Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5551 and Pf1N1B4_5552 are separated by 182 nucleotides Pf1N1B4_5552 and Pf1N1B4_5553 are separated by 394 nucleotides
Pf1N1B4_5551 - hypothetical protein, at 323,086 to 324,072
_5551
Pf1N1B4_5552 - hypothetical protein, at 324,255 to 324,395
_5552
Pf1N1B4_5553 - Exodeoxyribonuclease I (EC 3.1.11.1), at 324,790 to 326,202
_5553
Position (kb) 324
325 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 323.464 kb on - strand, within Pf1N1B4_5551 at 323.464 kb on - strand, within Pf1N1B4_5551 at 323.498 kb on + strand, within Pf1N1B4_5551 at 323.499 kb on - strand, within Pf1N1B4_5551 at 323.564 kb on - strand, within Pf1N1B4_5551 at 323.576 kb on + strand, within Pf1N1B4_5551 at 323.576 kb on + strand, within Pf1N1B4_5551 at 323.576 kb on + strand at 323.577 kb on - strand, within Pf1N1B4_5551 at 323.577 kb on - strand, within Pf1N1B4_5551 at 323.577 kb on - strand, within Pf1N1B4_5551 at 323.791 kb on + strand, within Pf1N1B4_5551 at 323.791 kb on + strand, within Pf1N1B4_5551 at 323.791 kb on + strand, within Pf1N1B4_5551 at 323.792 kb on - strand, within Pf1N1B4_5551 at 323.935 kb on + strand, within Pf1N1B4_5551 at 324.012 kb on + strand at 324.059 kb on + strand at 324.092 kb on + strand at 324.210 kb on - strand at 324.210 kb on - strand at 324.251 kb on + strand at 324.252 kb on - strand at 324.262 kb on + strand at 324.262 kb on + strand at 324.263 kb on - strand at 324.306 kb on + strand, within Pf1N1B4_5552 at 324.307 kb on - strand, within Pf1N1B4_5552 at 324.307 kb on - strand, within Pf1N1B4_5552 at 324.307 kb on - strand, within Pf1N1B4_5552 at 324.335 kb on + strand, within Pf1N1B4_5552 at 324.360 kb on - strand, within Pf1N1B4_5552 at 324.360 kb on - strand, within Pf1N1B4_5552 at 324.582 kb on + strand at 324.582 kb on + strand at 324.583 kb on - strand at 324.604 kb on - strand at 324.699 kb on + strand at 324.700 kb on - strand at 324.721 kb on + strand at 324.722 kb on - strand at 324.722 kb on - strand at 324.882 kb on + strand at 324.882 kb on + strand at 324.883 kb on - strand at 324.883 kb on - strand at 324.883 kb on - strand at 324.883 kb on - strand at 324.926 kb on - strand at 325.004 kb on + strand at 325.005 kb on - strand, within Pf1N1B4_5553 at 325.130 kb on + strand, within Pf1N1B4_5553 at 325.130 kb on + strand, within Pf1N1B4_5553 at 325.135 kb on - strand at 325.240 kb on + strand, within Pf1N1B4_5553 at 325.240 kb on + strand at 325.241 kb on - strand, within Pf1N1B4_5553 at 325.241 kb on - strand, within Pf1N1B4_5553 at 325.241 kb on - strand, within Pf1N1B4_5553 at 325.241 kb on - strand, within Pf1N1B4_5553 at 325.241 kb on - strand, within Pf1N1B4_5553
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 323,464 - Pf1N1B4_5551 0.38 -0.2 323,464 - Pf1N1B4_5551 0.38 +2.2 323,498 + Pf1N1B4_5551 0.42 -0.7 323,499 - Pf1N1B4_5551 0.42 -1.3 323,564 - Pf1N1B4_5551 0.48 -0.4 323,576 + Pf1N1B4_5551 0.50 -1.7 323,576 + Pf1N1B4_5551 0.50 -1.8 323,576 + +1.1 323,577 - Pf1N1B4_5551 0.50 -0.8 323,577 - Pf1N1B4_5551 0.50 -0.4 323,577 - Pf1N1B4_5551 0.50 -1.5 323,791 + Pf1N1B4_5551 0.71 -2.4 323,791 + Pf1N1B4_5551 0.71 +1.9 323,791 + Pf1N1B4_5551 0.71 -0.4 323,792 - Pf1N1B4_5551 0.72 +1.2 323,935 + Pf1N1B4_5551 0.86 -0.1 324,012 + -2.9 324,059 + -1.4 324,092 + -0.3 324,210 - +0.6 324,210 - +0.8 324,251 + -1.2 324,252 - -0.2 324,262 + -0.3 324,262 + -0.4 324,263 - +0.8 324,306 + Pf1N1B4_5552 0.36 -3.0 324,307 - Pf1N1B4_5552 0.37 -2.5 324,307 - Pf1N1B4_5552 0.37 +0.7 324,307 - Pf1N1B4_5552 0.37 +2.8 324,335 + Pf1N1B4_5552 0.57 +0.6 324,360 - Pf1N1B4_5552 0.74 +0.6 324,360 - Pf1N1B4_5552 0.74 +1.9 324,582 + +1.4 324,582 + -3.0 324,583 - -1.0 324,604 - -0.4 324,699 + -0.4 324,700 - +1.3 324,721 + -1.7 324,722 - +1.3 324,722 - +1.6 324,882 + +1.4 324,882 + +0.9 324,883 - -0.7 324,883 - +0.2 324,883 - -2.3 324,883 - -0.6 324,926 - +0.8 325,004 + +0.4 325,005 - Pf1N1B4_5553 0.15 +1.2 325,130 + Pf1N1B4_5553 0.24 -0.8 325,130 + Pf1N1B4_5553 0.24 -0.4 325,135 - +0.9 325,240 + Pf1N1B4_5553 0.32 +1.0 325,240 + +1.4 325,241 - Pf1N1B4_5553 0.32 -0.9 325,241 - Pf1N1B4_5553 0.32 +1.2 325,241 - Pf1N1B4_5553 0.32 +0.5 325,241 - Pf1N1B4_5553 0.32 -2.0 325,241 - Pf1N1B4_5553 0.32 +0.7
Or see this region's nucleotide sequence