Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1161 and Pf1N1B4_1162 are separated by 27 nucleotides Pf1N1B4_1162 and Pf1N1B4_1163 are separated by 47 nucleotides Pf1N1B4_1163 and Pf1N1B4_5176 are separated by 141 nucleotides Pf1N1B4_5176 and Pf1N1B4_5177 are separated by 99 nucleotides
Pf1N1B4_1161 - Translation initiation factor 2, at 1,307,495 to 1,310,020
_1161
Pf1N1B4_1162 - Transcription termination protein NusA, at 1,310,048 to 1,311,529
_1162
Pf1N1B4_1163 - FIG000325: clustered with transcription termination protein NusA, at 1,311,577 to 1,312,035
_1163
Pf1N1B4_5176 - tRNA-Met-CAT, at 1,312,177 to 1,312,250
_5176
Pf1N1B4_5177 - tRNA-Leu-GAG, at 1,312,350 to 1,312,432
_5177
Position (kb)
1310
1311
1312 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1310.046 kb on - strand at 1310.046 kb on - strand at 1310.050 kb on - strand at 1310.050 kb on - strand at 1310.166 kb on - strand at 1312.056 kb on - strand at 1312.056 kb on - strand at 1312.056 kb on - strand at 1312.056 kb on - strand at 1312.056 kb on - strand at 1312.056 kb on - strand at 1312.299 kb on - strand at 1312.299 kb on - strand at 1312.311 kb on - strand at 1312.311 kb on - strand at 1312.344 kb on - strand at 1312.436 kb on - strand at 1312.439 kb on - strand at 1312.439 kb on - strand at 1312.439 kb on - strand at 1312.439 kb on - strand at 1312.457 kb on - strand at 1312.457 kb on - strand at 1312.457 kb on - strand at 1312.457 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 1,310,046 - +1.0 1,310,046 - +0.4 1,310,050 - +0.3 1,310,050 - +1.8 1,310,166 - +1.6 1,312,056 - -2.1 1,312,056 - -0.0 1,312,056 - +0.3 1,312,056 - +0.3 1,312,056 - -1.5 1,312,056 - +0.2 1,312,299 - +0.5 1,312,299 - -0.6 1,312,311 - -1.0 1,312,311 - +0.7 1,312,344 - -0.5 1,312,436 - -0.9 1,312,439 - +0.4 1,312,439 - -0.5 1,312,439 - +0.9 1,312,439 - +1.0 1,312,457 - -0.2 1,312,457 - +0.2 1,312,457 - -1.7 1,312,457 - +0.8
Or see this region's nucleotide sequence