Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5019 and Pf1N1B4_5020 are separated by 212 nucleotides Pf1N1B4_5020 and Pf1N1B4_5021 are separated by 22 nucleotides Pf1N1B4_5021 and Pf1N1B4_5022 are separated by 127 nucleotides Pf1N1B4_5022 and Pf1N1B4_5023 overlap by 11 nucleotides Pf1N1B4_5023 and Pf1N1B4_5024 are separated by 60 nucleotides
Pf1N1B4_5019 - FIG00958773: hypothetical protein, at 5,568,806 to 5,569,162
_5019
Pf1N1B4_5020 - FIG00954920: hypothetical protein, at 5,569,375 to 5,569,677
_5020
Pf1N1B4_5021 - FIG00958580: hypothetical protein, at 5,569,700 to 5,569,876
_5021
Pf1N1B4_5022 - hypothetical protein, at 5,570,004 to 5,570,714
_5022
Pf1N1B4_5023 - Alkylated DNA repair protein AlkB, at 5,570,704 to 5,571,384
_5023
Pf1N1B4_5024 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 5,571,445 to 5,572,545
_5024
Position (kb) 5570
5571 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5569.064 kb on + strand, within Pf1N1B4_5019 at 5569.229 kb on - strand at 5569.237 kb on + strand at 5569.237 kb on + strand at 5569.237 kb on + strand at 5569.250 kb on - strand at 5569.699 kb on + strand at 5569.699 kb on + strand at 5569.700 kb on - strand at 5569.700 kb on - strand at 5569.725 kb on - strand at 5569.725 kb on - strand, within Pf1N1B4_5021 at 5570.009 kb on + strand at 5570.122 kb on + strand at 5570.122 kb on + strand, within Pf1N1B4_5022 at 5570.134 kb on - strand, within Pf1N1B4_5022 at 5570.199 kb on + strand, within Pf1N1B4_5022 at 5570.199 kb on + strand, within Pf1N1B4_5022 at 5570.200 kb on - strand, within Pf1N1B4_5022 at 5570.200 kb on - strand, within Pf1N1B4_5022 at 5570.206 kb on - strand at 5570.206 kb on - strand, within Pf1N1B4_5022 at 5570.289 kb on + strand, within Pf1N1B4_5022 at 5570.289 kb on + strand at 5570.394 kb on + strand, within Pf1N1B4_5022 at 5570.409 kb on + strand, within Pf1N1B4_5022 at 5570.410 kb on - strand, within Pf1N1B4_5022 at 5570.800 kb on + strand, within Pf1N1B4_5023 at 5570.801 kb on - strand, within Pf1N1B4_5023 at 5570.801 kb on - strand, within Pf1N1B4_5023 at 5570.801 kb on - strand, within Pf1N1B4_5023 at 5570.907 kb on + strand, within Pf1N1B4_5023 at 5570.908 kb on - strand at 5570.908 kb on - strand, within Pf1N1B4_5023 at 5570.974 kb on + strand, within Pf1N1B4_5023 at 5571.108 kb on + strand, within Pf1N1B4_5023 at 5571.109 kb on - strand, within Pf1N1B4_5023 at 5571.109 kb on - strand at 5571.109 kb on - strand, within Pf1N1B4_5023 at 5571.109 kb on - strand, within Pf1N1B4_5023 at 5571.418 kb on - strand at 5571.418 kb on - strand at 5571.437 kb on + strand at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.589 kb on + strand, within Pf1N1B4_5024 at 5571.590 kb on - strand at 5571.590 kb on - strand, within Pf1N1B4_5024
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 5,569,064 + Pf1N1B4_5019 0.72 -1.7 5,569,229 - +2.2 5,569,237 + -1.6 5,569,237 + -3.0 5,569,237 + +0.5 5,569,250 - -2.9 5,569,699 + +1.0 5,569,699 + -2.8 5,569,700 - -0.9 5,569,700 - -1.6 5,569,725 - -0.4 5,569,725 - Pf1N1B4_5021 0.14 -2.5 5,570,009 + +1.3 5,570,122 + -1.0 5,570,122 + Pf1N1B4_5022 0.17 +0.3 5,570,134 - Pf1N1B4_5022 0.18 -0.1 5,570,199 + Pf1N1B4_5022 0.27 -2.8 5,570,199 + Pf1N1B4_5022 0.27 -0.8 5,570,200 - Pf1N1B4_5022 0.28 -2.0 5,570,200 - Pf1N1B4_5022 0.28 +1.8 5,570,206 - +0.0 5,570,206 - Pf1N1B4_5022 0.28 +0.4 5,570,289 + Pf1N1B4_5022 0.40 +3.0 5,570,289 + +0.0 5,570,394 + Pf1N1B4_5022 0.55 +1.4 5,570,409 + Pf1N1B4_5022 0.57 +1.8 5,570,410 - Pf1N1B4_5022 0.57 +1.0 5,570,800 + Pf1N1B4_5023 0.14 -0.2 5,570,801 - Pf1N1B4_5023 0.14 +0.6 5,570,801 - Pf1N1B4_5023 0.14 -0.1 5,570,801 - Pf1N1B4_5023 0.14 -0.1 5,570,907 + Pf1N1B4_5023 0.30 -0.4 5,570,908 - +1.0 5,570,908 - Pf1N1B4_5023 0.30 -2.0 5,570,974 + Pf1N1B4_5023 0.40 -0.0 5,571,108 + Pf1N1B4_5023 0.59 -1.1 5,571,109 - Pf1N1B4_5023 0.59 -2.3 5,571,109 - +2.4 5,571,109 - Pf1N1B4_5023 0.59 +1.3 5,571,109 - Pf1N1B4_5023 0.59 -0.0 5,571,418 - -1.8 5,571,418 - +1.7 5,571,437 + +0.3 5,571,566 - Pf1N1B4_5024 0.11 -2.0 5,571,566 - Pf1N1B4_5024 0.11 +0.5 5,571,566 - Pf1N1B4_5024 0.11 +0.8 5,571,566 - Pf1N1B4_5024 0.11 -0.6 5,571,589 + Pf1N1B4_5024 0.13 +1.0 5,571,590 - +0.0 5,571,590 - Pf1N1B4_5024 0.13 -0.1
Or see this region's nucleotide sequence