Experiment: BHIS with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4236 and BT4237 are separated by 47 nucleotides BT4237 and BT4238 are separated by 64 nucleotides BT4238 and BT4239 are separated by 10 nucleotides BT4239 and BT4240 are separated by 45 nucleotides
BT4236: BT4236 - two-component system sensor histidine kinase/response regulator, hybrid ('one-component system') (NCBI ptt file), at 5,576,267 to 5,580,424
BT4236
BT4237: BT4237 - tRNA pseudouridine synthase A (NCBI ptt file), at 5,580,472 to 5,581,281
BT4237
BT4238: BT4238 - putative permease (NCBI ptt file), at 5,581,346 to 5,582,245
BT4238
BT4239: BT4239 - hypothetical protein (NCBI ptt file), at 5,582,256 to 5,582,906
BT4239
BT4240: BT4240 - conserved hypothetical protein, with a phosphotransferase enzyme family domain (NCBI ptt file), at 5,582,952 to 5,584,043
BT4240
Position (kb)
5581
5582
5583 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5580.413 kb on - strand at 5580.416 kb on + strand at 5580.416 kb on + strand at 5580.417 kb on - strand at 5580.417 kb on - strand at 5580.417 kb on - strand at 5580.482 kb on - strand at 5580.799 kb on + strand, within BT4237 at 5580.801 kb on + strand, within BT4237 at 5580.802 kb on - strand, within BT4237 at 5580.805 kb on + strand, within BT4237 at 5580.808 kb on + strand, within BT4237 at 5580.817 kb on - strand, within BT4237 at 5580.860 kb on - strand, within BT4237 at 5580.875 kb on - strand, within BT4237 at 5580.946 kb on - strand, within BT4237 at 5580.953 kb on + strand, within BT4237 at 5581.026 kb on + strand, within BT4237 at 5581.027 kb on - strand, within BT4237 at 5581.133 kb on + strand, within BT4237 at 5581.248 kb on - strand at 5581.248 kb on - strand at 5581.251 kb on + strand at 5581.280 kb on - strand at 5581.280 kb on - strand at 5581.312 kb on + strand at 5581.313 kb on - strand at 5581.331 kb on - strand at 5581.331 kb on - strand at 5581.354 kb on - strand at 5581.399 kb on - strand at 5581.399 kb on - strand at 5581.535 kb on - strand, within BT4238 at 5581.568 kb on + strand, within BT4238 at 5581.568 kb on + strand, within BT4238 at 5581.569 kb on - strand, within BT4238 at 5581.569 kb on - strand, within BT4238 at 5581.573 kb on + strand, within BT4238 at 5581.589 kb on - strand, within BT4238 at 5581.642 kb on + strand, within BT4238 at 5581.689 kb on + strand, within BT4238 at 5581.690 kb on - strand, within BT4238 at 5581.728 kb on - strand, within BT4238 at 5581.832 kb on - strand, within BT4238 at 5581.871 kb on + strand, within BT4238 at 5581.871 kb on + strand, within BT4238 at 5581.924 kb on + strand, within BT4238 at 5581.924 kb on + strand, within BT4238 at 5581.925 kb on - strand, within BT4238 at 5582.198 kb on - strand at 5582.226 kb on + strand at 5582.227 kb on - strand at 5582.280 kb on - strand at 5582.280 kb on - strand at 5582.280 kb on - strand at 5582.351 kb on + strand, within BT4239 at 5582.493 kb on + strand, within BT4239 at 5582.539 kb on + strand, within BT4239 at 5582.556 kb on - strand, within BT4239 at 5582.582 kb on + strand, within BT4239 at 5582.622 kb on + strand, within BT4239 at 5582.625 kb on - strand, within BT4239 at 5582.715 kb on + strand, within BT4239 at 5582.752 kb on + strand, within BT4239 at 5582.753 kb on - strand, within BT4239 at 5582.753 kb on - strand, within BT4239 at 5582.783 kb on + strand, within BT4239 at 5582.817 kb on + strand, within BT4239 at 5582.819 kb on + strand, within BT4239 at 5582.819 kb on + strand, within BT4239 at 5582.820 kb on - strand, within BT4239 at 5582.875 kb on - strand at 5582.928 kb on + strand at 5583.000 kb on - strand at 5583.054 kb on - strand at 5583.054 kb on - strand at 5583.064 kb on + strand, within BT4240 at 5583.076 kb on + strand, within BT4240 at 5583.079 kb on + strand, within BT4240 at 5583.096 kb on - strand, within BT4240 at 5583.098 kb on - strand, within BT4240 at 5583.126 kb on - strand, within BT4240 at 5583.148 kb on + strand, within BT4240 at 5583.162 kb on + strand, within BT4240
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chenodeoxycholic 0.25 mg/ml remove 5,580,413 - -3.0 5,580,416 + -1.9 5,580,416 + +0.6 5,580,417 - -1.0 5,580,417 - -0.2 5,580,417 - +0.6 5,580,482 - -1.9 5,580,799 + BT4237 0.40 -0.4 5,580,801 + BT4237 0.41 -1.7 5,580,802 - BT4237 0.41 +0.5 5,580,805 + BT4237 0.41 -0.5 5,580,808 + BT4237 0.41 -1.4 5,580,817 - BT4237 0.43 +1.0 5,580,860 - BT4237 0.48 -3.4 5,580,875 - BT4237 0.50 -3.1 5,580,946 - BT4237 0.59 -1.5 5,580,953 + BT4237 0.59 -1.2 5,581,026 + BT4237 0.68 +0.6 5,581,027 - BT4237 0.69 +0.1 5,581,133 + BT4237 0.82 -1.9 5,581,248 - +0.6 5,581,248 - -1.4 5,581,251 + -1.2 5,581,280 - -0.9 5,581,280 - -1.4 5,581,312 + -1.7 5,581,313 - -0.4 5,581,331 - -2.3 5,581,331 - -0.4 5,581,354 - -1.7 5,581,399 - -2.2 5,581,399 - -0.4 5,581,535 - BT4238 0.21 -2.2 5,581,568 + BT4238 0.25 -2.2 5,581,568 + BT4238 0.25 -1.4 5,581,569 - BT4238 0.25 -2.9 5,581,569 - BT4238 0.25 -0.2 5,581,573 + BT4238 0.25 -3.6 5,581,589 - BT4238 0.27 -1.8 5,581,642 + BT4238 0.33 +0.1 5,581,689 + BT4238 0.38 -1.2 5,581,690 - BT4238 0.38 -0.4 5,581,728 - BT4238 0.42 -0.9 5,581,832 - BT4238 0.54 +0.6 5,581,871 + BT4238 0.58 -1.8 5,581,871 + BT4238 0.58 -1.0 5,581,924 + BT4238 0.64 -2.5 5,581,924 + BT4238 0.64 -1.9 5,581,925 - BT4238 0.64 -3.1 5,582,198 - -1.4 5,582,226 + -0.2 5,582,227 - -1.9 5,582,280 - +0.6 5,582,280 - -0.6 5,582,280 - -1.2 5,582,351 + BT4239 0.15 -0.7 5,582,493 + BT4239 0.36 -2.8 5,582,539 + BT4239 0.43 -2.3 5,582,556 - BT4239 0.46 -0.1 5,582,582 + BT4239 0.50 +0.0 5,582,622 + BT4239 0.56 +0.3 5,582,625 - BT4239 0.57 +1.5 5,582,715 + BT4239 0.71 +0.4 5,582,752 + BT4239 0.76 -1.9 5,582,753 - BT4239 0.76 -1.2 5,582,753 - BT4239 0.76 -1.6 5,582,783 + BT4239 0.81 +1.8 5,582,817 + BT4239 0.86 -0.4 5,582,819 + BT4239 0.86 +0.1 5,582,819 + BT4239 0.86 +0.8 5,582,820 - BT4239 0.87 -1.4 5,582,875 - -0.2 5,582,928 + -2.0 5,583,000 - -1.7 5,583,054 - +1.1 5,583,054 - -1.4 5,583,064 + BT4240 0.10 -0.3 5,583,076 + BT4240 0.11 +1.1 5,583,079 + BT4240 0.12 -0.9 5,583,096 - BT4240 0.13 +0.1 5,583,098 - BT4240 0.13 -1.9 5,583,126 - BT4240 0.16 -1.2 5,583,148 + BT4240 0.18 +0.3 5,583,162 + BT4240 0.19 -2.4
Or see this region's nucleotide sequence