Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5410 and Pf1N1B4_5411 are separated by 0 nucleotides Pf1N1B4_5411 and Pf1N1B4_5412 are separated by 7 nucleotides
Pf1N1B4_5410 - hypothetical protein, at 144,116 to 146,206
_5410
Pf1N1B4_5411 - hypothetical protein, at 146,207 to 147,616
_5411
Pf1N1B4_5412 - hypothetical protein, at 147,624 to 148,703
_5412
Position (kb) 146
147
148 Strain fitness (log2 ratio) -2
-1
0
1
2 at 145.296 kb on + strand, within Pf1N1B4_5410 at 145.297 kb on - strand, within Pf1N1B4_5410 at 145.510 kb on - strand, within Pf1N1B4_5410 at 145.627 kb on + strand, within Pf1N1B4_5410 at 145.627 kb on + strand, within Pf1N1B4_5410 at 145.628 kb on - strand, within Pf1N1B4_5410 at 145.656 kb on + strand, within Pf1N1B4_5410 at 145.657 kb on - strand, within Pf1N1B4_5410 at 145.693 kb on - strand, within Pf1N1B4_5410 at 145.702 kb on - strand, within Pf1N1B4_5410 at 145.703 kb on + strand, within Pf1N1B4_5410 at 145.703 kb on + strand, within Pf1N1B4_5410 at 145.703 kb on + strand, within Pf1N1B4_5410 at 145.703 kb on + strand, within Pf1N1B4_5410 at 145.703 kb on + strand, within Pf1N1B4_5410 at 145.703 kb on + strand, within Pf1N1B4_5410 at 145.704 kb on - strand, within Pf1N1B4_5410 at 145.837 kb on + strand, within Pf1N1B4_5410 at 145.841 kb on + strand, within Pf1N1B4_5410 at 145.884 kb on + strand, within Pf1N1B4_5410 at 145.938 kb on + strand, within Pf1N1B4_5410 at 145.939 kb on - strand, within Pf1N1B4_5410 at 145.992 kb on - strand, within Pf1N1B4_5410 at 145.992 kb on - strand, within Pf1N1B4_5410 at 145.992 kb on - strand, within Pf1N1B4_5410 at 146.081 kb on - strand at 146.088 kb on - strand at 146.178 kb on - strand at 146.209 kb on - strand at 146.293 kb on + strand at 146.294 kb on - strand at 146.334 kb on + strand at 146.335 kb on - strand at 146.335 kb on - strand at 146.412 kb on - strand, within Pf1N1B4_5411 at 146.452 kb on - strand, within Pf1N1B4_5411 at 146.483 kb on - strand, within Pf1N1B4_5411 at 146.516 kb on - strand at 146.602 kb on - strand, within Pf1N1B4_5411 at 146.658 kb on - strand, within Pf1N1B4_5411 at 146.685 kb on + strand, within Pf1N1B4_5411 at 146.685 kb on + strand, within Pf1N1B4_5411 at 146.774 kb on + strand, within Pf1N1B4_5411 at 146.879 kb on + strand, within Pf1N1B4_5411 at 146.936 kb on - strand, within Pf1N1B4_5411 at 147.014 kb on - strand, within Pf1N1B4_5411 at 147.100 kb on + strand, within Pf1N1B4_5411 at 147.119 kb on - strand, within Pf1N1B4_5411 at 147.332 kb on + strand, within Pf1N1B4_5411 at 147.333 kb on - strand, within Pf1N1B4_5411 at 147.354 kb on + strand, within Pf1N1B4_5411 at 147.481 kb on + strand at 147.482 kb on - strand at 147.486 kb on + strand at 147.513 kb on - strand at 147.513 kb on - strand at 147.531 kb on + strand at 147.532 kb on - strand at 147.585 kb on - strand at 147.605 kb on - strand at 147.609 kb on - strand at 147.629 kb on + strand at 147.630 kb on - strand at 147.645 kb on - strand at 147.646 kb on + strand at 147.752 kb on + strand, within Pf1N1B4_5412 at 148.295 kb on + strand, within Pf1N1B4_5412 at 148.372 kb on + strand, within Pf1N1B4_5412
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 145,296 + Pf1N1B4_5410 0.56 +1.7 145,297 - Pf1N1B4_5410 0.56 -1.1 145,510 - Pf1N1B4_5410 0.67 -1.3 145,627 + Pf1N1B4_5410 0.72 -1.1 145,627 + Pf1N1B4_5410 0.72 +0.1 145,628 - Pf1N1B4_5410 0.72 -1.1 145,656 + Pf1N1B4_5410 0.74 -1.8 145,657 - Pf1N1B4_5410 0.74 +2.2 145,693 - Pf1N1B4_5410 0.75 +0.2 145,702 - Pf1N1B4_5410 0.76 -0.3 145,703 + Pf1N1B4_5410 0.76 -2.0 145,703 + Pf1N1B4_5410 0.76 -1.1 145,703 + Pf1N1B4_5410 0.76 -0.2 145,703 + Pf1N1B4_5410 0.76 -0.6 145,703 + Pf1N1B4_5410 0.76 +0.0 145,703 + Pf1N1B4_5410 0.76 -0.2 145,704 - Pf1N1B4_5410 0.76 +0.1 145,837 + Pf1N1B4_5410 0.82 +2.2 145,841 + Pf1N1B4_5410 0.82 +1.7 145,884 + Pf1N1B4_5410 0.85 -1.0 145,938 + Pf1N1B4_5410 0.87 -1.5 145,939 - Pf1N1B4_5410 0.87 -1.0 145,992 - Pf1N1B4_5410 0.90 +1.2 145,992 - Pf1N1B4_5410 0.90 -0.3 145,992 - Pf1N1B4_5410 0.90 +0.8 146,081 - +0.1 146,088 - -1.7 146,178 - +0.7 146,209 - +1.3 146,293 + +0.0 146,294 - +0.4 146,334 + -2.0 146,335 - +0.6 146,335 - +1.1 146,412 - Pf1N1B4_5411 0.15 +0.6 146,452 - Pf1N1B4_5411 0.17 +2.1 146,483 - Pf1N1B4_5411 0.20 -1.4 146,516 - +0.2 146,602 - Pf1N1B4_5411 0.28 -0.5 146,658 - Pf1N1B4_5411 0.32 +0.2 146,685 + Pf1N1B4_5411 0.34 +2.0 146,685 + Pf1N1B4_5411 0.34 -0.7 146,774 + Pf1N1B4_5411 0.40 -1.8 146,879 + Pf1N1B4_5411 0.48 +2.2 146,936 - Pf1N1B4_5411 0.52 +0.1 147,014 - Pf1N1B4_5411 0.57 +0.8 147,100 + Pf1N1B4_5411 0.63 +1.6 147,119 - Pf1N1B4_5411 0.65 -1.3 147,332 + Pf1N1B4_5411 0.80 +1.0 147,333 - Pf1N1B4_5411 0.80 -0.5 147,354 + Pf1N1B4_5411 0.81 -1.3 147,481 + +0.1 147,482 - -0.0 147,486 + -0.1 147,513 - +0.4 147,513 - -0.2 147,531 + +0.9 147,532 - -1.0 147,585 - -0.1 147,605 - +0.9 147,609 - -0.6 147,629 + +0.2 147,630 - +1.0 147,645 - -0.8 147,646 + +0.2 147,752 + Pf1N1B4_5412 0.12 -2.2 148,295 + Pf1N1B4_5412 0.62 +1.0 148,372 + Pf1N1B4_5412 0.69 +0.1
Or see this region's nucleotide sequence