to 329,424
_0308
Position (kb)
327
328
329 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 326.895 kb on - strand, within Psyr_0306 at 326.895 kb on - strand, within Psyr_0306 at 327.080 kb on + strand, within Psyr_0306 at 327.099 kb on + strand, within Psyr_0306 at 327.100 kb on - strand, within Psyr_0306 at 327.100 kb on - strand at 327.243 kb on + strand at 327.243 kb on + strand at 327.244 kb on - strand at 327.244 kb on - strand at 327.244 kb on - strand at 327.244 kb on - strand at 327.244 kb on - strand at 327.325 kb on + strand at 327.325 kb on + strand at 327.325 kb on + strand at 327.325 kb on + strand at 327.325 kb on + strand at 327.325 kb on + strand at 327.326 kb on - strand at 327.326 kb on - strand at 327.326 kb on - strand at 327.326 kb on - strand at 327.326 kb on - strand at 327.326 kb on - strand at 327.326 kb on - strand at 327.354 kb on + strand at 327.355 kb on - strand at 327.537 kb on + strand at 327.537 kb on + strand at 327.538 kb on - strand at 327.585 kb on + strand at 327.646 kb on + strand at 327.669 kb on + strand at 327.670 kb on - strand at 327.670 kb on - strand at 327.670 kb on - strand at 327.779 kb on + strand at 327.779 kb on + strand at 327.806 kb on + strand at 327.806 kb on + strand at 327.807 kb on - strand at 327.813 kb on + strand at 327.813 kb on + strand at 327.814 kb on - strand at 327.837 kb on - strand at 327.837 kb on - strand at 327.842 kb on - strand at 327.931 kb on - strand at 327.996 kb on - strand, within Psyr_0307 at 328.012 kb on - strand, within Psyr_0307 at 328.029 kb on - strand, within Psyr_0307 at 328.044 kb on + strand, within Psyr_0307 at 328.047 kb on + strand, within Psyr_0307 at 328.047 kb on + strand, within Psyr_0307 at 328.117 kb on + strand, within Psyr_0307 at 328.121 kb on + strand, within Psyr_0307 at 328.178 kb on - strand, within Psyr_0307 at 328.214 kb on + strand, within Psyr_0307 at 328.214 kb on + strand at 328.214 kb on + strand, within Psyr_0307 at 328.257 kb on + strand, within Psyr_0307 at 328.257 kb on + strand, within Psyr_0307 at 328.257 kb on + strand, within Psyr_0307 at 328.258 kb on - strand, within Psyr_0307 at 328.258 kb on - strand, within Psyr_0307 at 328.294 kb on + strand, within Psyr_0307 at 328.295 kb on - strand, within Psyr_0307 at 328.321 kb on - strand, within Psyr_0307 at 328.362 kb on + strand at 328.363 kb on - strand at 328.363 kb on - strand at 328.392 kb on + strand at 328.411 kb on - strand at 328.424 kb on + strand at 328.439 kb on + strand at 328.439 kb on + strand at 328.440 kb on - strand at 328.440 kb on - strand at 328.440 kb on - strand at 328.443 kb on + strand at 328.443 kb on + strand at 328.444 kb on - strand at 328.444 kb on - strand at 328.467 kb on - strand at 328.472 kb on - strand at 328.510 kb on - strand at 328.531 kb on - strand at 328.575 kb on - strand at 328.575 kb on - strand at 328.575 kb on - strand at 328.575 kb on - strand at 328.583 kb on + strand at 328.583 kb on + strand at 328.584 kb on - strand at 328.613 kb on + strand at 328.614 kb on - strand at 328.704 kb on + strand at 328.704 kb on + strand at 328.705 kb on - strand at 328.966 kb on + strand, within Psyr_0308 at 328.966 kb on + strand, within Psyr_0308 at 328.966 kb on + strand, within Psyr_0308 at 328.967 kb on - strand, within Psyr_0308 at 328.967 kb on - strand, within Psyr_0308 at 328.967 kb on - strand, within Psyr_0308 at 328.967 kb on - strand, within Psyr_0308 at 328.967 kb on - strand, within Psyr_0308 at 328.967 kb on - strand, within Psyr_0308 at 328.967 kb on - strand, within Psyr_0308 at 329.098 kb on + strand, within Psyr_0308 at 329.098 kb on + strand, within Psyr_0308 at 329.098 kb on + strand, within Psyr_0308 at 329.098 kb on + strand, within Psyr_0308 at 329.098 kb on + strand, within Psyr_0308 at 329.099 kb on - strand, within Psyr_0308 at 329.101 kb on + strand, within Psyr_0308 at 329.101 kb on + strand, within Psyr_0308 at 329.113 kb on + strand, within Psyr_0308 at 329.113 kb on + strand, within Psyr_0308 at 329.113 kb on + strand, within Psyr_0308 at 329.114 kb on - strand, within Psyr_0308 at 329.114 kb on - strand, within Psyr_0308 at 329.114 kb on - strand, within Psyr_0308 at 329.114 kb on - strand, within Psyr_0308 at 329.114 kb on - strand, within Psyr_0308 at 329.168 kb on + strand, within Psyr_0308 at 329.168 kb on + strand, within Psyr_0308 at 329.168 kb on + strand, within Psyr_0308 at 329.169 kb on - strand, within Psyr_0308 at 329.169 kb on - strand, within Psyr_0308 at 329.221 kb on + strand, within Psyr_0308 at 329.221 kb on + strand, within Psyr_0308 at 329.221 kb on + strand, within Psyr_0308 at 329.221 kb on + strand, within Psyr_0308 at 329.222 kb on - strand, within Psyr_0308 at 329.238 kb on + strand, within Psyr_0308 at 329.254 kb on - strand, within Psyr_0308 at 329.254 kb on - strand, within Psyr_0308 at 329.256 kb on - strand, within Psyr_0308 at 329.308 kb on + strand, within Psyr_0308 at 329.308 kb on + strand, within Psyr_0308 at 329.308 kb on + strand, within Psyr_0308 at 329.308 kb on + strand, within Psyr_0308 at 329.309 kb on - strand, within Psyr_0308 at 329.309 kb on - strand, within Psyr_0308 at 329.330 kb on - strand, within Psyr_0308 at 329.330 kb on - strand, within Psyr_0308 at 329.333 kb on + strand, within Psyr_0308 at 329.334 kb on - strand, within Psyr_0308 at 329.334 kb on - strand, within Psyr_0308 at 329.334 kb on - strand, within Psyr_0308 at 329.334 kb on - strand, within Psyr_0308 at 329.334 kb on - strand, within Psyr_0308 at 329.361 kb on - strand at 329.381 kb on - strand at 329.381 kb on - strand at 329.381 kb on - strand at 329.392 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Khayasin C 10 mM remove 326,895 - Psyr_0306 0.38 +1.2 326,895 - Psyr_0306 0.38 -1.1 327,080 + Psyr_0306 0.66 +0.1 327,099 + Psyr_0306 0.68 +0.2 327,100 - Psyr_0306 0.69 +0.4 327,100 - -0.7 327,243 + +0.5 327,243 + -0.5 327,244 - -0.4 327,244 - +0.0 327,244 - +0.2 327,244 - -0.8 327,244 - +0.5 327,325 + +0.9 327,325 + +0.6 327,325 + -0.2 327,325 + +0.5 327,325 + +0.7 327,325 + -0.1 327,326 - -0.3 327,326 - -0.2 327,326 - +0.3 327,326 - +0.2 327,326 - +1.0 327,326 - -2.5 327,326 - -0.4 327,354 + +1.1 327,355 - -0.3 327,537 + +1.6 327,537 + -0.1 327,538 - -0.0 327,585 + -0.2 327,646 + -1.2 327,669 + +2.3 327,670 - -0.3 327,670 - +0.9 327,670 - -0.1 327,779 + -1.2 327,779 + -1.4 327,806 + +0.6 327,806 + -0.9 327,807 - -0.2 327,813 + -0.3 327,813 + +0.4 327,814 - +1.2 327,837 - -0.3 327,837 - -0.6 327,842 - +0.6 327,931 - +1.3 327,996 - Psyr_0307 0.20 +0.3 328,012 - Psyr_0307 0.23 -0.5 328,029 - Psyr_0307 0.27 -1.2 328,044 + Psyr_0307 0.30 -0.4 328,047 + Psyr_0307 0.30 -0.8 328,047 + Psyr_0307 0.30 -0.0 328,117 + Psyr_0307 0.44 -1.5 328,121 + Psyr_0307 0.45 -1.3 328,178 - Psyr_0307 0.56 +0.2 328,214 + Psyr_0307 0.63 -1.5 328,214 + +0.5 328,214 + Psyr_0307 0.63 -1.5 328,257 + Psyr_0307 0.72 -0.3 328,257 + Psyr_0307 0.72 -1.4 328,257 + Psyr_0307 0.72 -0.7 328,258 - Psyr_0307 0.72 +1.5 328,258 - Psyr_0307 0.72 +0.5 328,294 + Psyr_0307 0.79 -0.1 328,295 - Psyr_0307 0.79 -0.3 328,321 - Psyr_0307 0.85 +1.1 328,362 + +0.4 328,363 - +0.2 328,363 - -0.3 328,392 + -0.7 328,411 - -0.1 328,424 + -0.7 328,439 + +1.5 328,439 + +0.8 328,440 - +1.2 328,440 - +2.5 328,440 - +0.0 328,443 + -0.0 328,443 + +0.5 328,444 - +0.3 328,444 - -0.6 328,467 - +0.5 328,472 - -0.2 328,510 - +0.7 328,531 - +0.4 328,575 - +0.5 328,575 - -1.1 328,575 - +1.3 328,575 - -0.6 328,583 + -0.1 328,583 + +0.4 328,584 - -0.3 328,613 + +0.1 328,614 - +0.2 328,704 + -0.0 328,704 + +0.5 328,705 - +0.8 328,966 + Psyr_0308 0.38 +1.1 328,966 + Psyr_0308 0.38 +0.4 328,966 + Psyr_0308 0.38 -0.1 328,967 - Psyr_0308 0.38 -0.5 328,967 - Psyr_0308 0.38 +0.8 328,967 - Psyr_0308 0.38 +0.6 328,967 - Psyr_0308 0.38 -0.3 328,967 - Psyr_0308 0.38 -1.5 328,967 - Psyr_0308 0.38 -1.1 328,967 - Psyr_0308 0.38 +0.4 329,098 + Psyr_0308 0.56 +0.4 329,098 + Psyr_0308 0.56 -0.5 329,098 + Psyr_0308 0.56 +0.8 329,098 + Psyr_0308 0.56 +0.4 329,098 + Psyr_0308 0.56 -1.0 329,099 - Psyr_0308 0.56 +0.4 329,101 + Psyr_0308 0.56 -0.2 329,101 + Psyr_0308 0.56 -0.0 329,113 + Psyr_0308 0.58 +0.8 329,113 + Psyr_0308 0.58 -0.4 329,113 + Psyr_0308 0.58 +1.5 329,114 - Psyr_0308 0.58 +0.0 329,114 - Psyr_0308 0.58 -0.2 329,114 - Psyr_0308 0.58 +0.0 329,114 - Psyr_0308 0.58 +0.7 329,114 - Psyr_0308 0.58 +0.2 329,168 + Psyr_0308 0.65 +0.2 329,168 + Psyr_0308 0.65 +0.1 329,168 + Psyr_0308 0.65 -0.5 329,169 - Psyr_0308 0.65 -0.7 329,169 - Psyr_0308 0.65 -0.0 329,221 + Psyr_0308 0.72 +0.6 329,221 + Psyr_0308 0.72 +0.9 329,221 + Psyr_0308 0.72 -1.8 329,221 + Psyr_0308 0.72 -0.2 329,222 - Psyr_0308 0.72 +0.1 329,238 + Psyr_0308 0.75 -0.5 329,254 - Psyr_0308 0.77 -0.2 329,254 - Psyr_0308 0.77 -0.3 329,256 - Psyr_0308 0.77 +1.5 329,308 + Psyr_0308 0.84 -0.4 329,308 + Psyr_0308 0.84 -0.4 329,308 + Psyr_0308 0.84 -0.6 329,308 + Psyr_0308 0.84 -1.3 329,309 - Psyr_0308 0.84 +0.1 329,309 - Psyr_0308 0.84 +1.0 329,330 - Psyr_0308 0.87 +0.3 329,330 - Psyr_0308 0.87 -2.3 329,333 + Psyr_0308 0.88 +0.9 329,334 - Psyr_0308 0.88 -1.0 329,334 - Psyr_0308 0.88 -0.5 329,334 - Psyr_0308 0.88 +0.2 329,334 - Psyr_0308 0.88 -0.7 329,334 - Psyr_0308 0.88 +0.0 329,361 - -0.3 329,381 - -0.1 329,381 - +1.6 329,381 - +0.3 329,392 + -0.5
Or see this region's nucleotide sequence