Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4844 and Pf1N1B4_4845 are separated by 13 nucleotides Pf1N1B4_4845 and Pf1N1B4_4846 are separated by 126 nucleotides
Pf1N1B4_4844 - Fructokinase (EC 2.7.1.4), at 5,351,801 to 5,352,739
_4844
Pf1N1B4_4845 - Xylulose kinase (EC 2.7.1.17), at 5,352,753 to 5,354,249
_4845
Pf1N1B4_4846 - Multiple polyol-specific dehydrogenase (EC 1.1.1.-), at 5,354,376 to 5,355,851
_4846
Position (kb) 5352
5353
5354
5355 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5352.332 kb on + strand, within Pf1N1B4_4844 at 5352.728 kb on + strand at 5352.735 kb on + strand at 5352.736 kb on - strand at 5352.736 kb on - strand at 5352.736 kb on - strand at 5352.739 kb on + strand at 5352.739 kb on + strand at 5352.739 kb on + strand at 5352.740 kb on - strand at 5352.740 kb on - strand at 5352.740 kb on - strand at 5352.740 kb on - strand at 5352.977 kb on + strand, within Pf1N1B4_4845 at 5352.977 kb on + strand, within Pf1N1B4_4845 at 5352.978 kb on - strand, within Pf1N1B4_4845 at 5353.056 kb on + strand, within Pf1N1B4_4845 at 5353.057 kb on - strand at 5353.057 kb on - strand, within Pf1N1B4_4845 at 5353.426 kb on + strand, within Pf1N1B4_4845 at 5353.427 kb on - strand at 5353.473 kb on + strand, within Pf1N1B4_4845 at 5353.473 kb on + strand at 5353.474 kb on - strand, within Pf1N1B4_4845 at 5353.475 kb on + strand, within Pf1N1B4_4845 at 5353.714 kb on + strand, within Pf1N1B4_4845 at 5353.714 kb on + strand, within Pf1N1B4_4845 at 5353.744 kb on + strand, within Pf1N1B4_4845 at 5353.744 kb on + strand, within Pf1N1B4_4845 at 5353.745 kb on - strand at 5353.745 kb on - strand, within Pf1N1B4_4845 at 5353.753 kb on + strand, within Pf1N1B4_4845 at 5354.037 kb on + strand, within Pf1N1B4_4845 at 5354.038 kb on - strand, within Pf1N1B4_4845 at 5354.232 kb on + strand at 5354.232 kb on + strand at 5354.232 kb on + strand at 5354.233 kb on - strand at 5354.233 kb on - strand at 5354.261 kb on - strand at 5354.581 kb on + strand, within Pf1N1B4_4846 at 5354.582 kb on - strand, within Pf1N1B4_4846 at 5354.582 kb on - strand, within Pf1N1B4_4846 at 5354.582 kb on - strand, within Pf1N1B4_4846 at 5354.792 kb on - strand, within Pf1N1B4_4846 at 5354.851 kb on + strand, within Pf1N1B4_4846 at 5354.863 kb on + strand, within Pf1N1B4_4846 at 5354.864 kb on - strand, within Pf1N1B4_4846 at 5354.929 kb on + strand at 5354.930 kb on - strand, within Pf1N1B4_4846
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 5,352,332 + Pf1N1B4_4844 0.57 -1.8 5,352,728 + -0.1 5,352,735 + +0.3 5,352,736 - +2.6 5,352,736 - -0.3 5,352,736 - -1.3 5,352,739 + +0.5 5,352,739 + -0.1 5,352,739 + -2.3 5,352,740 - -0.4 5,352,740 - +0.2 5,352,740 - +0.5 5,352,740 - -1.4 5,352,977 + Pf1N1B4_4845 0.15 -3.4 5,352,977 + Pf1N1B4_4845 0.15 -0.9 5,352,978 - Pf1N1B4_4845 0.15 -0.8 5,353,056 + Pf1N1B4_4845 0.20 +0.7 5,353,057 - -1.0 5,353,057 - Pf1N1B4_4845 0.20 -1.3 5,353,426 + Pf1N1B4_4845 0.45 -2.8 5,353,427 - +0.3 5,353,473 + Pf1N1B4_4845 0.48 -1.4 5,353,473 + +0.8 5,353,474 - Pf1N1B4_4845 0.48 -3.0 5,353,475 + Pf1N1B4_4845 0.48 -0.2 5,353,714 + Pf1N1B4_4845 0.64 +0.3 5,353,714 + Pf1N1B4_4845 0.64 +0.9 5,353,744 + Pf1N1B4_4845 0.66 +0.3 5,353,744 + Pf1N1B4_4845 0.66 -1.4 5,353,745 - -2.0 5,353,745 - Pf1N1B4_4845 0.66 -1.0 5,353,753 + Pf1N1B4_4845 0.67 -1.0 5,354,037 + Pf1N1B4_4845 0.86 -2.2 5,354,038 - Pf1N1B4_4845 0.86 +0.2 5,354,232 + -0.7 5,354,232 + +0.1 5,354,232 + +0.2 5,354,233 - +0.9 5,354,233 - +0.8 5,354,261 - -1.3 5,354,581 + Pf1N1B4_4846 0.14 -0.7 5,354,582 - Pf1N1B4_4846 0.14 +0.6 5,354,582 - Pf1N1B4_4846 0.14 +0.7 5,354,582 - Pf1N1B4_4846 0.14 -1.4 5,354,792 - Pf1N1B4_4846 0.28 -0.9 5,354,851 + Pf1N1B4_4846 0.32 +1.6 5,354,863 + Pf1N1B4_4846 0.33 -0.0 5,354,864 - Pf1N1B4_4846 0.33 +0.1 5,354,929 + -0.4 5,354,930 - Pf1N1B4_4846 0.38 +0.7
Or see this region's nucleotide sequence