Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5753 and Pf1N1B4_5754 are separated by 28 nucleotides Pf1N1B4_5754 and Pf1N1B4_5755 overlap by 7 nucleotides Pf1N1B4_5755 and Pf1N1B4_5756 are separated by 592 nucleotides
Pf1N1B4_5753 - putative membrane protein, at 193,945 to 194,382
_5753
Pf1N1B4_5754 - NnrS protein involved in response to NO, at 194,411 to 195,073
_5754
Pf1N1B4_5755 - NnrS protein involved in response to NO, at 195,067 to 195,600
_5755
Pf1N1B4_5756 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 196,193 to 196,876
_5756
Position (kb) 195
196 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 194.079 kb on - strand, within Pf1N1B4_5753 at 194.079 kb on - strand, within Pf1N1B4_5753 at 194.199 kb on - strand, within Pf1N1B4_5753 at 194.272 kb on + strand at 194.470 kb on - strand at 194.911 kb on - strand, within Pf1N1B4_5754 at 194.912 kb on + strand, within Pf1N1B4_5754 at 194.913 kb on - strand, within Pf1N1B4_5754 at 195.171 kb on + strand, within Pf1N1B4_5755 at 195.172 kb on - strand, within Pf1N1B4_5755 at 195.172 kb on - strand, within Pf1N1B4_5755 at 195.172 kb on - strand, within Pf1N1B4_5755 at 195.172 kb on - strand, within Pf1N1B4_5755 at 195.615 kb on - strand at 195.655 kb on + strand at 195.656 kb on - strand at 195.656 kb on - strand at 195.926 kb on + strand at 195.927 kb on - strand at 196.142 kb on + strand at 196.352 kb on + strand, within Pf1N1B4_5756 at 196.353 kb on - strand, within Pf1N1B4_5756 at 196.479 kb on - strand, within Pf1N1B4_5756 at 196.538 kb on + strand, within Pf1N1B4_5756 at 196.539 kb on - strand, within Pf1N1B4_5756
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 194,079 - Pf1N1B4_5753 0.31 -0.9 194,079 - Pf1N1B4_5753 0.31 -0.3 194,199 - Pf1N1B4_5753 0.58 -2.9 194,272 + +2.1 194,470 - -1.4 194,911 - Pf1N1B4_5754 0.75 -0.9 194,912 + Pf1N1B4_5754 0.76 +0.4 194,913 - Pf1N1B4_5754 0.76 -1.0 195,171 + Pf1N1B4_5755 0.19 -2.2 195,172 - Pf1N1B4_5755 0.20 -1.1 195,172 - Pf1N1B4_5755 0.20 +0.8 195,172 - Pf1N1B4_5755 0.20 -1.8 195,172 - Pf1N1B4_5755 0.20 +1.7 195,615 - -1.7 195,655 + +0.4 195,656 - -0.5 195,656 - -0.5 195,926 + -0.6 195,927 - -1.2 196,142 + +0.3 196,352 + Pf1N1B4_5756 0.23 +0.5 196,353 - Pf1N1B4_5756 0.23 +0.8 196,479 - Pf1N1B4_5756 0.42 +0.1 196,538 + Pf1N1B4_5756 0.50 -0.2 196,539 - Pf1N1B4_5756 0.51 -0.2
Or see this region's nucleotide sequence