Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6049 and Pf1N1B4_6050 overlap by 4 nucleotides Pf1N1B4_6050 and Pf1N1B4_6056 are separated by 277 nucleotides Pf1N1B4_6056 and Pf1N1B4_6057 are separated by 96 nucleotides Pf1N1B4_6057 and Pf1N1B4_6058 are separated by 58 nucleotides Pf1N1B4_6058 and Pf1N1B4_6051 are separated by 247 nucleotides
Pf1N1B4_6049 - Fe2+-dicitrate sensor, membrane component, at 516,786 to 517,730
_6049
Pf1N1B4_6050 - RNA polymerase sigma-70 factor, ECF subfamily, at 517,727 to 518,221
_6050
Pf1N1B4_6056 - tRNA-Asp-GTC, at 518,499 to 518,572
_6056
Pf1N1B4_6057 - tRNA-Asp-GTC, at 518,669 to 518,742
_6057
Pf1N1B4_6058 - tRNA-Val-TAC, at 518,801 to 518,873
_6058
Pf1N1B4_6051 - Oxidoreductase, short chain dehydrogenase/reductase family, at 519,121 to 519,552
_6051
Position (kb) 517
518
519 Strain fitness (log2 ratio) -2
-1
0
1
2 at 516.794 kb on + strand at 517.395 kb on - strand, within Pf1N1B4_6049 at 517.491 kb on + strand, within Pf1N1B4_6049 at 517.492 kb on - strand, within Pf1N1B4_6049 at 517.756 kb on - strand at 517.770 kb on + strand at 517.770 kb on + strand at 517.770 kb on + strand at 517.771 kb on - strand at 517.771 kb on - strand at 517.822 kb on - strand, within Pf1N1B4_6050 at 517.858 kb on + strand at 517.859 kb on - strand, within Pf1N1B4_6050 at 517.862 kb on + strand at 517.980 kb on + strand, within Pf1N1B4_6050 at 517.981 kb on - strand, within Pf1N1B4_6050 at 518.008 kb on - strand, within Pf1N1B4_6050 at 518.046 kb on + strand at 518.046 kb on + strand, within Pf1N1B4_6050 at 518.108 kb on - strand, within Pf1N1B4_6050 at 518.316 kb on - strand at 518.495 kb on - strand at 518.599 kb on - strand at 519.045 kb on - strand at 519.114 kb on - strand at 519.132 kb on - strand at 519.132 kb on - strand at 519.132 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 516,794 + -1.6 517,395 - Pf1N1B4_6049 0.64 -1.1 517,491 + Pf1N1B4_6049 0.75 -0.2 517,492 - Pf1N1B4_6049 0.75 +0.5 517,756 - -2.1 517,770 + -0.2 517,770 + -1.0 517,770 + +0.3 517,771 - +0.1 517,771 - +1.0 517,822 - Pf1N1B4_6050 0.19 +2.1 517,858 + -0.2 517,859 - Pf1N1B4_6050 0.27 -1.6 517,862 + -1.0 517,980 + Pf1N1B4_6050 0.51 +1.1 517,981 - Pf1N1B4_6050 0.51 +0.6 518,008 - Pf1N1B4_6050 0.57 +1.3 518,046 + -1.9 518,046 + Pf1N1B4_6050 0.64 +2.0 518,108 - Pf1N1B4_6050 0.77 +0.6 518,316 - -0.1 518,495 - -0.8 518,599 - +0.3 519,045 - +0.7 519,114 - +0.1 519,132 - +0.1 519,132 - -0.6 519,132 - +0.5
Or see this region's nucleotide sequence