Experiment: KB with Esculin Monohydrate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2085 and Psyr_2086 are separated by 159 nucleotides Psyr_2086 and Psyr_2087 are separated by 68 nucleotides
Psyr_2085: Psyr_2085 - methylisocitrate lyase, at 2,430,393 to 2,431,286
_2085
Psyr_2086: Psyr_2086 - 2-methylcitrate synthase, at 2,431,446 to 2,432,573
_2086
Psyr_2087: Psyr_2087 - aconitase / 2-methylcitrate dehydratase (trans-methylaconitate-forming), at 2,432,642 to 2,435,230
_2087
Position (kb)
2431
2432
2433 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2430.521 kb on + strand, within Psyr_2085 at 2430.522 kb on - strand, within Psyr_2085 at 2430.525 kb on + strand, within Psyr_2085 at 2430.525 kb on + strand, within Psyr_2085 at 2430.525 kb on + strand, within Psyr_2085 at 2430.525 kb on + strand, within Psyr_2085 at 2430.525 kb on + strand, within Psyr_2085 at 2430.526 kb on - strand, within Psyr_2085 at 2430.526 kb on - strand, within Psyr_2085 at 2430.598 kb on + strand, within Psyr_2085 at 2430.599 kb on - strand, within Psyr_2085 at 2430.599 kb on - strand, within Psyr_2085 at 2430.930 kb on - strand, within Psyr_2085 at 2430.948 kb on - strand, within Psyr_2085 at 2430.984 kb on + strand, within Psyr_2085 at 2430.984 kb on + strand, within Psyr_2085 at 2430.984 kb on + strand, within Psyr_2085 at 2430.985 kb on - strand, within Psyr_2085 at 2431.059 kb on - strand, within Psyr_2085 at 2431.218 kb on + strand at 2431.219 kb on - strand at 2431.219 kb on - strand at 2431.352 kb on + strand at 2431.352 kb on + strand at 2431.353 kb on - strand at 2431.382 kb on + strand at 2431.462 kb on + strand at 2431.545 kb on + strand at 2431.602 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.653 kb on - strand, within Psyr_2086 at 2432.065 kb on - strand, within Psyr_2086 at 2432.236 kb on - strand, within Psyr_2086 at 2432.385 kb on + strand, within Psyr_2086 at 2432.385 kb on + strand, within Psyr_2086 at 2432.386 kb on - strand, within Psyr_2086 at 2432.526 kb on + strand at 2432.526 kb on + strand at 2432.526 kb on + strand at 2432.527 kb on - strand at 2432.571 kb on + strand at 2432.688 kb on - strand at 2432.732 kb on + strand at 2432.747 kb on + strand at 2432.747 kb on + strand at 2432.747 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Esculin Monohydrate 10 mM remove 2,430,521 + Psyr_2085 0.14 +1.6 2,430,522 - Psyr_2085 0.14 +0.3 2,430,525 + Psyr_2085 0.15 +1.5 2,430,525 + Psyr_2085 0.15 +1.5 2,430,525 + Psyr_2085 0.15 -0.5 2,430,525 + Psyr_2085 0.15 -0.4 2,430,525 + Psyr_2085 0.15 -0.8 2,430,526 - Psyr_2085 0.15 +1.2 2,430,526 - Psyr_2085 0.15 -0.8 2,430,598 + Psyr_2085 0.23 -0.3 2,430,599 - Psyr_2085 0.23 -1.3 2,430,599 - Psyr_2085 0.23 -0.1 2,430,930 - Psyr_2085 0.60 -0.1 2,430,948 - Psyr_2085 0.62 +0.0 2,430,984 + Psyr_2085 0.66 +1.3 2,430,984 + Psyr_2085 0.66 +0.4 2,430,984 + Psyr_2085 0.66 +1.0 2,430,985 - Psyr_2085 0.66 -2.5 2,431,059 - Psyr_2085 0.74 +1.2 2,431,218 + -1.1 2,431,219 - +0.8 2,431,219 - +1.0 2,431,352 + -0.7 2,431,352 + +0.5 2,431,353 - +1.3 2,431,382 + +1.4 2,431,462 + -0.7 2,431,545 + +0.0 2,431,602 + Psyr_2086 0.14 +0.5 2,431,650 + Psyr_2086 0.18 +0.3 2,431,650 + Psyr_2086 0.18 -1.4 2,431,650 + Psyr_2086 0.18 +1.0 2,431,650 + Psyr_2086 0.18 +0.8 2,431,650 + Psyr_2086 0.18 +0.5 2,431,650 + Psyr_2086 0.18 -0.4 2,431,651 - Psyr_2086 0.18 -1.4 2,431,651 - Psyr_2086 0.18 -0.3 2,431,651 - Psyr_2086 0.18 +1.3 2,431,651 - Psyr_2086 0.18 -1.8 2,431,651 - Psyr_2086 0.18 +1.0 2,431,651 - Psyr_2086 0.18 -1.2 2,431,653 - Psyr_2086 0.18 +0.5 2,432,065 - Psyr_2086 0.55 +0.1 2,432,236 - Psyr_2086 0.70 -0.7 2,432,385 + Psyr_2086 0.83 +0.8 2,432,385 + Psyr_2086 0.83 -0.3 2,432,386 - Psyr_2086 0.83 -0.6 2,432,526 + -2.1 2,432,526 + -0.8 2,432,526 + -1.5 2,432,527 - -0.9 2,432,571 + +0.5 2,432,688 - +2.4 2,432,732 + -2.3 2,432,747 + -0.5 2,432,747 + +1.0 2,432,747 + +1.1
Or see this region's nucleotide sequence