Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5691 and Pf1N1B4_5692 are separated by 126 nucleotides Pf1N1B4_5692 and Pf1N1B4_5693 are separated by 17 nucleotides
Pf1N1B4_5691 - Chromosome initiation inhibitor, at 129,059 to 130,000
_5691
Pf1N1B4_5692 - Nitrate/nitrite transporter, at 130,127 to 131,422
_5692
Pf1N1B4_5693 - Gluconate dehydratase (EC 4.2.1.39), at 131,440 to 132,624
_5693
Position (kb) 130
131
132 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 129.479 kb on + strand, within Pf1N1B4_5691 at 129.480 kb on - strand, within Pf1N1B4_5691 at 129.493 kb on - strand, within Pf1N1B4_5691 at 129.955 kb on - strand at 130.080 kb on + strand at 130.110 kb on - strand at 130.197 kb on - strand at 130.445 kb on + strand at 130.446 kb on - strand, within Pf1N1B4_5692 at 130.446 kb on - strand, within Pf1N1B4_5692 at 130.559 kb on + strand, within Pf1N1B4_5692 at 130.703 kb on + strand, within Pf1N1B4_5692 at 130.853 kb on + strand, within Pf1N1B4_5692 at 130.853 kb on + strand, within Pf1N1B4_5692 at 130.854 kb on - strand, within Pf1N1B4_5692 at 130.854 kb on - strand, within Pf1N1B4_5692 at 130.892 kb on + strand, within Pf1N1B4_5692 at 130.907 kb on + strand, within Pf1N1B4_5692 at 130.907 kb on + strand, within Pf1N1B4_5692 at 131.501 kb on - strand at 131.560 kb on + strand, within Pf1N1B4_5693 at 131.687 kb on - strand, within Pf1N1B4_5693 at 131.687 kb on - strand, within Pf1N1B4_5693 at 131.764 kb on + strand, within Pf1N1B4_5693 at 131.779 kb on + strand, within Pf1N1B4_5693 at 131.779 kb on + strand, within Pf1N1B4_5693 at 131.780 kb on - strand, within Pf1N1B4_5693 at 131.914 kb on + strand at 131.914 kb on + strand, within Pf1N1B4_5693 at 132.106 kb on + strand, within Pf1N1B4_5693 at 132.107 kb on - strand, within Pf1N1B4_5693 at 132.107 kb on - strand, within Pf1N1B4_5693 at 132.212 kb on + strand, within Pf1N1B4_5693 at 132.213 kb on - strand at 132.406 kb on + strand, within Pf1N1B4_5693 at 132.413 kb on - strand, within Pf1N1B4_5693
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 129,479 + Pf1N1B4_5691 0.45 +1.5 129,480 - Pf1N1B4_5691 0.45 +1.1 129,493 - Pf1N1B4_5691 0.46 -0.2 129,955 - +1.2 130,080 + -0.1 130,110 - +0.1 130,197 - -0.6 130,445 + -1.5 130,446 - Pf1N1B4_5692 0.25 -1.3 130,446 - Pf1N1B4_5692 0.25 +0.1 130,559 + Pf1N1B4_5692 0.33 +1.8 130,703 + Pf1N1B4_5692 0.44 +0.7 130,853 + Pf1N1B4_5692 0.56 +0.6 130,853 + Pf1N1B4_5692 0.56 +2.8 130,854 - Pf1N1B4_5692 0.56 -0.2 130,854 - Pf1N1B4_5692 0.56 +1.2 130,892 + Pf1N1B4_5692 0.59 +0.5 130,907 + Pf1N1B4_5692 0.60 +0.5 130,907 + Pf1N1B4_5692 0.60 -0.0 131,501 - -1.3 131,560 + Pf1N1B4_5693 0.10 +0.9 131,687 - Pf1N1B4_5693 0.21 -2.7 131,687 - Pf1N1B4_5693 0.21 +0.8 131,764 + Pf1N1B4_5693 0.27 +0.4 131,779 + Pf1N1B4_5693 0.29 +1.1 131,779 + Pf1N1B4_5693 0.29 -0.3 131,780 - Pf1N1B4_5693 0.29 +0.6 131,914 + -1.6 131,914 + Pf1N1B4_5693 0.40 +1.2 132,106 + Pf1N1B4_5693 0.56 +3.2 132,107 - Pf1N1B4_5693 0.56 -0.5 132,107 - Pf1N1B4_5693 0.56 -0.6 132,212 + Pf1N1B4_5693 0.65 +0.1 132,213 - -2.5 132,406 + Pf1N1B4_5693 0.82 -1.9 132,413 - Pf1N1B4_5693 0.82 -0.2
Or see this region's nucleotide sequence