Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_148 and Pf1N1B4_149 overlap by 4 nucleotides Pf1N1B4_149 and Pf1N1B4_150 are separated by 304 nucleotides
Pf1N1B4_148 - Non-ribosomal peptide synthetase modules, pyoverdine @ Siderophore biosynthesis non-ribosomal peptide synthetase modules, at 182,592 to 188,378
_148
Pf1N1B4_149 - Pyoverdine sidechain non-ribosomal peptide synthetase PvdD, at 188,375 to 191,797
_149
Pf1N1B4_150 - SyrP-like protein, at 192,102 to 193,085
_150
Position (kb) 188
189
190
191
192 Strain fitness (log2 ratio) -2
-1
0
1 at 187.720 kb on + strand, within Pf1N1B4_148 at 188.378 kb on + strand at 188.379 kb on - strand at 188.379 kb on - strand at 188.691 kb on - strand at 188.691 kb on - strand at 188.886 kb on + strand, within Pf1N1B4_149 at 189.033 kb on + strand, within Pf1N1B4_149 at 189.180 kb on + strand, within Pf1N1B4_149 at 189.180 kb on + strand, within Pf1N1B4_149 at 189.180 kb on + strand at 189.530 kb on - strand, within Pf1N1B4_149 at 189.783 kb on + strand, within Pf1N1B4_149 at 189.783 kb on + strand, within Pf1N1B4_149 at 189.784 kb on - strand, within Pf1N1B4_149 at 189.813 kb on - strand, within Pf1N1B4_149 at 190.002 kb on + strand, within Pf1N1B4_149 at 190.002 kb on + strand, within Pf1N1B4_149 at 190.003 kb on - strand, within Pf1N1B4_149 at 190.003 kb on - strand, within Pf1N1B4_149 at 190.150 kb on - strand, within Pf1N1B4_149 at 190.413 kb on + strand, within Pf1N1B4_149 at 190.413 kb on + strand, within Pf1N1B4_149 at 191.193 kb on + strand, within Pf1N1B4_149 at 191.193 kb on + strand, within Pf1N1B4_149 at 191.194 kb on - strand, within Pf1N1B4_149 at 191.194 kb on - strand, within Pf1N1B4_149 at 191.584 kb on - strand at 191.584 kb on - strand at 191.637 kb on + strand at 191.637 kb on + strand at 191.637 kb on + strand at 191.638 kb on - strand at 191.883 kb on + strand at 191.906 kb on - strand at 192.305 kb on - strand, within Pf1N1B4_150 at 192.598 kb on + strand at 192.653 kb on - strand, within Pf1N1B4_150 at 192.653 kb on - strand, within Pf1N1B4_150 at 192.712 kb on + strand, within Pf1N1B4_150 at 192.713 kb on - strand, within Pf1N1B4_150 at 192.746 kb on - strand, within Pf1N1B4_150 at 192.746 kb on - strand, within Pf1N1B4_150 at 192.746 kb on - strand, within Pf1N1B4_150
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 187,720 + Pf1N1B4_148 0.89 +0.1 188,378 + +0.1 188,379 - -0.1 188,379 - -0.5 188,691 - +0.5 188,691 - +0.6 188,886 + Pf1N1B4_149 0.15 -0.9 189,033 + Pf1N1B4_149 0.19 -1.5 189,180 + Pf1N1B4_149 0.24 -0.3 189,180 + Pf1N1B4_149 0.24 +0.8 189,180 + -0.3 189,530 - Pf1N1B4_149 0.34 -1.5 189,783 + Pf1N1B4_149 0.41 +0.7 189,783 + Pf1N1B4_149 0.41 -0.7 189,784 - Pf1N1B4_149 0.41 +0.7 189,813 - Pf1N1B4_149 0.42 +0.5 190,002 + Pf1N1B4_149 0.48 -1.7 190,002 + Pf1N1B4_149 0.48 -2.1 190,003 - Pf1N1B4_149 0.48 -0.7 190,003 - Pf1N1B4_149 0.48 -2.1 190,150 - Pf1N1B4_149 0.52 -2.3 190,413 + Pf1N1B4_149 0.60 -0.8 190,413 + Pf1N1B4_149 0.60 +0.6 191,193 + Pf1N1B4_149 0.82 +0.4 191,193 + Pf1N1B4_149 0.82 -1.2 191,194 - Pf1N1B4_149 0.82 +0.1 191,194 - Pf1N1B4_149 0.82 -0.8 191,584 - +1.1 191,584 - -0.2 191,637 + -0.8 191,637 + +0.2 191,637 + +1.7 191,638 - +0.9 191,883 + +0.3 191,906 - -0.4 192,305 - Pf1N1B4_150 0.21 +0.3 192,598 + +1.3 192,653 - Pf1N1B4_150 0.56 +0.5 192,653 - Pf1N1B4_150 0.56 -1.1 192,712 + Pf1N1B4_150 0.62 +0.3 192,713 - Pf1N1B4_150 0.62 -2.0 192,746 - Pf1N1B4_150 0.65 -0.6 192,746 - Pf1N1B4_150 0.65 +0.4 192,746 - Pf1N1B4_150 0.65 -2.4
Or see this region's nucleotide sequence