Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4777 and Pf1N1B4_4778 are separated by 6 nucleotides Pf1N1B4_4778 and Pf1N1B4_4779 overlap by 33 nucleotides
Pf1N1B4_4777 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 5,279,072 to 5,279,884
_4777
Pf1N1B4_4778 - NADH pyrophosphatase (EC 3.6.1.22), at 5,279,891 to 5,280,721
_4778
Pf1N1B4_4779 - Membrane protein, putative, at 5,280,689 to 5,281,468
_4779
Position (kb)
5279
5280
5281 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 5278.899 kb on + strand at 5279.031 kb on + strand at 5279.032 kb on - strand at 5279.032 kb on - strand at 5279.045 kb on + strand at 5279.046 kb on - strand at 5279.046 kb on - strand at 5279.046 kb on - strand at 5279.082 kb on - strand at 5279.530 kb on - strand, within Pf1N1B4_4777 at 5279.530 kb on - strand, within Pf1N1B4_4777 at 5279.588 kb on + strand, within Pf1N1B4_4777 at 5279.649 kb on - strand, within Pf1N1B4_4777 at 5279.750 kb on + strand, within Pf1N1B4_4777 at 5279.787 kb on - strand, within Pf1N1B4_4777 at 5279.851 kb on + strand at 5279.851 kb on + strand at 5279.852 kb on - strand at 5280.167 kb on + strand, within Pf1N1B4_4778 at 5280.168 kb on - strand, within Pf1N1B4_4778 at 5280.168 kb on - strand at 5280.275 kb on + strand, within Pf1N1B4_4778 at 5280.275 kb on + strand, within Pf1N1B4_4778 at 5280.276 kb on - strand at 5280.294 kb on + strand, within Pf1N1B4_4778 at 5280.294 kb on + strand, within Pf1N1B4_4778 at 5280.295 kb on - strand, within Pf1N1B4_4778 at 5280.295 kb on - strand, within Pf1N1B4_4778 at 5280.393 kb on - strand, within Pf1N1B4_4778 at 5280.398 kb on - strand, within Pf1N1B4_4778 at 5280.398 kb on - strand, within Pf1N1B4_4778 at 5280.494 kb on + strand, within Pf1N1B4_4778 at 5280.494 kb on + strand at 5280.494 kb on + strand at 5280.494 kb on + strand, within Pf1N1B4_4778 at 5280.494 kb on + strand, within Pf1N1B4_4778 at 5280.674 kb on + strand at 5280.674 kb on + strand at 5280.674 kb on + strand at 5280.675 kb on - strand at 5280.872 kb on + strand, within Pf1N1B4_4779 at 5281.014 kb on + strand, within Pf1N1B4_4779 at 5281.110 kb on + strand at 5281.367 kb on + strand, within Pf1N1B4_4779 at 5281.368 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 5,278,899 + +1.2 5,279,031 + +0.5 5,279,032 - -2.4 5,279,032 - -0.5 5,279,045 + -0.1 5,279,046 - +0.6 5,279,046 - +1.6 5,279,046 - -1.4 5,279,082 - -1.6 5,279,530 - Pf1N1B4_4777 0.56 +0.2 5,279,530 - Pf1N1B4_4777 0.56 -0.6 5,279,588 + Pf1N1B4_4777 0.63 -1.0 5,279,649 - Pf1N1B4_4777 0.71 -0.3 5,279,750 + Pf1N1B4_4777 0.83 +1.8 5,279,787 - Pf1N1B4_4777 0.88 +2.1 5,279,851 + -0.4 5,279,851 + -0.2 5,279,852 - +0.6 5,280,167 + Pf1N1B4_4778 0.33 +0.7 5,280,168 - Pf1N1B4_4778 0.33 -0.6 5,280,168 - -0.4 5,280,275 + Pf1N1B4_4778 0.46 +0.9 5,280,275 + Pf1N1B4_4778 0.46 +0.5 5,280,276 - -2.0 5,280,294 + Pf1N1B4_4778 0.48 +1.7 5,280,294 + Pf1N1B4_4778 0.48 +1.4 5,280,295 - Pf1N1B4_4778 0.49 -0.9 5,280,295 - Pf1N1B4_4778 0.49 +1.2 5,280,393 - Pf1N1B4_4778 0.60 +1.1 5,280,398 - Pf1N1B4_4778 0.61 +0.1 5,280,398 - Pf1N1B4_4778 0.61 +0.3 5,280,494 + Pf1N1B4_4778 0.73 +1.2 5,280,494 + +3.9 5,280,494 + +0.5 5,280,494 + Pf1N1B4_4778 0.73 -2.2 5,280,494 + Pf1N1B4_4778 0.73 -2.0 5,280,674 + -1.4 5,280,674 + -0.1 5,280,674 + +0.0 5,280,675 - -1.9 5,280,872 + Pf1N1B4_4779 0.23 -1.2 5,281,014 + Pf1N1B4_4779 0.42 +0.3 5,281,110 + -0.9 5,281,367 + Pf1N1B4_4779 0.87 +1.2 5,281,368 - +0.6
Or see this region's nucleotide sequence