Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4778

Experiment: nitrogen source nitrite 0.1mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4777 and Pf1N1B4_4778 are separated by 6 nucleotidesPf1N1B4_4778 and Pf1N1B4_4779 overlap by 33 nucleotides Pf1N1B4_4777 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 5,279,072 to 5,279,884 _4777 Pf1N1B4_4778 - NADH pyrophosphatase (EC 3.6.1.22), at 5,279,891 to 5,280,721 _4778 Pf1N1B4_4779 - Membrane protein, putative, at 5,280,689 to 5,281,468 _4779 Position (kb) 5279 5280 5281Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 5278.899 kb on + strandat 5279.031 kb on + strandat 5279.032 kb on - strandat 5279.032 kb on - strandat 5279.045 kb on + strandat 5279.046 kb on - strandat 5279.046 kb on - strandat 5279.046 kb on - strandat 5279.082 kb on - strandat 5279.530 kb on - strand, within Pf1N1B4_4777at 5279.530 kb on - strand, within Pf1N1B4_4777at 5279.588 kb on + strand, within Pf1N1B4_4777at 5279.649 kb on - strand, within Pf1N1B4_4777at 5279.750 kb on + strand, within Pf1N1B4_4777at 5279.787 kb on - strand, within Pf1N1B4_4777at 5279.851 kb on + strandat 5279.851 kb on + strandat 5279.852 kb on - strandat 5280.167 kb on + strand, within Pf1N1B4_4778at 5280.168 kb on - strand, within Pf1N1B4_4778at 5280.168 kb on - strandat 5280.275 kb on + strand, within Pf1N1B4_4778at 5280.275 kb on + strand, within Pf1N1B4_4778at 5280.276 kb on - strandat 5280.294 kb on + strand, within Pf1N1B4_4778at 5280.294 kb on + strand, within Pf1N1B4_4778at 5280.295 kb on - strand, within Pf1N1B4_4778at 5280.295 kb on - strand, within Pf1N1B4_4778at 5280.393 kb on - strand, within Pf1N1B4_4778at 5280.398 kb on - strand, within Pf1N1B4_4778at 5280.398 kb on - strand, within Pf1N1B4_4778at 5280.494 kb on + strand, within Pf1N1B4_4778at 5280.494 kb on + strandat 5280.494 kb on + strandat 5280.494 kb on + strand, within Pf1N1B4_4778at 5280.494 kb on + strand, within Pf1N1B4_4778at 5280.674 kb on + strandat 5280.674 kb on + strandat 5280.674 kb on + strandat 5280.675 kb on - strandat 5280.872 kb on + strand, within Pf1N1B4_4779at 5281.014 kb on + strand, within Pf1N1B4_4779at 5281.110 kb on + strandat 5281.367 kb on + strand, within Pf1N1B4_4779at 5281.368 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM
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5,278,899 + +1.2
5,279,031 + +0.5
5,279,032 - -2.4
5,279,032 - -0.5
5,279,045 + -0.1
5,279,046 - +0.6
5,279,046 - +1.6
5,279,046 - -1.4
5,279,082 - -1.6
5,279,530 - Pf1N1B4_4777 0.56 +0.2
5,279,530 - Pf1N1B4_4777 0.56 -0.6
5,279,588 + Pf1N1B4_4777 0.63 -1.0
5,279,649 - Pf1N1B4_4777 0.71 -0.3
5,279,750 + Pf1N1B4_4777 0.83 +1.8
5,279,787 - Pf1N1B4_4777 0.88 +2.1
5,279,851 + -0.4
5,279,851 + -0.2
5,279,852 - +0.6
5,280,167 + Pf1N1B4_4778 0.33 +0.7
5,280,168 - Pf1N1B4_4778 0.33 -0.6
5,280,168 - -0.4
5,280,275 + Pf1N1B4_4778 0.46 +0.9
5,280,275 + Pf1N1B4_4778 0.46 +0.5
5,280,276 - -2.0
5,280,294 + Pf1N1B4_4778 0.48 +1.7
5,280,294 + Pf1N1B4_4778 0.48 +1.4
5,280,295 - Pf1N1B4_4778 0.49 -0.9
5,280,295 - Pf1N1B4_4778 0.49 +1.2
5,280,393 - Pf1N1B4_4778 0.60 +1.1
5,280,398 - Pf1N1B4_4778 0.61 +0.1
5,280,398 - Pf1N1B4_4778 0.61 +0.3
5,280,494 + Pf1N1B4_4778 0.73 +1.2
5,280,494 + +3.9
5,280,494 + +0.5
5,280,494 + Pf1N1B4_4778 0.73 -2.2
5,280,494 + Pf1N1B4_4778 0.73 -2.0
5,280,674 + -1.4
5,280,674 + -0.1
5,280,674 + +0.0
5,280,675 - -1.9
5,280,872 + Pf1N1B4_4779 0.23 -1.2
5,281,014 + Pf1N1B4_4779 0.42 +0.3
5,281,110 + -0.9
5,281,367 + Pf1N1B4_4779 0.87 +1.2
5,281,368 - +0.6

Or see this region's nucleotide sequence