Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5853 and Pf1N1B4_5854 overlap by 17 nucleotides Pf1N1B4_5854 and Pf1N1B4_5855 are separated by 57 nucleotides Pf1N1B4_5855 and Pf1N1B4_5856 are separated by 119 nucleotides
Pf1N1B4_5853 - Glutamine synthetase (EC 6.3.1.2), putative, at 285,255 to 286,586
_5853
Pf1N1B4_5854 - FIG00960144: hypothetical protein, at 286,570 to 287,322
_5854
Pf1N1B4_5855 - Putrescine importer, at 287,380 to 288,729
_5855
Pf1N1B4_5856 - Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109), at 288,849 to 289,880
_5856
Position (kb)
287
288
289 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 286.570 kb on - strand at 286.607 kb on - strand at 286.624 kb on - strand at 286.819 kb on + strand at 286.819 kb on + strand, within Pf1N1B4_5854 at 286.819 kb on + strand, within Pf1N1B4_5854 at 286.820 kb on - strand, within Pf1N1B4_5854 at 287.051 kb on + strand, within Pf1N1B4_5854 at 287.051 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.092 kb on + strand, within Pf1N1B4_5854 at 287.093 kb on - strand, within Pf1N1B4_5854 at 287.173 kb on + strand, within Pf1N1B4_5854 at 287.302 kb on + strand at 287.320 kb on + strand at 287.328 kb on + strand at 287.329 kb on - strand at 287.329 kb on - strand at 287.387 kb on + strand at 287.536 kb on + strand, within Pf1N1B4_5855 at 287.536 kb on + strand, within Pf1N1B4_5855 at 287.537 kb on - strand, within Pf1N1B4_5855 at 287.620 kb on + strand, within Pf1N1B4_5855 at 287.620 kb on + strand, within Pf1N1B4_5855 at 287.621 kb on - strand at 287.661 kb on + strand, within Pf1N1B4_5855 at 287.690 kb on - strand, within Pf1N1B4_5855 at 287.820 kb on + strand, within Pf1N1B4_5855 at 287.821 kb on - strand, within Pf1N1B4_5855 at 287.852 kb on + strand, within Pf1N1B4_5855 at 287.891 kb on - strand, within Pf1N1B4_5855 at 287.922 kb on + strand, within Pf1N1B4_5855 at 287.950 kb on + strand, within Pf1N1B4_5855 at 287.951 kb on - strand, within Pf1N1B4_5855 at 287.951 kb on - strand, within Pf1N1B4_5855 at 287.951 kb on - strand, within Pf1N1B4_5855 at 288.194 kb on - strand, within Pf1N1B4_5855 at 288.382 kb on + strand, within Pf1N1B4_5855 at 288.394 kb on + strand, within Pf1N1B4_5855 at 288.394 kb on + strand, within Pf1N1B4_5855 at 288.395 kb on - strand, within Pf1N1B4_5855 at 288.395 kb on - strand, within Pf1N1B4_5855 at 288.520 kb on + strand, within Pf1N1B4_5855 at 288.520 kb on + strand, within Pf1N1B4_5855 at 288.520 kb on + strand, within Pf1N1B4_5855 at 288.520 kb on + strand, within Pf1N1B4_5855 at 288.520 kb on + strand, within Pf1N1B4_5855 at 288.520 kb on + strand, within Pf1N1B4_5855 at 288.520 kb on + strand, within Pf1N1B4_5855 at 288.521 kb on - strand, within Pf1N1B4_5855 at 288.521 kb on - strand, within Pf1N1B4_5855 at 288.523 kb on + strand, within Pf1N1B4_5855 at 288.607 kb on + strand at 288.607 kb on + strand at 288.607 kb on + strand at 288.607 kb on + strand at 288.607 kb on + strand at 288.607 kb on + strand at 288.607 kb on + strand at 288.608 kb on - strand at 288.608 kb on - strand at 288.667 kb on + strand at 288.667 kb on + strand at 288.668 kb on - strand at 288.668 kb on - strand at 288.671 kb on + strand at 288.671 kb on + strand at 288.671 kb on + strand at 288.671 kb on + strand at 288.671 kb on + strand at 288.671 kb on + strand at 288.799 kb on + strand at 288.799 kb on + strand at 288.842 kb on + strand at 288.842 kb on + strand at 288.842 kb on + strand at 288.842 kb on + strand at 288.842 kb on + strand at 288.842 kb on + strand at 288.843 kb on - strand at 288.859 kb on + strand at 289.023 kb on + strand, within Pf1N1B4_5856 at 289.023 kb on + strand, within Pf1N1B4_5856 at 289.024 kb on - strand, within Pf1N1B4_5856 at 289.024 kb on - strand, within Pf1N1B4_5856 at 289.209 kb on - strand, within Pf1N1B4_5856 at 289.209 kb on - strand, within Pf1N1B4_5856 at 289.267 kb on + strand, within Pf1N1B4_5856 at 289.267 kb on + strand, within Pf1N1B4_5856 at 289.267 kb on + strand, within Pf1N1B4_5856 at 289.347 kb on + strand, within Pf1N1B4_5856 at 289.413 kb on + strand at 289.596 kb on + strand, within Pf1N1B4_5856 at 289.596 kb on + strand, within Pf1N1B4_5856 at 289.596 kb on + strand, within Pf1N1B4_5856 at 289.597 kb on - strand, within Pf1N1B4_5856 at 289.597 kb on - strand, within Pf1N1B4_5856 at 289.597 kb on - strand, within Pf1N1B4_5856 at 289.597 kb on - strand, within Pf1N1B4_5856 at 289.597 kb on - strand, within Pf1N1B4_5856 at 289.600 kb on + strand, within Pf1N1B4_5856 at 289.601 kb on - strand, within Pf1N1B4_5856 at 289.601 kb on - strand, within Pf1N1B4_5856
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 286,570 - +0.5 286,607 - -1.4 286,624 - -1.5 286,819 + -1.2 286,819 + Pf1N1B4_5854 0.33 -1.7 286,819 + Pf1N1B4_5854 0.33 -0.3 286,820 - Pf1N1B4_5854 0.33 +1.2 287,051 + Pf1N1B4_5854 0.64 -1.0 287,051 + Pf1N1B4_5854 0.64 -0.7 287,053 + Pf1N1B4_5854 0.64 -1.2 287,053 + Pf1N1B4_5854 0.64 +1.9 287,053 + Pf1N1B4_5854 0.64 +2.2 287,053 + Pf1N1B4_5854 0.64 +0.5 287,053 + Pf1N1B4_5854 0.64 +0.3 287,092 + Pf1N1B4_5854 0.69 +0.7 287,093 - Pf1N1B4_5854 0.69 +0.8 287,173 + Pf1N1B4_5854 0.80 +0.6 287,302 + +0.6 287,320 + +1.0 287,328 + -1.1 287,329 - +1.3 287,329 - +0.9 287,387 + +1.7 287,536 + Pf1N1B4_5855 0.12 +1.3 287,536 + Pf1N1B4_5855 0.12 +1.7 287,537 - Pf1N1B4_5855 0.12 -3.0 287,620 + Pf1N1B4_5855 0.18 -1.7 287,620 + Pf1N1B4_5855 0.18 +0.7 287,621 - -0.7 287,661 + Pf1N1B4_5855 0.21 +0.2 287,690 - Pf1N1B4_5855 0.23 -1.2 287,820 + Pf1N1B4_5855 0.33 +1.3 287,821 - Pf1N1B4_5855 0.33 +2.4 287,852 + Pf1N1B4_5855 0.35 -2.1 287,891 - Pf1N1B4_5855 0.38 -1.9 287,922 + Pf1N1B4_5855 0.40 -0.4 287,950 + Pf1N1B4_5855 0.42 +1.0 287,951 - Pf1N1B4_5855 0.42 +0.4 287,951 - Pf1N1B4_5855 0.42 +0.5 287,951 - Pf1N1B4_5855 0.42 +1.3 288,194 - Pf1N1B4_5855 0.60 -0.2 288,382 + Pf1N1B4_5855 0.74 -0.4 288,394 + Pf1N1B4_5855 0.75 +1.5 288,394 + Pf1N1B4_5855 0.75 +1.7 288,395 - Pf1N1B4_5855 0.75 +1.6 288,395 - Pf1N1B4_5855 0.75 +1.8 288,520 + Pf1N1B4_5855 0.84 +0.6 288,520 + Pf1N1B4_5855 0.84 +1.4 288,520 + Pf1N1B4_5855 0.84 +0.2 288,520 + Pf1N1B4_5855 0.84 -3.0 288,520 + Pf1N1B4_5855 0.84 +0.4 288,520 + Pf1N1B4_5855 0.84 +0.6 288,520 + Pf1N1B4_5855 0.84 +0.3 288,521 - Pf1N1B4_5855 0.85 +0.4 288,521 - Pf1N1B4_5855 0.85 +1.1 288,523 + Pf1N1B4_5855 0.85 -0.1 288,607 + +1.7 288,607 + +1.1 288,607 + -1.9 288,607 + +1.3 288,607 + +1.2 288,607 + +0.0 288,607 + +1.7 288,608 - +2.3 288,608 - +0.2 288,667 + +1.4 288,667 + +0.6 288,668 - +1.8 288,668 - -0.1 288,671 + -0.7 288,671 + +0.4 288,671 + -1.2 288,671 + -1.3 288,671 + +0.3 288,671 + -2.9 288,799 + +0.3 288,799 + +2.3 288,842 + +1.5 288,842 + +0.1 288,842 + -1.5 288,842 + +2.1 288,842 + -1.7 288,842 + +0.8 288,843 - +0.7 288,859 + -0.6 289,023 + Pf1N1B4_5856 0.17 +0.8 289,023 + Pf1N1B4_5856 0.17 +1.5 289,024 - Pf1N1B4_5856 0.17 -0.3 289,024 - Pf1N1B4_5856 0.17 -0.2 289,209 - Pf1N1B4_5856 0.35 +1.8 289,209 - Pf1N1B4_5856 0.35 +2.3 289,267 + Pf1N1B4_5856 0.41 -2.2 289,267 + Pf1N1B4_5856 0.41 -0.7 289,267 + Pf1N1B4_5856 0.41 -2.4 289,347 + Pf1N1B4_5856 0.48 -0.4 289,413 + -0.2 289,596 + Pf1N1B4_5856 0.72 +1.3 289,596 + Pf1N1B4_5856 0.72 -3.0 289,596 + Pf1N1B4_5856 0.72 +0.3 289,597 - Pf1N1B4_5856 0.72 -0.3 289,597 - Pf1N1B4_5856 0.72 +0.8 289,597 - Pf1N1B4_5856 0.72 +0.6 289,597 - Pf1N1B4_5856 0.72 +0.2 289,597 - Pf1N1B4_5856 0.72 +0.8 289,600 + Pf1N1B4_5856 0.73 +0.3 289,601 - Pf1N1B4_5856 0.73 +1.6 289,601 - Pf1N1B4_5856 0.73 +1.1
Or see this region's nucleotide sequence