Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3123 and Pf1N1B4_3124 are separated by 67 nucleotides Pf1N1B4_3124 and Pf1N1B4_3125 overlap by 4 nucleotides Pf1N1B4_3125 and Pf1N1B4_3126 are separated by 127 nucleotides Pf1N1B4_3126 and Pf1N1B4_3127 overlap by 14 nucleotides
Pf1N1B4_3123 - Bacteriophage tail sheath protein, at 3,508,588 to 3,510,084
_3123
Pf1N1B4_3124 - Phage tail tube protein, at 3,510,152 to 3,510,499
_3124
Pf1N1B4_3125 - FIG00954242: hypothetical protein, at 3,510,496 to 3,510,792
_3125
Pf1N1B4_3126 - Phage tail length tape-measure protein, at 3,510,920 to 3,512,692
_3126
Pf1N1B4_3127 - Phage tail/DNA circulation protein, at 3,512,679 to 3,513,917
_3127
Position (kb)
3510
3511
3512
3513 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3510.016 kb on + strand at 3510.016 kb on + strand at 3510.016 kb on + strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.067 kb on + strand at 3510.067 kb on + strand at 3510.067 kb on + strand at 3510.067 kb on + strand at 3510.068 kb on - strand at 3510.132 kb on + strand at 3510.132 kb on + strand at 3510.133 kb on - strand at 3510.133 kb on - strand at 3510.179 kb on + strand at 3510.179 kb on + strand at 3510.179 kb on + strand at 3510.180 kb on - strand at 3510.209 kb on - strand, within Pf1N1B4_3124 at 3510.272 kb on + strand, within Pf1N1B4_3124 at 3510.272 kb on + strand, within Pf1N1B4_3124 at 3510.272 kb on + strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.398 kb on + strand, within Pf1N1B4_3124 at 3510.416 kb on + strand, within Pf1N1B4_3124 at 3510.611 kb on - strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.665 kb on - strand, within Pf1N1B4_3125 at 3510.665 kb on - strand, within Pf1N1B4_3125 at 3510.665 kb on - strand, within Pf1N1B4_3125 at 3510.805 kb on - strand at 3510.805 kb on - strand at 3510.805 kb on - strand at 3511.063 kb on + strand at 3511.422 kb on + strand, within Pf1N1B4_3126 at 3511.422 kb on + strand, within Pf1N1B4_3126 at 3511.422 kb on + strand, within Pf1N1B4_3126 at 3511.756 kb on + strand, within Pf1N1B4_3126 at 3511.756 kb on + strand, within Pf1N1B4_3126 at 3511.756 kb on + strand, within Pf1N1B4_3126 at 3511.964 kb on - strand, within Pf1N1B4_3126 at 3511.964 kb on - strand, within Pf1N1B4_3126 at 3511.964 kb on - strand at 3511.964 kb on - strand, within Pf1N1B4_3126 at 3512.126 kb on + strand, within Pf1N1B4_3126 at 3513.198 kb on + strand, within Pf1N1B4_3127 at 3513.199 kb on - strand, within Pf1N1B4_3127 at 3513.199 kb on - strand, within Pf1N1B4_3127 at 3513.199 kb on - strand, within Pf1N1B4_3127 at 3513.324 kb on - strand, within Pf1N1B4_3127 at 3513.357 kb on + strand, within Pf1N1B4_3127 at 3513.690 kb on + strand, within Pf1N1B4_3127
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 3,510,016 + +0.6 3,510,016 + -0.0 3,510,016 + +0.9 3,510,017 - -0.3 3,510,017 - -0.5 3,510,017 - +1.6 3,510,017 - +1.6 3,510,017 - -0.1 3,510,067 + +1.3 3,510,067 + -1.1 3,510,067 + -1.8 3,510,067 + +1.7 3,510,068 - -1.1 3,510,132 + +2.6 3,510,132 + +1.6 3,510,133 - +0.8 3,510,133 - -0.9 3,510,179 + +1.6 3,510,179 + -1.2 3,510,179 + -0.2 3,510,180 - +0.7 3,510,209 - Pf1N1B4_3124 0.16 -1.2 3,510,272 + Pf1N1B4_3124 0.34 +1.4 3,510,272 + Pf1N1B4_3124 0.34 -2.0 3,510,272 + Pf1N1B4_3124 0.34 -0.8 3,510,273 - Pf1N1B4_3124 0.35 -2.2 3,510,273 - Pf1N1B4_3124 0.35 -1.4 3,510,273 - Pf1N1B4_3124 0.35 -1.6 3,510,273 - Pf1N1B4_3124 0.35 -0.4 3,510,273 - Pf1N1B4_3124 0.35 +0.7 3,510,273 - Pf1N1B4_3124 0.35 +1.4 3,510,273 - Pf1N1B4_3124 0.35 +0.3 3,510,398 + Pf1N1B4_3124 0.71 -0.3 3,510,416 + Pf1N1B4_3124 0.76 +0.0 3,510,611 - Pf1N1B4_3125 0.39 -0.1 3,510,664 + Pf1N1B4_3125 0.57 -1.3 3,510,664 + Pf1N1B4_3125 0.57 -0.2 3,510,664 + Pf1N1B4_3125 0.57 +0.2 3,510,664 + Pf1N1B4_3125 0.57 +0.2 3,510,664 + Pf1N1B4_3125 0.57 -0.5 3,510,664 + Pf1N1B4_3125 0.57 -2.4 3,510,665 - Pf1N1B4_3125 0.57 +0.7 3,510,665 - Pf1N1B4_3125 0.57 -1.4 3,510,665 - Pf1N1B4_3125 0.57 -1.2 3,510,805 - +0.1 3,510,805 - +0.5 3,510,805 - +1.4 3,511,063 + -1.9 3,511,422 + Pf1N1B4_3126 0.28 +1.7 3,511,422 + Pf1N1B4_3126 0.28 -0.4 3,511,422 + Pf1N1B4_3126 0.28 +0.5 3,511,756 + Pf1N1B4_3126 0.47 -0.7 3,511,756 + Pf1N1B4_3126 0.47 -2.0 3,511,756 + Pf1N1B4_3126 0.47 +1.2 3,511,964 - Pf1N1B4_3126 0.59 -0.9 3,511,964 - Pf1N1B4_3126 0.59 +0.1 3,511,964 - -0.4 3,511,964 - Pf1N1B4_3126 0.59 +0.2 3,512,126 + Pf1N1B4_3126 0.68 +0.9 3,513,198 + Pf1N1B4_3127 0.42 +1.0 3,513,199 - Pf1N1B4_3127 0.42 -0.0 3,513,199 - Pf1N1B4_3127 0.42 +2.4 3,513,199 - Pf1N1B4_3127 0.42 +1.0 3,513,324 - Pf1N1B4_3127 0.52 +0.9 3,513,357 + Pf1N1B4_3127 0.55 +0.7 3,513,690 + Pf1N1B4_3127 0.82 +1.2
Or see this region's nucleotide sequence