Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5700 and Pf1N1B4_5701 are separated by 169 nucleotides Pf1N1B4_5701 and Pf1N1B4_5702 overlap by 14 nucleotides Pf1N1B4_5702 and Pf1N1B4_5703 are separated by 42 nucleotides Pf1N1B4_5703 and Pf1N1B4_5704 are separated by 82 nucleotides
Pf1N1B4_5700 - Putative tRNA-m1A22 methylase, at 139,167 to 139,841
_5700
Pf1N1B4_5701 - FIG00955602: hypothetical protein, at 140,011 to 140,172
_5701
Pf1N1B4_5702 - hypothetical protein, at 140,159 to 140,320
_5702
Pf1N1B4_5703 - FIG00961921: hypothetical protein, at 140,363 to 141,073
_5703
Pf1N1B4_5704 - Long-chain fatty acid transport protein, at 141,156 to 142,475
_5704
Position (kb) 140
141
142 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 139.363 kb on + strand, within Pf1N1B4_5700 at 139.363 kb on + strand, within Pf1N1B4_5700 at 139.363 kb on + strand, within Pf1N1B4_5700 at 139.364 kb on - strand, within Pf1N1B4_5700 at 139.364 kb on - strand, within Pf1N1B4_5700 at 139.364 kb on - strand, within Pf1N1B4_5700 at 139.403 kb on - strand, within Pf1N1B4_5700 at 139.406 kb on - strand, within Pf1N1B4_5700 at 139.679 kb on + strand, within Pf1N1B4_5700 at 139.679 kb on + strand, within Pf1N1B4_5700 at 139.812 kb on + strand at 139.812 kb on + strand at 139.812 kb on + strand at 139.813 kb on - strand at 139.813 kb on - strand at 139.876 kb on + strand at 139.877 kb on - strand at 140.028 kb on + strand, within Pf1N1B4_5701 at 140.076 kb on + strand, within Pf1N1B4_5701 at 140.077 kb on - strand, within Pf1N1B4_5701 at 140.077 kb on - strand at 140.077 kb on - strand, within Pf1N1B4_5701 at 140.085 kb on - strand at 140.088 kb on + strand, within Pf1N1B4_5701 at 140.089 kb on - strand, within Pf1N1B4_5701 at 140.095 kb on - strand, within Pf1N1B4_5701 at 140.095 kb on - strand, within Pf1N1B4_5701 at 140.141 kb on - strand, within Pf1N1B4_5701 at 140.143 kb on + strand, within Pf1N1B4_5701 at 140.143 kb on + strand, within Pf1N1B4_5701 at 140.172 kb on + strand at 140.172 kb on + strand at 140.328 kb on - strand at 140.339 kb on + strand at 140.654 kb on + strand, within Pf1N1B4_5703 at 140.654 kb on + strand, within Pf1N1B4_5703 at 140.654 kb on + strand, within Pf1N1B4_5703 at 140.654 kb on + strand, within Pf1N1B4_5703 at 140.655 kb on - strand, within Pf1N1B4_5703 at 140.696 kb on + strand, within Pf1N1B4_5703 at 140.835 kb on - strand, within Pf1N1B4_5703 at 141.049 kb on - strand at 141.218 kb on - strand at 141.218 kb on - strand at 141.660 kb on - strand, within Pf1N1B4_5704 at 141.660 kb on - strand, within Pf1N1B4_5704 at 141.666 kb on + strand, within Pf1N1B4_5704 at 141.667 kb on - strand, within Pf1N1B4_5704 at 141.667 kb on - strand, within Pf1N1B4_5704 at 141.679 kb on + strand, within Pf1N1B4_5704 at 141.680 kb on - strand, within Pf1N1B4_5704 at 141.680 kb on - strand, within Pf1N1B4_5704 at 141.743 kb on - strand, within Pf1N1B4_5704 at 141.761 kb on - strand, within Pf1N1B4_5704 at 141.761 kb on - strand, within Pf1N1B4_5704 at 141.890 kb on - strand, within Pf1N1B4_5704 at 141.946 kb on + strand, within Pf1N1B4_5704 at 142.004 kb on - strand, within Pf1N1B4_5704 at 142.039 kb on + strand, within Pf1N1B4_5704 at 142.040 kb on - strand, within Pf1N1B4_5704 at 142.040 kb on - strand, within Pf1N1B4_5704 at 142.066 kb on + strand, within Pf1N1B4_5704
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 139,363 + Pf1N1B4_5700 0.29 +1.1 139,363 + Pf1N1B4_5700 0.29 +2.1 139,363 + Pf1N1B4_5700 0.29 +0.0 139,364 - Pf1N1B4_5700 0.29 +1.7 139,364 - Pf1N1B4_5700 0.29 -0.5 139,364 - Pf1N1B4_5700 0.29 +1.2 139,403 - Pf1N1B4_5700 0.35 +0.1 139,406 - Pf1N1B4_5700 0.35 -0.1 139,679 + Pf1N1B4_5700 0.76 +2.1 139,679 + Pf1N1B4_5700 0.76 -2.5 139,812 + -2.6 139,812 + -0.5 139,812 + -3.8 139,813 - -0.0 139,813 - +1.6 139,876 + -0.4 139,877 - -0.0 140,028 + Pf1N1B4_5701 0.10 -1.8 140,076 + Pf1N1B4_5701 0.40 +1.3 140,077 - Pf1N1B4_5701 0.41 +0.0 140,077 - -1.5 140,077 - Pf1N1B4_5701 0.41 +0.3 140,085 - +0.1 140,088 + Pf1N1B4_5701 0.48 +0.2 140,089 - Pf1N1B4_5701 0.48 +0.4 140,095 - Pf1N1B4_5701 0.52 -0.1 140,095 - Pf1N1B4_5701 0.52 +1.2 140,141 - Pf1N1B4_5701 0.80 -0.4 140,143 + Pf1N1B4_5701 0.81 +1.7 140,143 + Pf1N1B4_5701 0.81 -0.2 140,172 + -1.4 140,172 + +1.1 140,328 - +0.5 140,339 + -1.0 140,654 + Pf1N1B4_5703 0.41 +1.3 140,654 + Pf1N1B4_5703 0.41 +0.4 140,654 + Pf1N1B4_5703 0.41 +1.4 140,654 + Pf1N1B4_5703 0.41 +0.8 140,655 - Pf1N1B4_5703 0.41 -0.1 140,696 + Pf1N1B4_5703 0.47 +2.6 140,835 - Pf1N1B4_5703 0.66 +0.8 141,049 - -1.3 141,218 - -0.5 141,218 - +1.0 141,660 - Pf1N1B4_5704 0.38 -1.0 141,660 - Pf1N1B4_5704 0.38 -0.5 141,666 + Pf1N1B4_5704 0.39 +1.4 141,667 - Pf1N1B4_5704 0.39 +1.5 141,667 - Pf1N1B4_5704 0.39 +0.5 141,679 + Pf1N1B4_5704 0.40 +0.6 141,680 - Pf1N1B4_5704 0.40 +2.8 141,680 - Pf1N1B4_5704 0.40 -0.9 141,743 - Pf1N1B4_5704 0.44 -0.4 141,761 - Pf1N1B4_5704 0.46 +1.6 141,761 - Pf1N1B4_5704 0.46 +0.6 141,890 - Pf1N1B4_5704 0.56 -0.9 141,946 + Pf1N1B4_5704 0.60 -0.2 142,004 - Pf1N1B4_5704 0.64 +0.5 142,039 + Pf1N1B4_5704 0.67 -1.3 142,040 - Pf1N1B4_5704 0.67 -0.0 142,040 - Pf1N1B4_5704 0.67 +2.9 142,066 + Pf1N1B4_5704 0.69 -0.8
Or see this region's nucleotide sequence