Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4770 and Pf1N1B4_4771 are separated by 101 nucleotides Pf1N1B4_4771 and Pf1N1B4_4772 are separated by 190 nucleotides
Pf1N1B4_4770 - Putative autotransporter, at 5,268,195 to 5,270,408
_4770
Pf1N1B4_4771 - Probable transmembrane protein, at 5,270,510 to 5,271,151
_4771
Pf1N1B4_4772 - RND efflux system, inner membrane transporter CmeB, at 5,271,342 to 5,274,440
_4772
Position (kb) 5270
5271
5272 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5269.642 kb on - strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.948 kb on - strand, within Pf1N1B4_4770 at 5269.991 kb on + strand, within Pf1N1B4_4770 at 5270.010 kb on + strand, within Pf1N1B4_4770 at 5270.010 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.035 kb on + strand, within Pf1N1B4_4770 at 5270.267 kb on + strand at 5270.315 kb on + strand at 5270.315 kb on + strand at 5270.315 kb on + strand at 5270.386 kb on + strand at 5270.386 kb on + strand at 5270.386 kb on + strand at 5270.386 kb on + strand at 5270.397 kb on + strand at 5270.398 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.745 kb on + strand, within Pf1N1B4_4771 at 5270.745 kb on + strand at 5270.746 kb on - strand, within Pf1N1B4_4771 at 5270.748 kb on + strand, within Pf1N1B4_4771 at 5270.748 kb on + strand at 5270.749 kb on - strand, within Pf1N1B4_4771 at 5270.749 kb on - strand at 5270.760 kb on + strand, within Pf1N1B4_4771 at 5270.761 kb on - strand, within Pf1N1B4_4771 at 5270.927 kb on + strand at 5270.927 kb on + strand at 5270.927 kb on + strand, within Pf1N1B4_4771 at 5271.177 kb on - strand at 5271.602 kb on - strand at 5271.894 kb on + strand, within Pf1N1B4_4772 at 5272.083 kb on - strand, within Pf1N1B4_4772 at 5272.083 kb on - strand, within Pf1N1B4_4772 at 5272.083 kb on - strand, within Pf1N1B4_4772 at 5272.083 kb on - strand, within Pf1N1B4_4772 at 5272.087 kb on - strand at 5272.087 kb on - strand, within Pf1N1B4_4772
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 5,269,642 - Pf1N1B4_4770 0.65 -1.2 5,269,947 + Pf1N1B4_4770 0.79 +1.0 5,269,947 + Pf1N1B4_4770 0.79 +0.1 5,269,947 + Pf1N1B4_4770 0.79 -0.5 5,269,947 + Pf1N1B4_4770 0.79 +2.0 5,269,948 - Pf1N1B4_4770 0.79 +0.2 5,269,991 + Pf1N1B4_4770 0.81 +1.5 5,270,010 + Pf1N1B4_4770 0.82 -0.9 5,270,010 + Pf1N1B4_4770 0.82 -1.7 5,270,029 + Pf1N1B4_4770 0.83 -2.6 5,270,029 + +0.3 5,270,029 + Pf1N1B4_4770 0.83 +1.0 5,270,029 + Pf1N1B4_4770 0.83 -1.7 5,270,029 + Pf1N1B4_4770 0.83 +0.2 5,270,035 + Pf1N1B4_4770 0.83 +1.6 5,270,267 + -0.6 5,270,315 + +0.6 5,270,315 + -1.1 5,270,315 + +2.0 5,270,386 + +0.6 5,270,386 + +0.2 5,270,386 + -0.8 5,270,386 + -1.7 5,270,397 + +2.1 5,270,398 - +0.1 5,270,561 - -1.4 5,270,561 - -1.4 5,270,561 - +0.7 5,270,561 - -2.1 5,270,561 - -0.2 5,270,745 + Pf1N1B4_4771 0.37 +0.5 5,270,745 + -2.3 5,270,746 - Pf1N1B4_4771 0.37 +1.1 5,270,748 + Pf1N1B4_4771 0.37 -3.2 5,270,748 + -0.1 5,270,749 - Pf1N1B4_4771 0.37 +1.1 5,270,749 - +1.0 5,270,760 + Pf1N1B4_4771 0.39 +0.8 5,270,761 - Pf1N1B4_4771 0.39 +1.5 5,270,927 + -1.2 5,270,927 + +2.6 5,270,927 + Pf1N1B4_4771 0.65 +0.3 5,271,177 - +0.9 5,271,602 - +0.8 5,271,894 + Pf1N1B4_4772 0.18 +0.6 5,272,083 - Pf1N1B4_4772 0.24 -0.0 5,272,083 - Pf1N1B4_4772 0.24 +0.8 5,272,083 - Pf1N1B4_4772 0.24 -1.3 5,272,083 - Pf1N1B4_4772 0.24 -1.3 5,272,087 - -0.7 5,272,087 - Pf1N1B4_4772 0.24 +0.0
Or see this region's nucleotide sequence