Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4353 and Pf1N1B4_4354 are separated by 280 nucleotides Pf1N1B4_4354 and Pf1N1B4_4355 are separated by 234 nucleotides
Pf1N1B4_4353 - Gamma-glutamyl-GABA hydrolase (EC 3.5.1.94), at 4,809,479 to 4,810,240
_4353
Pf1N1B4_4354 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11), at 4,810,521 to 4,811,897
_4354
Pf1N1B4_4355 - Gamma-glutamyl-aminobutyraldehyde dehydrogenase (EC 1.2.1.-), at 4,812,132 to 4,813,622
_4355
Position (kb) 4810
4811
4812 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4809.538 kb on + strand at 4809.546 kb on + strand at 4810.159 kb on - strand, within Pf1N1B4_4353 at 4810.413 kb on + strand at 4810.414 kb on - strand at 4810.593 kb on + strand at 4810.594 kb on - strand at 4810.665 kb on - strand, within Pf1N1B4_4354 at 4810.672 kb on - strand at 4810.968 kb on + strand, within Pf1N1B4_4354 at 4811.056 kb on - strand, within Pf1N1B4_4354 at 4811.201 kb on + strand, within Pf1N1B4_4354 at 4811.201 kb on + strand, within Pf1N1B4_4354 at 4811.202 kb on - strand, within Pf1N1B4_4354 at 4811.202 kb on - strand, within Pf1N1B4_4354 at 4811.203 kb on + strand, within Pf1N1B4_4354 at 4811.203 kb on + strand, within Pf1N1B4_4354 at 4811.257 kb on - strand, within Pf1N1B4_4354 at 4811.496 kb on + strand, within Pf1N1B4_4354 at 4811.496 kb on + strand, within Pf1N1B4_4354 at 4811.497 kb on - strand, within Pf1N1B4_4354 at 4811.497 kb on - strand, within Pf1N1B4_4354 at 4811.704 kb on - strand, within Pf1N1B4_4354 at 4811.784 kb on + strand at 4811.784 kb on + strand at 4811.784 kb on + strand at 4811.799 kb on + strand at 4811.799 kb on + strand at 4811.871 kb on + strand at 4811.871 kb on + strand at 4811.871 kb on + strand at 4811.872 kb on - strand at 4811.872 kb on - strand at 4811.880 kb on + strand at 4811.880 kb on + strand at 4812.024 kb on - strand at 4812.103 kb on + strand at 4812.103 kb on + strand at 4812.103 kb on + strand at 4812.103 kb on + strand at 4812.103 kb on + strand at 4812.103 kb on + strand at 4812.103 kb on + strand at 4812.103 kb on + strand at 4812.104 kb on - strand at 4812.104 kb on - strand at 4812.107 kb on + strand at 4812.107 kb on + strand at 4812.107 kb on + strand at 4812.107 kb on + strand at 4812.108 kb on - strand at 4812.181 kb on + strand at 4812.181 kb on + strand at 4812.182 kb on - strand at 4812.361 kb on + strand, within Pf1N1B4_4355 at 4812.544 kb on - strand, within Pf1N1B4_4355
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 4,809,538 + -2.7 4,809,546 + +1.1 4,810,159 - Pf1N1B4_4353 0.89 +1.2 4,810,413 + -3.3 4,810,414 - +1.6 4,810,593 + -2.0 4,810,594 - -0.9 4,810,665 - Pf1N1B4_4354 0.10 +2.2 4,810,672 - -1.6 4,810,968 + Pf1N1B4_4354 0.32 +0.5 4,811,056 - Pf1N1B4_4354 0.39 +1.1 4,811,201 + Pf1N1B4_4354 0.49 +0.4 4,811,201 + Pf1N1B4_4354 0.49 +1.1 4,811,202 - Pf1N1B4_4354 0.49 +0.5 4,811,202 - Pf1N1B4_4354 0.49 +0.1 4,811,203 + Pf1N1B4_4354 0.50 -0.4 4,811,203 + Pf1N1B4_4354 0.50 +1.7 4,811,257 - Pf1N1B4_4354 0.53 -2.1 4,811,496 + Pf1N1B4_4354 0.71 +0.9 4,811,496 + Pf1N1B4_4354 0.71 +0.3 4,811,497 - Pf1N1B4_4354 0.71 -0.6 4,811,497 - Pf1N1B4_4354 0.71 +1.4 4,811,704 - Pf1N1B4_4354 0.86 +2.0 4,811,784 + -0.4 4,811,784 + +0.3 4,811,784 + +2.2 4,811,799 + -0.4 4,811,799 + +1.5 4,811,871 + +0.3 4,811,871 + +1.4 4,811,871 + -0.3 4,811,872 - +0.6 4,811,872 - +0.7 4,811,880 + +2.6 4,811,880 + +0.4 4,812,024 - +1.7 4,812,103 + -1.2 4,812,103 + +1.0 4,812,103 + +0.8 4,812,103 + -1.0 4,812,103 + +0.3 4,812,103 + -0.2 4,812,103 + -2.1 4,812,103 + +0.6 4,812,104 - -1.1 4,812,104 - -1.2 4,812,107 + -1.7 4,812,107 + -0.6 4,812,107 + +0.9 4,812,107 + -1.6 4,812,108 - -0.9 4,812,181 + -1.1 4,812,181 + -2.4 4,812,182 - +0.4 4,812,361 + Pf1N1B4_4355 0.15 +1.4 4,812,544 - Pf1N1B4_4355 0.28 -0.7
Or see this region's nucleotide sequence