Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3538 and Pf1N1B4_3539 are separated by 136 nucleotides Pf1N1B4_3539 and Pf1N1B4_3540 are separated by 72 nucleotides Pf1N1B4_3540 and Pf1N1B4_3541 are separated by 99 nucleotides
Pf1N1B4_3538 - Transcriptional regulator, AraC family, at 3,945,177 to 3,945,935
_3538
Pf1N1B4_3539 - Ser/Thr protein phosphatase family protein, UDP-2,3-diacylglucosamine hydrolase (EC 3.6.1.-) homolog, at 3,946,072 to 3,946,887
_3539
Pf1N1B4_3540 - Metal-dependent phosphohydrolase, HD subdomain, at 3,946,960 to 3,947,550
_3540
Pf1N1B4_3541 - FIG027190: Putative transmembrane protein, at 3,947,650 to 3,947,961
_3541
Position (kb)
3946
3947 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3945.130 kb on + strand at 3945.130 kb on + strand at 3945.136 kb on + strand at 3945.137 kb on - strand at 3945.155 kb on + strand at 3945.156 kb on - strand at 3945.156 kb on - strand at 3945.156 kb on - strand at 3945.352 kb on + strand, within Pf1N1B4_3538 at 3945.353 kb on - strand, within Pf1N1B4_3538 at 3945.353 kb on - strand at 3945.353 kb on - strand, within Pf1N1B4_3538 at 3945.460 kb on + strand, within Pf1N1B4_3538 at 3945.490 kb on + strand, within Pf1N1B4_3538 at 3945.490 kb on + strand, within Pf1N1B4_3538 at 3945.908 kb on - strand at 3946.104 kb on + strand at 3946.213 kb on + strand, within Pf1N1B4_3539 at 3946.213 kb on + strand, within Pf1N1B4_3539 at 3946.214 kb on - strand at 3946.214 kb on - strand, within Pf1N1B4_3539 at 3946.214 kb on - strand, within Pf1N1B4_3539 at 3946.214 kb on - strand, within Pf1N1B4_3539 at 3946.261 kb on + strand, within Pf1N1B4_3539 at 3946.261 kb on + strand, within Pf1N1B4_3539 at 3946.261 kb on + strand, within Pf1N1B4_3539 at 3946.261 kb on + strand at 3946.261 kb on + strand, within Pf1N1B4_3539 at 3946.261 kb on + strand, within Pf1N1B4_3539 at 3946.261 kb on + strand, within Pf1N1B4_3539 at 3946.261 kb on + strand, within Pf1N1B4_3539 at 3946.262 kb on - strand, within Pf1N1B4_3539 at 3946.262 kb on - strand, within Pf1N1B4_3539 at 3946.262 kb on - strand, within Pf1N1B4_3539 at 3946.262 kb on - strand, within Pf1N1B4_3539 at 3946.262 kb on - strand, within Pf1N1B4_3539 at 3946.262 kb on - strand at 3946.262 kb on - strand, within Pf1N1B4_3539 at 3946.262 kb on - strand, within Pf1N1B4_3539 at 3946.262 kb on - strand, within Pf1N1B4_3539 at 3946.262 kb on - strand, within Pf1N1B4_3539 at 3946.262 kb on - strand, within Pf1N1B4_3539 at 3946.262 kb on - strand, within Pf1N1B4_3539 at 3946.262 kb on - strand, within Pf1N1B4_3539 at 3946.279 kb on - strand, within Pf1N1B4_3539 at 3946.333 kb on + strand, within Pf1N1B4_3539 at 3946.333 kb on + strand, within Pf1N1B4_3539 at 3946.333 kb on + strand, within Pf1N1B4_3539 at 3946.333 kb on + strand, within Pf1N1B4_3539 at 3946.334 kb on - strand, within Pf1N1B4_3539 at 3946.334 kb on - strand, within Pf1N1B4_3539 at 3946.334 kb on - strand at 3946.534 kb on - strand, within Pf1N1B4_3539 at 3946.583 kb on - strand, within Pf1N1B4_3539 at 3946.600 kb on + strand at 3946.600 kb on + strand, within Pf1N1B4_3539 at 3946.747 kb on + strand, within Pf1N1B4_3539 at 3946.747 kb on + strand at 3946.819 kb on + strand at 3946.819 kb on + strand at 3946.866 kb on + strand at 3946.866 kb on + strand at 3946.898 kb on + strand at 3946.898 kb on + strand at 3946.899 kb on - strand at 3946.899 kb on - strand at 3946.899 kb on - strand at 3946.958 kb on + strand at 3946.959 kb on - strand at 3946.959 kb on - strand at 3946.959 kb on - strand at 3946.973 kb on + strand at 3946.974 kb on - strand at 3946.974 kb on - strand at 3947.123 kb on + strand at 3947.186 kb on + strand, within Pf1N1B4_3540 at 3947.186 kb on + strand, within Pf1N1B4_3540 at 3947.186 kb on + strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.190 kb on - strand, within Pf1N1B4_3540 at 3947.282 kb on + strand, within Pf1N1B4_3540 at 3947.330 kb on + strand, within Pf1N1B4_3540 at 3947.330 kb on + strand at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand at 3947.425 kb on + strand, within Pf1N1B4_3540 at 3947.523 kb on + strand at 3947.524 kb on - strand at 3947.592 kb on + strand at 3947.652 kb on - strand at 3947.652 kb on - strand at 3947.684 kb on + strand, within Pf1N1B4_3541 at 3947.685 kb on - strand, within Pf1N1B4_3541 at 3947.685 kb on - strand, within Pf1N1B4_3541 at 3947.778 kb on + strand at 3947.778 kb on + strand, within Pf1N1B4_3541 at 3947.778 kb on + strand, within Pf1N1B4_3541 at 3947.778 kb on + strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 3,945,130 + -0.7 3,945,130 + -1.9 3,945,136 + +0.5 3,945,137 - +0.9 3,945,155 + -0.9 3,945,156 - -0.1 3,945,156 - -1.1 3,945,156 - +1.1 3,945,352 + Pf1N1B4_3538 0.23 -1.7 3,945,353 - Pf1N1B4_3538 0.23 -0.6 3,945,353 - +0.4 3,945,353 - Pf1N1B4_3538 0.23 -0.3 3,945,460 + Pf1N1B4_3538 0.37 -1.8 3,945,490 + Pf1N1B4_3538 0.41 +1.6 3,945,490 + Pf1N1B4_3538 0.41 +0.5 3,945,908 - +0.2 3,946,104 + -0.8 3,946,213 + Pf1N1B4_3539 0.17 -0.4 3,946,213 + Pf1N1B4_3539 0.17 +0.3 3,946,214 - -0.2 3,946,214 - Pf1N1B4_3539 0.17 +0.4 3,946,214 - Pf1N1B4_3539 0.17 -1.1 3,946,214 - Pf1N1B4_3539 0.17 +0.8 3,946,261 + Pf1N1B4_3539 0.23 -1.1 3,946,261 + Pf1N1B4_3539 0.23 -2.1 3,946,261 + Pf1N1B4_3539 0.23 +0.0 3,946,261 + +0.1 3,946,261 + Pf1N1B4_3539 0.23 -0.1 3,946,261 + Pf1N1B4_3539 0.23 +0.0 3,946,261 + Pf1N1B4_3539 0.23 -0.9 3,946,261 + Pf1N1B4_3539 0.23 -0.2 3,946,262 - Pf1N1B4_3539 0.23 +0.8 3,946,262 - Pf1N1B4_3539 0.23 -1.4 3,946,262 - Pf1N1B4_3539 0.23 -2.6 3,946,262 - Pf1N1B4_3539 0.23 +0.7 3,946,262 - Pf1N1B4_3539 0.23 -0.7 3,946,262 - +4.4 3,946,262 - Pf1N1B4_3539 0.23 -1.5 3,946,262 - Pf1N1B4_3539 0.23 +0.9 3,946,262 - Pf1N1B4_3539 0.23 -1.1 3,946,262 - Pf1N1B4_3539 0.23 +0.6 3,946,262 - Pf1N1B4_3539 0.23 +0.2 3,946,262 - Pf1N1B4_3539 0.23 -0.3 3,946,262 - Pf1N1B4_3539 0.23 -1.7 3,946,279 - Pf1N1B4_3539 0.25 +0.0 3,946,333 + Pf1N1B4_3539 0.32 -0.6 3,946,333 + Pf1N1B4_3539 0.32 +0.3 3,946,333 + Pf1N1B4_3539 0.32 +0.1 3,946,333 + Pf1N1B4_3539 0.32 +1.6 3,946,334 - Pf1N1B4_3539 0.32 -0.5 3,946,334 - Pf1N1B4_3539 0.32 +0.2 3,946,334 - -1.6 3,946,534 - Pf1N1B4_3539 0.57 -3.6 3,946,583 - Pf1N1B4_3539 0.63 +1.6 3,946,600 + +0.2 3,946,600 + Pf1N1B4_3539 0.65 +0.2 3,946,747 + Pf1N1B4_3539 0.83 -0.2 3,946,747 + -0.6 3,946,819 + +1.2 3,946,819 + +2.0 3,946,866 + +1.6 3,946,866 + -1.0 3,946,898 + +0.7 3,946,898 + +0.4 3,946,899 - +0.2 3,946,899 - +0.1 3,946,899 - +1.7 3,946,958 + -0.3 3,946,959 - +0.7 3,946,959 - +0.2 3,946,959 - +0.4 3,946,973 + +1.0 3,946,974 - +0.8 3,946,974 - +0.2 3,947,123 + -1.4 3,947,186 + Pf1N1B4_3540 0.38 +0.0 3,947,186 + Pf1N1B4_3540 0.38 -0.8 3,947,186 + Pf1N1B4_3540 0.38 -0.5 3,947,187 - Pf1N1B4_3540 0.38 -0.5 3,947,187 - Pf1N1B4_3540 0.38 -0.1 3,947,187 - Pf1N1B4_3540 0.38 +0.9 3,947,187 - Pf1N1B4_3540 0.38 -1.5 3,947,190 - Pf1N1B4_3540 0.39 +1.6 3,947,282 + Pf1N1B4_3540 0.54 +0.1 3,947,330 + Pf1N1B4_3540 0.63 +0.7 3,947,330 + -1.8 3,947,331 - Pf1N1B4_3540 0.63 +1.0 3,947,331 - Pf1N1B4_3540 0.63 +0.2 3,947,331 - Pf1N1B4_3540 0.63 +0.2 3,947,331 - Pf1N1B4_3540 0.63 +1.7 3,947,331 - Pf1N1B4_3540 0.63 +0.6 3,947,331 - Pf1N1B4_3540 0.63 +1.8 3,947,331 - +1.8 3,947,425 + Pf1N1B4_3540 0.79 -0.9 3,947,523 + +1.4 3,947,524 - -0.8 3,947,592 + -0.7 3,947,652 - -0.8 3,947,652 - -0.7 3,947,684 + Pf1N1B4_3541 0.11 -0.5 3,947,685 - Pf1N1B4_3541 0.11 -1.4 3,947,685 - Pf1N1B4_3541 0.11 -1.4 3,947,778 + -1.1 3,947,778 + Pf1N1B4_3541 0.41 +0.3 3,947,778 + Pf1N1B4_3541 0.41 -1.8 3,947,778 + Pf1N1B4_3541 0.41 -1.1 3,947,779 - Pf1N1B4_3541 0.41 -0.9 3,947,779 - Pf1N1B4_3541 0.41 -0.3 3,947,779 - Pf1N1B4_3541 0.41 -2.1 3,947,779 - Pf1N1B4_3541 0.41 -1.4
Or see this region's nucleotide sequence