Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5800 and Pf1N1B4_5801 overlap by 27 nucleotides Pf1N1B4_5801 and Pf1N1B4_5802 are separated by 146 nucleotides
Pf1N1B4_5800 - Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10), at 235,274 to 236,638
_5800
Pf1N1B4_5801 - hypothetical protein, at 236,612 to 236,746
_5801
Pf1N1B4_5802 - benABC operon transcriptional activator BenR, at 236,893 to 237,744
_5802
Position (kb)
236
237 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 235.632 kb on + strand, within Pf1N1B4_5800 at 235.633 kb on - strand, within Pf1N1B4_5800 at 235.633 kb on - strand, within Pf1N1B4_5800 at 235.707 kb on + strand, within Pf1N1B4_5800 at 235.803 kb on + strand, within Pf1N1B4_5800 at 235.870 kb on - strand, within Pf1N1B4_5800 at 235.870 kb on - strand, within Pf1N1B4_5800 at 236.037 kb on + strand, within Pf1N1B4_5800 at 236.037 kb on + strand, within Pf1N1B4_5800 at 236.037 kb on + strand, within Pf1N1B4_5800 at 236.038 kb on - strand, within Pf1N1B4_5800 at 236.043 kb on + strand, within Pf1N1B4_5800 at 236.043 kb on + strand, within Pf1N1B4_5800 at 236.043 kb on + strand, within Pf1N1B4_5800 at 236.043 kb on + strand, within Pf1N1B4_5800 at 236.043 kb on + strand, within Pf1N1B4_5800 at 236.043 kb on + strand, within Pf1N1B4_5800 at 236.043 kb on + strand, within Pf1N1B4_5800 at 236.044 kb on - strand, within Pf1N1B4_5800 at 236.044 kb on - strand, within Pf1N1B4_5800 at 236.322 kb on + strand, within Pf1N1B4_5800 at 236.322 kb on + strand, within Pf1N1B4_5800 at 236.323 kb on - strand, within Pf1N1B4_5800 at 236.323 kb on - strand, within Pf1N1B4_5800 at 236.323 kb on - strand, within Pf1N1B4_5800 at 236.323 kb on - strand, within Pf1N1B4_5800 at 236.323 kb on - strand, within Pf1N1B4_5800 at 236.323 kb on - strand at 236.386 kb on + strand, within Pf1N1B4_5800 at 236.454 kb on + strand, within Pf1N1B4_5800 at 236.497 kb on - strand, within Pf1N1B4_5800 at 236.577 kb on + strand at 236.577 kb on + strand at 236.619 kb on + strand at 236.723 kb on + strand, within Pf1N1B4_5801 at 237.046 kb on - strand, within Pf1N1B4_5802 at 237.046 kb on - strand, within Pf1N1B4_5802 at 237.046 kb on - strand, within Pf1N1B4_5802 at 237.072 kb on - strand, within Pf1N1B4_5802 at 237.113 kb on + strand, within Pf1N1B4_5802 at 237.192 kb on - strand, within Pf1N1B4_5802 at 237.192 kb on - strand, within Pf1N1B4_5802 at 237.294 kb on - strand at 237.507 kb on - strand, within Pf1N1B4_5802 at 237.507 kb on - strand, within Pf1N1B4_5802 at 237.507 kb on - strand, within Pf1N1B4_5802 at 237.507 kb on - strand, within Pf1N1B4_5802 at 237.564 kb on - strand, within Pf1N1B4_5802 at 237.564 kb on - strand, within Pf1N1B4_5802 at 237.564 kb on - strand, within Pf1N1B4_5802 at 237.564 kb on - strand, within Pf1N1B4_5802 at 237.623 kb on + strand, within Pf1N1B4_5802 at 237.624 kb on - strand, within Pf1N1B4_5802 at 237.648 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 235,632 + Pf1N1B4_5800 0.26 +0.7 235,633 - Pf1N1B4_5800 0.26 +1.2 235,633 - Pf1N1B4_5800 0.26 -0.2 235,707 + Pf1N1B4_5800 0.32 -0.7 235,803 + Pf1N1B4_5800 0.39 -1.7 235,870 - Pf1N1B4_5800 0.44 +0.0 235,870 - Pf1N1B4_5800 0.44 -0.2 236,037 + Pf1N1B4_5800 0.56 -0.3 236,037 + Pf1N1B4_5800 0.56 +0.2 236,037 + Pf1N1B4_5800 0.56 -0.9 236,038 - Pf1N1B4_5800 0.56 -2.4 236,043 + Pf1N1B4_5800 0.56 +1.0 236,043 + Pf1N1B4_5800 0.56 -0.3 236,043 + Pf1N1B4_5800 0.56 -0.1 236,043 + Pf1N1B4_5800 0.56 +0.7 236,043 + Pf1N1B4_5800 0.56 -1.5 236,043 + Pf1N1B4_5800 0.56 +0.4 236,043 + Pf1N1B4_5800 0.56 +2.0 236,044 - Pf1N1B4_5800 0.56 +0.3 236,044 - Pf1N1B4_5800 0.56 +0.9 236,322 + Pf1N1B4_5800 0.77 +0.7 236,322 + Pf1N1B4_5800 0.77 +2.8 236,323 - Pf1N1B4_5800 0.77 +0.4 236,323 - Pf1N1B4_5800 0.77 -0.1 236,323 - Pf1N1B4_5800 0.77 +1.6 236,323 - Pf1N1B4_5800 0.77 +0.8 236,323 - Pf1N1B4_5800 0.77 +0.5 236,323 - -0.5 236,386 + Pf1N1B4_5800 0.81 +0.2 236,454 + Pf1N1B4_5800 0.86 +0.3 236,497 - Pf1N1B4_5800 0.90 +0.2 236,577 + -0.7 236,577 + +0.8 236,619 + +0.5 236,723 + Pf1N1B4_5801 0.82 +0.7 237,046 - Pf1N1B4_5802 0.18 +0.2 237,046 - Pf1N1B4_5802 0.18 +1.3 237,046 - Pf1N1B4_5802 0.18 -2.1 237,072 - Pf1N1B4_5802 0.21 -2.5 237,113 + Pf1N1B4_5802 0.26 -1.2 237,192 - Pf1N1B4_5802 0.35 +0.2 237,192 - Pf1N1B4_5802 0.35 -0.7 237,294 - -0.2 237,507 - Pf1N1B4_5802 0.72 +1.0 237,507 - Pf1N1B4_5802 0.72 +0.7 237,507 - Pf1N1B4_5802 0.72 +1.8 237,507 - Pf1N1B4_5802 0.72 -3.2 237,564 - Pf1N1B4_5802 0.79 +2.8 237,564 - Pf1N1B4_5802 0.79 -0.2 237,564 - Pf1N1B4_5802 0.79 +0.4 237,564 - Pf1N1B4_5802 0.79 -0.1 237,623 + Pf1N1B4_5802 0.86 +0.6 237,624 - Pf1N1B4_5802 0.86 -0.3 237,648 + +1.0
Or see this region's nucleotide sequence