Experiment: BHIS with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2294 and BT2295 are separated by 77 nucleotides BT2295 and BT2296 are separated by 6 nucleotides BT2296 and BT2297 are separated by 2 nucleotides BT2297 and BT2298 are separated by 623 nucleotides
BT2294: BT2294 - conserved protein found in conjugate transposon (NCBI ptt file), at 2,869,620 to 2,870,249
BT2294
BT2295: BT2295 - hypothetical protein (NCBI ptt file), at 2,870,327 to 2,870,599
BT2295
BT2296: BT2296 - conserved protein found in conjugate transposon (NCBI ptt file), at 2,870,606 to 2,871,208
BT2296
BT2297: BT2297 - putative reverse transcriptase (NCBI ptt file), at 2,871,211 to 2,872,869
BT2297
BT2298: BT2298 - conserved protein found in conjugate transposon (NCBI ptt file), at 2,873,493 to 2,875,520
BT2298
Position (kb)
2871
2872
2873 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2870.345 kb on + strand at 2870.349 kb on + strand at 2870.352 kb on - strand at 2870.481 kb on + strand, within BT2295 at 2870.499 kb on + strand, within BT2295 at 2870.503 kb on + strand, within BT2295 at 2870.571 kb on + strand, within BT2295 at 2870.587 kb on + strand at 2870.612 kb on + strand at 2870.891 kb on + strand, within BT2296 at 2871.115 kb on + strand, within BT2296 at 2871.115 kb on + strand, within BT2296 at 2871.116 kb on - strand, within BT2296 at 2871.212 kb on + strand at 2871.265 kb on - strand at 2871.371 kb on + strand at 2871.403 kb on + strand, within BT2297 at 2871.404 kb on - strand, within BT2297 at 2871.506 kb on + strand, within BT2297 at 2871.506 kb on + strand, within BT2297 at 2871.506 kb on + strand, within BT2297 at 2871.507 kb on - strand, within BT2297 at 2871.614 kb on + strand, within BT2297 at 2871.719 kb on + strand, within BT2297 at 2871.719 kb on + strand, within BT2297 at 2871.757 kb on + strand, within BT2297 at 2871.758 kb on - strand, within BT2297 at 2872.035 kb on - strand, within BT2297 at 2872.039 kb on + strand, within BT2297 at 2872.039 kb on + strand, within BT2297 at 2872.040 kb on - strand, within BT2297 at 2872.457 kb on + strand, within BT2297 at 2872.458 kb on - strand, within BT2297 at 2872.498 kb on + strand, within BT2297 at 2872.530 kb on + strand, within BT2297 at 2872.559 kb on + strand, within BT2297 at 2872.772 kb on - strand at 2872.808 kb on - strand at 2872.808 kb on - strand at 2872.831 kb on - strand at 2872.887 kb on + strand at 2872.887 kb on + strand at 2872.950 kb on + strand at 2872.965 kb on + strand at 2873.035 kb on + strand at 2873.077 kb on + strand at 2873.077 kb on + strand at 2873.078 kb on - strand at 2873.078 kb on - strand at 2873.091 kb on + strand at 2873.092 kb on - strand at 2873.111 kb on + strand at 2873.139 kb on + strand at 2873.149 kb on + strand at 2873.155 kb on + strand at 2873.253 kb on + strand at 2873.264 kb on + strand at 2873.276 kb on + strand at 2873.386 kb on + strand at 2873.404 kb on + strand at 2873.487 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chenodeoxycholic 0.25 mg/ml remove 2,870,345 + -1.0 2,870,349 + -1.6 2,870,352 - -0.7 2,870,481 + BT2295 0.56 -1.9 2,870,499 + BT2295 0.63 -1.0 2,870,503 + BT2295 0.64 -1.0 2,870,571 + BT2295 0.89 -2.0 2,870,587 + -0.2 2,870,612 + -0.8 2,870,891 + BT2296 0.47 -2.0 2,871,115 + BT2296 0.84 +0.7 2,871,115 + BT2296 0.84 -0.3 2,871,116 - BT2296 0.85 -0.5 2,871,212 + -2.0 2,871,265 - -1.0 2,871,371 + +0.3 2,871,403 + BT2297 0.12 -3.4 2,871,404 - BT2297 0.12 -1.7 2,871,506 + BT2297 0.18 +0.3 2,871,506 + BT2297 0.18 +0.4 2,871,506 + BT2297 0.18 -0.6 2,871,507 - BT2297 0.18 -1.5 2,871,614 + BT2297 0.24 +0.1 2,871,719 + BT2297 0.31 -1.8 2,871,719 + BT2297 0.31 -2.5 2,871,757 + BT2297 0.33 -2.4 2,871,758 - BT2297 0.33 -2.0 2,872,035 - BT2297 0.50 -1.7 2,872,039 + BT2297 0.50 -0.3 2,872,039 + BT2297 0.50 -0.7 2,872,040 - BT2297 0.50 -0.7 2,872,457 + BT2297 0.75 -2.4 2,872,458 - BT2297 0.75 +0.2 2,872,498 + BT2297 0.78 -1.3 2,872,530 + BT2297 0.80 +0.9 2,872,559 + BT2297 0.81 -2.1 2,872,772 - -0.1 2,872,808 - -0.7 2,872,808 - -0.7 2,872,831 - -1.0 2,872,887 + -3.2 2,872,887 + +0.3 2,872,950 + -1.5 2,872,965 + +0.9 2,873,035 + -0.6 2,873,077 + +0.3 2,873,077 + -2.0 2,873,078 - -1.9 2,873,078 - -1.5 2,873,091 + +0.3 2,873,092 - -0.3 2,873,111 + -0.7 2,873,139 + -0.5 2,873,149 + -1.9 2,873,155 + -1.3 2,873,253 + -0.4 2,873,264 + -0.3 2,873,276 + -1.9 2,873,386 + -1.3 2,873,404 + -0.7 2,873,487 + -0.7
Or see this region's nucleotide sequence