Experiment: BHIS with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2580 and BT2581 are separated by 61 nucleotides BT2581 and BT2582 are separated by 104 nucleotides BT2582 and BT2583 are separated by 11 nucleotides BT2583 and BT2584 overlap by 8 nucleotides
BT2580: BT2580 - hypothetical protein (NCBI ptt file), at 3,224,933 to 3,225,151
BT2580
BT2581: BT2581 - hypothetical protein (NCBI ptt file), at 3,225,213 to 3,225,716
BT2581
BT2582: BT2582 - conserved hypothetical protein (NCBI ptt file), at 3,225,821 to 3,226,372
BT2582
BT2583: BT2583 - hypothetical protein (NCBI ptt file), at 3,226,384 to 3,227,310
BT2583
BT2584: BT2584 - hypothetical protein (NCBI ptt file), at 3,227,303 to 3,228,127
BT2584
Position (kb)
3225
3226
3227 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3224.836 kb on - strand at 3224.840 kb on + strand at 3224.864 kb on + strand at 3224.880 kb on - strand at 3224.882 kb on + strand at 3224.892 kb on + strand at 3224.893 kb on - strand at 3224.893 kb on - strand at 3224.901 kb on - strand at 3224.901 kb on - strand at 3224.910 kb on - strand at 3224.911 kb on + strand at 3224.963 kb on + strand, within BT2580 at 3224.964 kb on - strand, within BT2580 at 3224.997 kb on + strand, within BT2580 at 3224.998 kb on - strand, within BT2580 at 3225.022 kb on + strand, within BT2580 at 3225.154 kb on - strand at 3225.187 kb on + strand at 3225.207 kb on + strand at 3225.208 kb on - strand at 3225.254 kb on - strand at 3225.395 kb on + strand, within BT2581 at 3225.395 kb on + strand, within BT2581 at 3225.396 kb on - strand, within BT2581 at 3225.396 kb on - strand, within BT2581 at 3225.396 kb on - strand, within BT2581 at 3225.399 kb on + strand, within BT2581 at 3225.399 kb on + strand, within BT2581 at 3225.400 kb on - strand, within BT2581 at 3225.400 kb on - strand, within BT2581 at 3225.400 kb on - strand, within BT2581 at 3225.401 kb on + strand, within BT2581 at 3225.402 kb on - strand, within BT2581 at 3225.405 kb on + strand, within BT2581 at 3225.405 kb on + strand, within BT2581 at 3225.405 kb on + strand, within BT2581 at 3225.405 kb on + strand, within BT2581 at 3225.406 kb on - strand, within BT2581 at 3225.406 kb on - strand, within BT2581 at 3225.408 kb on - strand at 3225.413 kb on + strand, within BT2581 at 3225.418 kb on - strand, within BT2581 at 3225.535 kb on - strand, within BT2581 at 3225.535 kb on - strand, within BT2581 at 3225.970 kb on - strand, within BT2582 at 3226.026 kb on + strand, within BT2582 at 3226.058 kb on - strand, within BT2582 at 3226.058 kb on - strand, within BT2582 at 3226.058 kb on - strand, within BT2582 at 3226.177 kb on - strand, within BT2582 at 3226.370 kb on + strand at 3226.463 kb on + strand at 3226.474 kb on + strand at 3226.474 kb on + strand at 3226.477 kb on + strand, within BT2583 at 3226.477 kb on + strand, within BT2583 at 3226.550 kb on + strand, within BT2583 at 3226.619 kb on - strand, within BT2583 at 3226.621 kb on + strand, within BT2583 at 3226.624 kb on - strand, within BT2583 at 3226.624 kb on - strand, within BT2583 at 3226.630 kb on - strand, within BT2583 at 3226.702 kb on + strand, within BT2583 at 3226.703 kb on - strand, within BT2583 at 3226.703 kb on - strand, within BT2583 at 3226.703 kb on - strand, within BT2583 at 3226.703 kb on - strand, within BT2583 at 3226.703 kb on - strand, within BT2583 at 3226.851 kb on + strand, within BT2583 at 3226.852 kb on - strand, within BT2583 at 3226.994 kb on + strand, within BT2583 at 3226.995 kb on - strand, within BT2583 at 3227.027 kb on - strand, within BT2583 at 3227.027 kb on - strand, within BT2583 at 3227.093 kb on - strand, within BT2583 at 3227.100 kb on - strand, within BT2583 at 3227.102 kb on + strand, within BT2583 at 3227.201 kb on - strand, within BT2583 at 3227.226 kb on + strand at 3227.227 kb on - strand at 3227.227 kb on - strand at 3227.312 kb on - strand at 3227.331 kb on - strand at 3227.331 kb on - strand at 3227.355 kb on - strand at 3227.366 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chenodeoxycholic 0.25 mg/ml remove 3,224,836 - -1.2 3,224,840 + +1.0 3,224,864 + -0.4 3,224,880 - -1.2 3,224,882 + -2.5 3,224,892 + -1.0 3,224,893 - -0.1 3,224,893 - -2.8 3,224,901 - -0.6 3,224,901 - -2.2 3,224,910 - -0.1 3,224,911 + -0.6 3,224,963 + BT2580 0.14 +0.3 3,224,964 - BT2580 0.14 -3.7 3,224,997 + BT2580 0.29 +0.1 3,224,998 - BT2580 0.30 -0.5 3,225,022 + BT2580 0.41 +1.0 3,225,154 - -0.9 3,225,187 + -2.3 3,225,207 + -1.2 3,225,208 - -1.2 3,225,254 - +0.6 3,225,395 + BT2581 0.36 -1.2 3,225,395 + BT2581 0.36 -4.0 3,225,396 - BT2581 0.36 -0.2 3,225,396 - BT2581 0.36 +0.4 3,225,396 - BT2581 0.36 -1.6 3,225,399 + BT2581 0.37 -0.6 3,225,399 + BT2581 0.37 -1.8 3,225,400 - BT2581 0.37 -2.1 3,225,400 - BT2581 0.37 -0.2 3,225,400 - BT2581 0.37 -1.7 3,225,401 + BT2581 0.37 +0.4 3,225,402 - BT2581 0.38 +0.3 3,225,405 + BT2581 0.38 -1.2 3,225,405 + BT2581 0.38 -0.6 3,225,405 + BT2581 0.38 -0.0 3,225,405 + BT2581 0.38 +0.4 3,225,406 - BT2581 0.38 +0.8 3,225,406 - BT2581 0.38 -0.9 3,225,408 - +0.4 3,225,413 + BT2581 0.40 +0.0 3,225,418 - BT2581 0.41 +0.2 3,225,535 - BT2581 0.64 -0.2 3,225,535 - BT2581 0.64 +1.0 3,225,970 - BT2582 0.27 -2.8 3,226,026 + BT2582 0.37 -3.2 3,226,058 - BT2582 0.43 +0.3 3,226,058 - BT2582 0.43 -3.0 3,226,058 - BT2582 0.43 -1.2 3,226,177 - BT2582 0.64 -1.8 3,226,370 + -2.9 3,226,463 + -2.3 3,226,474 + +0.1 3,226,474 + +1.4 3,226,477 + BT2583 0.10 +0.4 3,226,477 + BT2583 0.10 -1.8 3,226,550 + BT2583 0.18 +0.1 3,226,619 - BT2583 0.25 -2.2 3,226,621 + BT2583 0.26 -1.2 3,226,624 - BT2583 0.26 -0.5 3,226,624 - BT2583 0.26 -2.9 3,226,630 - BT2583 0.27 +3.0 3,226,702 + BT2583 0.34 -0.0 3,226,703 - BT2583 0.34 +0.7 3,226,703 - BT2583 0.34 -0.9 3,226,703 - BT2583 0.34 -2.3 3,226,703 - BT2583 0.34 +1.8 3,226,703 - BT2583 0.34 -1.6 3,226,851 + BT2583 0.50 -1.6 3,226,852 - BT2583 0.50 -2.2 3,226,994 + BT2583 0.66 +0.3 3,226,995 - BT2583 0.66 +0.4 3,227,027 - BT2583 0.69 +1.0 3,227,027 - BT2583 0.69 -1.2 3,227,093 - BT2583 0.76 -1.6 3,227,100 - BT2583 0.77 +2.4 3,227,102 + BT2583 0.77 -2.4 3,227,201 - BT2583 0.88 -1.4 3,227,226 + -1.4 3,227,227 - -0.1 3,227,227 - +0.3 3,227,312 - -2.1 3,227,331 - -1.8 3,227,331 - -1.2 3,227,355 - -2.5 3,227,366 + -0.6
Or see this region's nucleotide sequence