Experiment: KB with Khayasin C 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0147 and Psyr_0148 are separated by 49 nucleotides Psyr_0148 and Psyr_0149 are separated by 77 nucleotides Psyr_0149 and Psyr_0150 are separated by 114 nucleotides
Psyr_0147: Psyr_0147 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding protein, at 159,893 to 160,822
_0147
Psyr_0148: Psyr_0148 - hypothetical protein, at 160,872 to 161,111
_0148
Psyr_0149: Psyr_0149 - succinate semialdehyde dehydrogenase, at 161,189 to 162,646
_0149
Psyr_0150: Psyr_0150 - Acetylornithine deacetylase (ArgE), at 162,761 to 163,918
_0150
Position (kb)
161
162
163 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 160.333 kb on + strand, within Psyr_0147 at 160.333 kb on + strand, within Psyr_0147 at 160.334 kb on - strand, within Psyr_0147 at 160.334 kb on - strand, within Psyr_0147 at 160.415 kb on + strand, within Psyr_0147 at 160.416 kb on - strand, within Psyr_0147 at 160.416 kb on - strand, within Psyr_0147 at 160.416 kb on - strand, within Psyr_0147 at 160.556 kb on - strand, within Psyr_0147 at 160.556 kb on - strand, within Psyr_0147 at 160.817 kb on + strand at 160.820 kb on + strand at 160.821 kb on - strand at 160.821 kb on - strand at 160.821 kb on - strand at 160.821 kb on - strand at 160.821 kb on - strand at 160.857 kb on + strand at 160.857 kb on + strand at 160.857 kb on + strand at 160.857 kb on + strand at 160.858 kb on - strand at 161.013 kb on + strand, within Psyr_0148 at 161.013 kb on + strand, within Psyr_0148 at 161.013 kb on + strand, within Psyr_0148 at 161.013 kb on + strand, within Psyr_0148 at 161.013 kb on + strand, within Psyr_0148 at 161.014 kb on - strand, within Psyr_0148 at 161.067 kb on + strand, within Psyr_0148 at 161.067 kb on + strand, within Psyr_0148 at 161.128 kb on + strand at 161.190 kb on + strand at 161.190 kb on + strand at 161.191 kb on - strand at 161.227 kb on - strand at 161.227 kb on - strand at 161.388 kb on + strand, within Psyr_0149 at 161.388 kb on + strand, within Psyr_0149 at 161.389 kb on - strand, within Psyr_0149 at 161.389 kb on - strand, within Psyr_0149 at 161.389 kb on - strand, within Psyr_0149 at 161.389 kb on - strand, within Psyr_0149 at 161.392 kb on - strand, within Psyr_0149 at 161.392 kb on - strand, within Psyr_0149 at 161.526 kb on - strand, within Psyr_0149 at 161.733 kb on + strand, within Psyr_0149 at 161.734 kb on - strand, within Psyr_0149 at 161.734 kb on - strand, within Psyr_0149 at 162.037 kb on + strand, within Psyr_0149 at 162.258 kb on + strand, within Psyr_0149 at 162.259 kb on - strand, within Psyr_0149 at 162.597 kb on + strand at 162.597 kb on + strand at 162.598 kb on - strand at 162.598 kb on - strand at 162.608 kb on + strand at 162.680 kb on - strand at 162.763 kb on - strand at 162.763 kb on - strand at 162.927 kb on + strand, within Psyr_0150 at 162.928 kb on - strand, within Psyr_0150 at 162.928 kb on - strand, within Psyr_0150 at 163.076 kb on + strand, within Psyr_0150 at 163.076 kb on + strand, within Psyr_0150 at 163.077 kb on - strand, within Psyr_0150 at 163.217 kb on + strand, within Psyr_0150 at 163.218 kb on - strand, within Psyr_0150 at 163.218 kb on - strand, within Psyr_0150 at 163.218 kb on - strand, within Psyr_0150 at 163.218 kb on - strand, within Psyr_0150 at 163.227 kb on + strand, within Psyr_0150 at 163.227 kb on + strand, within Psyr_0150 at 163.227 kb on + strand, within Psyr_0150 at 163.457 kb on + strand, within Psyr_0150 at 163.458 kb on - strand, within Psyr_0150 at 163.458 kb on - strand, within Psyr_0150 at 163.629 kb on + strand, within Psyr_0150 at 163.629 kb on + strand, within Psyr_0150 at 163.630 kb on - strand, within Psyr_0150 at 163.630 kb on - strand, within Psyr_0150 at 163.630 kb on - strand, within Psyr_0150 at 163.630 kb on - strand, within Psyr_0150
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Khayasin C 10 mM remove 160,333 + Psyr_0147 0.47 +0.2 160,333 + Psyr_0147 0.47 -1.5 160,334 - Psyr_0147 0.47 +1.1 160,334 - Psyr_0147 0.47 -0.5 160,415 + Psyr_0147 0.56 -0.6 160,416 - Psyr_0147 0.56 -0.1 160,416 - Psyr_0147 0.56 -1.1 160,416 - Psyr_0147 0.56 -0.4 160,556 - Psyr_0147 0.71 -1.8 160,556 - Psyr_0147 0.71 -0.2 160,817 + -0.8 160,820 + +0.2 160,821 - -1.3 160,821 - +1.3 160,821 - +0.5 160,821 - +0.3 160,821 - -1.3 160,857 + -1.4 160,857 + -0.6 160,857 + -0.1 160,857 + -1.6 160,858 - +0.5 161,013 + Psyr_0148 0.59 +0.6 161,013 + Psyr_0148 0.59 -0.6 161,013 + Psyr_0148 0.59 -1.1 161,013 + Psyr_0148 0.59 -1.1 161,013 + Psyr_0148 0.59 +1.4 161,014 - Psyr_0148 0.59 +0.7 161,067 + Psyr_0148 0.81 -0.1 161,067 + Psyr_0148 0.81 +0.5 161,128 + -0.6 161,190 + -0.2 161,190 + -1.7 161,191 - +0.7 161,227 - -0.4 161,227 - -0.4 161,388 + Psyr_0149 0.14 +0.5 161,388 + Psyr_0149 0.14 +2.5 161,389 - Psyr_0149 0.14 +0.5 161,389 - Psyr_0149 0.14 +0.5 161,389 - Psyr_0149 0.14 +0.2 161,389 - Psyr_0149 0.14 +0.8 161,392 - Psyr_0149 0.14 +0.7 161,392 - Psyr_0149 0.14 +1.4 161,526 - Psyr_0149 0.23 +0.5 161,733 + Psyr_0149 0.37 -2.0 161,734 - Psyr_0149 0.37 -1.0 161,734 - Psyr_0149 0.37 +0.3 162,037 + Psyr_0149 0.58 +0.7 162,258 + Psyr_0149 0.73 +0.5 162,259 - Psyr_0149 0.73 +0.2 162,597 + -0.2 162,597 + -0.4 162,598 - -0.7 162,598 - -0.5 162,608 + -0.9 162,680 - -2.6 162,763 - +1.5 162,763 - -0.1 162,927 + Psyr_0150 0.14 -0.1 162,928 - Psyr_0150 0.14 +2.0 162,928 - Psyr_0150 0.14 +0.5 163,076 + Psyr_0150 0.27 +0.7 163,076 + Psyr_0150 0.27 +0.4 163,077 - Psyr_0150 0.27 -0.3 163,217 + Psyr_0150 0.39 -0.1 163,218 - Psyr_0150 0.39 -0.3 163,218 - Psyr_0150 0.39 -1.6 163,218 - Psyr_0150 0.39 +0.3 163,218 - Psyr_0150 0.39 -0.1 163,227 + Psyr_0150 0.40 -0.3 163,227 + Psyr_0150 0.40 +0.5 163,227 + Psyr_0150 0.40 -0.6 163,457 + Psyr_0150 0.60 -0.1 163,458 - Psyr_0150 0.60 -0.2 163,458 - Psyr_0150 0.60 +0.7 163,629 + Psyr_0150 0.75 -0.6 163,629 + Psyr_0150 0.75 -0.6 163,630 - Psyr_0150 0.75 -1.1 163,630 - Psyr_0150 0.75 +0.4 163,630 - Psyr_0150 0.75 -1.6 163,630 - Psyr_0150 0.75 +0.3
Or see this region's nucleotide sequence