Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_755 and Pf1N1B4_756 are separated by 175 nucleotides Pf1N1B4_756 and Pf1N1B4_757 are separated by 274 nucleotides
Pf1N1B4_755 - RecA-family ATPase, at 858,442 to 859,923
_755
Pf1N1B4_756 - hypothetical protein, at 860,099 to 860,287
_756
Pf1N1B4_757 - phage-related integrase, at 860,562 to 861,812
_757
Position (kb)
860
861 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 859.237 kb on + strand, within Pf1N1B4_755 at 859.237 kb on + strand, within Pf1N1B4_755 at 859.392 kb on + strand, within Pf1N1B4_755 at 859.392 kb on + strand, within Pf1N1B4_755 at 859.392 kb on + strand at 859.392 kb on + strand, within Pf1N1B4_755 at 859.392 kb on + strand, within Pf1N1B4_755 at 859.392 kb on + strand, within Pf1N1B4_755 at 859.392 kb on + strand, within Pf1N1B4_755 at 859.393 kb on - strand, within Pf1N1B4_755 at 859.393 kb on - strand, within Pf1N1B4_755 at 859.393 kb on - strand, within Pf1N1B4_755 at 859.393 kb on - strand, within Pf1N1B4_755 at 859.393 kb on - strand, within Pf1N1B4_755 at 859.393 kb on - strand, within Pf1N1B4_755 at 859.396 kb on + strand, within Pf1N1B4_755 at 859.396 kb on + strand, within Pf1N1B4_755 at 859.396 kb on + strand, within Pf1N1B4_755 at 859.396 kb on + strand, within Pf1N1B4_755 at 859.397 kb on - strand, within Pf1N1B4_755 at 859.397 kb on - strand, within Pf1N1B4_755 at 859.397 kb on - strand, within Pf1N1B4_755 at 859.397 kb on - strand, within Pf1N1B4_755 at 859.450 kb on + strand, within Pf1N1B4_755 at 859.450 kb on + strand, within Pf1N1B4_755 at 859.450 kb on + strand, within Pf1N1B4_755 at 859.450 kb on + strand, within Pf1N1B4_755 at 859.451 kb on - strand, within Pf1N1B4_755 at 859.451 kb on - strand, within Pf1N1B4_755 at 859.451 kb on - strand, within Pf1N1B4_755 at 859.634 kb on + strand, within Pf1N1B4_755 at 859.634 kb on + strand, within Pf1N1B4_755 at 859.634 kb on + strand, within Pf1N1B4_755 at 859.635 kb on - strand, within Pf1N1B4_755 at 859.635 kb on - strand, within Pf1N1B4_755 at 859.635 kb on - strand, within Pf1N1B4_755 at 859.635 kb on - strand, within Pf1N1B4_755 at 859.635 kb on - strand at 859.635 kb on - strand, within Pf1N1B4_755 at 859.658 kb on + strand, within Pf1N1B4_755 at 859.658 kb on + strand at 859.658 kb on + strand, within Pf1N1B4_755 at 859.658 kb on + strand, within Pf1N1B4_755 at 859.658 kb on + strand, within Pf1N1B4_755 at 859.658 kb on + strand, within Pf1N1B4_755 at 859.658 kb on + strand, within Pf1N1B4_755 at 859.658 kb on + strand, within Pf1N1B4_755 at 859.658 kb on + strand, within Pf1N1B4_755 at 859.659 kb on + strand at 859.659 kb on - strand, within Pf1N1B4_755 at 859.659 kb on - strand, within Pf1N1B4_755 at 859.659 kb on - strand, within Pf1N1B4_755 at 859.659 kb on - strand, within Pf1N1B4_755 at 859.659 kb on - strand, within Pf1N1B4_755 at 859.659 kb on - strand, within Pf1N1B4_755 at 859.662 kb on + strand, within Pf1N1B4_755 at 859.673 kb on + strand at 859.673 kb on + strand, within Pf1N1B4_755 at 859.673 kb on + strand, within Pf1N1B4_755 at 859.673 kb on + strand, within Pf1N1B4_755 at 859.673 kb on + strand, within Pf1N1B4_755 at 859.674 kb on - strand, within Pf1N1B4_755 at 859.674 kb on - strand, within Pf1N1B4_755 at 859.674 kb on - strand, within Pf1N1B4_755 at 859.674 kb on - strand, within Pf1N1B4_755 at 859.674 kb on - strand at 859.674 kb on - strand, within Pf1N1B4_755 at 859.687 kb on + strand, within Pf1N1B4_755 at 859.739 kb on + strand, within Pf1N1B4_755 at 859.745 kb on + strand, within Pf1N1B4_755 at 859.745 kb on + strand, within Pf1N1B4_755 at 859.745 kb on + strand, within Pf1N1B4_755 at 859.745 kb on + strand, within Pf1N1B4_755 at 859.746 kb on - strand, within Pf1N1B4_755 at 859.746 kb on - strand, within Pf1N1B4_755 at 859.746 kb on - strand, within Pf1N1B4_755 at 859.814 kb on + strand at 859.815 kb on - strand at 859.815 kb on - strand at 859.903 kb on + strand at 859.903 kb on + strand at 859.904 kb on - strand at 859.904 kb on - strand at 859.923 kb on + strand at 859.923 kb on + strand at 859.923 kb on + strand at 859.923 kb on + strand at 859.923 kb on + strand at 859.923 kb on + strand at 859.923 kb on + strand at 859.923 kb on + strand at 859.925 kb on + strand at 859.925 kb on + strand at 859.925 kb on + strand at 859.925 kb on + strand at 859.925 kb on + strand at 859.925 kb on + strand at 859.925 kb on + strand at 859.925 kb on + strand at 859.926 kb on - strand at 859.926 kb on - strand at 859.926 kb on - strand at 859.926 kb on - strand at 859.959 kb on + strand at 859.960 kb on - strand at 859.961 kb on + strand at 859.962 kb on - strand at 859.963 kb on + strand at 859.963 kb on + strand at 859.963 kb on + strand at 859.963 kb on + strand at 859.963 kb on + strand at 859.964 kb on - strand at 859.964 kb on - strand at 859.964 kb on - strand at 859.964 kb on - strand at 860.053 kb on - strand at 860.053 kb on - strand at 860.100 kb on + strand at 860.101 kb on - strand at 860.101 kb on - strand at 860.101 kb on - strand at 860.101 kb on - strand at 860.180 kb on + strand, within Pf1N1B4_756 at 860.181 kb on - strand, within Pf1N1B4_756 at 860.181 kb on - strand, within Pf1N1B4_756 at 860.181 kb on - strand at 860.183 kb on - strand, within Pf1N1B4_756 at 860.185 kb on - strand, within Pf1N1B4_756 at 860.372 kb on + strand at 860.558 kb on - strand at 860.569 kb on - strand at 860.708 kb on + strand, within Pf1N1B4_757 at 860.709 kb on - strand, within Pf1N1B4_757 at 860.719 kb on + strand, within Pf1N1B4_757 at 860.720 kb on - strand, within Pf1N1B4_757 at 860.777 kb on + strand, within Pf1N1B4_757 at 860.784 kb on - strand, within Pf1N1B4_757 at 860.784 kb on - strand, within Pf1N1B4_757 at 860.835 kb on + strand, within Pf1N1B4_757 at 860.835 kb on + strand, within Pf1N1B4_757 at 860.835 kb on + strand, within Pf1N1B4_757 at 860.835 kb on + strand, within Pf1N1B4_757 at 860.835 kb on + strand, within Pf1N1B4_757 at 860.835 kb on + strand, within Pf1N1B4_757 at 860.835 kb on + strand, within Pf1N1B4_757 at 860.836 kb on - strand, within Pf1N1B4_757 at 860.836 kb on - strand at 860.836 kb on - strand, within Pf1N1B4_757 at 860.836 kb on - strand, within Pf1N1B4_757 at 860.976 kb on + strand at 860.976 kb on + strand, within Pf1N1B4_757 at 861.037 kb on - strand, within Pf1N1B4_757 at 861.037 kb on - strand, within Pf1N1B4_757 at 861.037 kb on - strand, within Pf1N1B4_757 at 861.037 kb on - strand, within Pf1N1B4_757 at 861.049 kb on + strand, within Pf1N1B4_757 at 861.049 kb on + strand, within Pf1N1B4_757 at 861.049 kb on + strand, within Pf1N1B4_757 at 861.050 kb on - strand, within Pf1N1B4_757 at 861.050 kb on - strand, within Pf1N1B4_757 at 861.050 kb on - strand, within Pf1N1B4_757 at 861.079 kb on - strand, within Pf1N1B4_757 at 861.188 kb on + strand, within Pf1N1B4_757 at 861.189 kb on - strand, within Pf1N1B4_757 at 861.284 kb on + strand, within Pf1N1B4_757 at 861.284 kb on + strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 859,237 + Pf1N1B4_755 0.54 +0.7 859,237 + Pf1N1B4_755 0.54 -0.3 859,392 + Pf1N1B4_755 0.64 -0.2 859,392 + Pf1N1B4_755 0.64 -0.2 859,392 + -0.3 859,392 + Pf1N1B4_755 0.64 -1.1 859,392 + Pf1N1B4_755 0.64 +1.5 859,392 + Pf1N1B4_755 0.64 +1.9 859,392 + Pf1N1B4_755 0.64 -2.2 859,393 - Pf1N1B4_755 0.64 +0.4 859,393 - Pf1N1B4_755 0.64 +1.1 859,393 - Pf1N1B4_755 0.64 +0.6 859,393 - Pf1N1B4_755 0.64 +0.7 859,393 - Pf1N1B4_755 0.64 -0.7 859,393 - Pf1N1B4_755 0.64 +0.2 859,396 + Pf1N1B4_755 0.64 +0.7 859,396 + Pf1N1B4_755 0.64 -2.6 859,396 + Pf1N1B4_755 0.64 -3.3 859,396 + Pf1N1B4_755 0.64 -0.5 859,397 - Pf1N1B4_755 0.64 -0.2 859,397 - Pf1N1B4_755 0.64 -3.0 859,397 - Pf1N1B4_755 0.64 -0.8 859,397 - Pf1N1B4_755 0.64 +0.4 859,450 + Pf1N1B4_755 0.68 -2.9 859,450 + Pf1N1B4_755 0.68 -0.0 859,450 + Pf1N1B4_755 0.68 -1.6 859,450 + Pf1N1B4_755 0.68 -0.4 859,451 - Pf1N1B4_755 0.68 +2.3 859,451 - Pf1N1B4_755 0.68 +2.1 859,451 - Pf1N1B4_755 0.68 -2.6 859,634 + Pf1N1B4_755 0.80 -2.1 859,634 + Pf1N1B4_755 0.80 +1.2 859,634 + Pf1N1B4_755 0.80 -0.5 859,635 - Pf1N1B4_755 0.80 -1.9 859,635 - Pf1N1B4_755 0.80 -0.4 859,635 - Pf1N1B4_755 0.80 +1.1 859,635 - Pf1N1B4_755 0.80 +1.1 859,635 - -2.3 859,635 - Pf1N1B4_755 0.80 -0.9 859,658 + Pf1N1B4_755 0.82 +1.6 859,658 + +0.1 859,658 + Pf1N1B4_755 0.82 -1.0 859,658 + Pf1N1B4_755 0.82 -0.4 859,658 + Pf1N1B4_755 0.82 +1.2 859,658 + Pf1N1B4_755 0.82 -0.0 859,658 + Pf1N1B4_755 0.82 -1.6 859,658 + Pf1N1B4_755 0.82 +0.7 859,658 + Pf1N1B4_755 0.82 +1.2 859,659 + -1.3 859,659 - Pf1N1B4_755 0.82 -0.2 859,659 - Pf1N1B4_755 0.82 +0.5 859,659 - Pf1N1B4_755 0.82 -0.6 859,659 - Pf1N1B4_755 0.82 -1.8 859,659 - Pf1N1B4_755 0.82 -2.8 859,659 - Pf1N1B4_755 0.82 -0.8 859,662 + Pf1N1B4_755 0.82 +0.9 859,673 + +1.1 859,673 + Pf1N1B4_755 0.83 -2.1 859,673 + Pf1N1B4_755 0.83 -2.4 859,673 + Pf1N1B4_755 0.83 -1.5 859,673 + Pf1N1B4_755 0.83 +2.3 859,674 - Pf1N1B4_755 0.83 +1.1 859,674 - Pf1N1B4_755 0.83 +0.7 859,674 - Pf1N1B4_755 0.83 +0.7 859,674 - Pf1N1B4_755 0.83 -1.9 859,674 - -1.5 859,674 - Pf1N1B4_755 0.83 -0.8 859,687 + Pf1N1B4_755 0.84 +1.2 859,739 + Pf1N1B4_755 0.88 +2.1 859,745 + Pf1N1B4_755 0.88 -2.4 859,745 + Pf1N1B4_755 0.88 +0.4 859,745 + Pf1N1B4_755 0.88 +0.1 859,745 + Pf1N1B4_755 0.88 +1.5 859,746 - Pf1N1B4_755 0.88 +0.1 859,746 - Pf1N1B4_755 0.88 -0.8 859,746 - Pf1N1B4_755 0.88 -1.6 859,814 + -0.9 859,815 - -1.1 859,815 - -0.3 859,903 + +1.4 859,903 + +0.7 859,904 - +2.0 859,904 - -0.9 859,923 + +0.5 859,923 + +1.1 859,923 + +1.4 859,923 + +1.3 859,923 + -3.4 859,923 + +0.9 859,923 + -1.8 859,923 + +1.9 859,925 + -0.5 859,925 + +2.6 859,925 + +1.7 859,925 + -1.4 859,925 + +1.5 859,925 + -0.5 859,925 + +2.3 859,925 + -0.5 859,926 - -2.1 859,926 - +0.7 859,926 - -1.8 859,926 - -1.4 859,959 + -1.1 859,960 - +0.1 859,961 + +0.7 859,962 - -0.2 859,963 + +0.8 859,963 + +0.1 859,963 + -3.0 859,963 + -0.1 859,963 + +0.2 859,964 - +1.9 859,964 - +1.4 859,964 - -1.0 859,964 - -0.0 860,053 - -0.0 860,053 - -0.5 860,100 + -1.7 860,101 - +0.5 860,101 - -0.2 860,101 - +0.0 860,101 - +0.8 860,180 + Pf1N1B4_756 0.43 +0.8 860,181 - Pf1N1B4_756 0.43 +1.9 860,181 - Pf1N1B4_756 0.43 -0.2 860,181 - -0.8 860,183 - Pf1N1B4_756 0.44 +0.5 860,185 - Pf1N1B4_756 0.46 -0.9 860,372 + -0.5 860,558 - -0.4 860,569 - -0.9 860,708 + Pf1N1B4_757 0.12 -1.3 860,709 - Pf1N1B4_757 0.12 +0.1 860,719 + Pf1N1B4_757 0.13 +0.1 860,720 - Pf1N1B4_757 0.13 +0.4 860,777 + Pf1N1B4_757 0.17 +1.4 860,784 - Pf1N1B4_757 0.18 -0.7 860,784 - Pf1N1B4_757 0.18 -2.3 860,835 + Pf1N1B4_757 0.22 -0.7 860,835 + Pf1N1B4_757 0.22 -1.9 860,835 + Pf1N1B4_757 0.22 -2.2 860,835 + Pf1N1B4_757 0.22 +1.2 860,835 + Pf1N1B4_757 0.22 -0.9 860,835 + Pf1N1B4_757 0.22 -1.8 860,835 + Pf1N1B4_757 0.22 +0.6 860,836 - Pf1N1B4_757 0.22 -3.0 860,836 - +1.9 860,836 - Pf1N1B4_757 0.22 +0.4 860,836 - Pf1N1B4_757 0.22 -0.9 860,976 + -1.4 860,976 + Pf1N1B4_757 0.33 -2.4 861,037 - Pf1N1B4_757 0.38 +0.6 861,037 - Pf1N1B4_757 0.38 -1.0 861,037 - Pf1N1B4_757 0.38 +0.7 861,037 - Pf1N1B4_757 0.38 +0.9 861,049 + Pf1N1B4_757 0.39 +1.5 861,049 + Pf1N1B4_757 0.39 -1.9 861,049 + Pf1N1B4_757 0.39 +0.7 861,050 - Pf1N1B4_757 0.39 +1.0 861,050 - Pf1N1B4_757 0.39 -1.6 861,050 - Pf1N1B4_757 0.39 +0.1 861,079 - Pf1N1B4_757 0.41 -0.9 861,188 + Pf1N1B4_757 0.50 -0.7 861,189 - Pf1N1B4_757 0.50 -1.3 861,284 + Pf1N1B4_757 0.58 +0.2 861,284 + Pf1N1B4_757 0.58 +1.1 861,285 - Pf1N1B4_757 0.58 +0.9 861,285 - Pf1N1B4_757 0.58 -0.1 861,285 - Pf1N1B4_757 0.58 -0.3 861,285 - Pf1N1B4_757 0.58 -0.2 861,285 - Pf1N1B4_757 0.58 +0.2 861,285 - Pf1N1B4_757 0.58 -1.1 861,285 - Pf1N1B4_757 0.58 -0.3
Or see this region's nucleotide sequence