Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4214 and Pf1N1B4_4215 are separated by 456 nucleotides Pf1N1B4_4215 and Pf1N1B4_4216 are separated by 258 nucleotides
Pf1N1B4_4214 - Arginine/ornithine antiporter ArcD, at 4,667,923 to 4,669,389
_4214
Pf1N1B4_4215 - Adenylate cyclase (EC 4.6.1.1), at 4,669,846 to 4,671,435
_4215
Pf1N1B4_4216 - Putative secreted protein, at 4,671,694 to 4,672,446
_4216
Position (kb) 4669
4670
4671
4672 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4668.891 kb on + strand, within Pf1N1B4_4214 at 4669.127 kb on + strand, within Pf1N1B4_4214 at 4669.128 kb on - strand, within Pf1N1B4_4214 at 4669.249 kb on - strand at 4669.249 kb on - strand at 4669.332 kb on + strand at 4669.333 kb on - strand at 4669.684 kb on + strand at 4669.685 kb on - strand at 4669.697 kb on + strand at 4669.698 kb on - strand at 4669.698 kb on - strand at 4669.699 kb on + strand at 4670.185 kb on + strand, within Pf1N1B4_4215 at 4670.522 kb on + strand, within Pf1N1B4_4215 at 4670.522 kb on + strand, within Pf1N1B4_4215 at 4670.522 kb on + strand, within Pf1N1B4_4215 at 4670.522 kb on + strand, within Pf1N1B4_4215 at 4670.523 kb on - strand, within Pf1N1B4_4215 at 4670.600 kb on + strand, within Pf1N1B4_4215 at 4670.751 kb on + strand, within Pf1N1B4_4215 at 4670.751 kb on + strand, within Pf1N1B4_4215 at 4670.751 kb on + strand, within Pf1N1B4_4215 at 4670.752 kb on - strand, within Pf1N1B4_4215 at 4670.752 kb on - strand, within Pf1N1B4_4215 at 4670.752 kb on - strand at 4670.794 kb on + strand, within Pf1N1B4_4215 at 4670.820 kb on + strand, within Pf1N1B4_4215 at 4670.886 kb on + strand, within Pf1N1B4_4215 at 4670.888 kb on + strand, within Pf1N1B4_4215 at 4670.888 kb on + strand, within Pf1N1B4_4215 at 4670.888 kb on + strand, within Pf1N1B4_4215 at 4670.888 kb on + strand, within Pf1N1B4_4215 at 4670.889 kb on - strand, within Pf1N1B4_4215 at 4670.889 kb on - strand, within Pf1N1B4_4215 at 4670.889 kb on - strand, within Pf1N1B4_4215 at 4670.979 kb on - strand, within Pf1N1B4_4215 at 4671.337 kb on + strand at 4671.337 kb on + strand at 4671.338 kb on - strand at 4671.413 kb on + strand at 4671.413 kb on + strand at 4671.413 kb on + strand at 4671.414 kb on - strand at 4671.414 kb on - strand at 4671.804 kb on + strand, within Pf1N1B4_4216 at 4671.804 kb on + strand at 4672.067 kb on - strand, within Pf1N1B4_4216
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 4,668,891 + Pf1N1B4_4214 0.66 +1.4 4,669,127 + Pf1N1B4_4214 0.82 +0.7 4,669,128 - Pf1N1B4_4214 0.82 -1.6 4,669,249 - +0.5 4,669,249 - -0.8 4,669,332 + -1.4 4,669,333 - -0.1 4,669,684 + +1.4 4,669,685 - +0.9 4,669,697 + +1.8 4,669,698 - -1.0 4,669,698 - -2.3 4,669,699 + +0.3 4,670,185 + Pf1N1B4_4215 0.21 +2.2 4,670,522 + Pf1N1B4_4215 0.43 +0.5 4,670,522 + Pf1N1B4_4215 0.43 +0.8 4,670,522 + Pf1N1B4_4215 0.43 -2.6 4,670,522 + Pf1N1B4_4215 0.43 +0.4 4,670,523 - Pf1N1B4_4215 0.43 -2.0 4,670,600 + Pf1N1B4_4215 0.47 +1.1 4,670,751 + Pf1N1B4_4215 0.57 +0.6 4,670,751 + Pf1N1B4_4215 0.57 -0.4 4,670,751 + Pf1N1B4_4215 0.57 +1.4 4,670,752 - Pf1N1B4_4215 0.57 -0.4 4,670,752 - Pf1N1B4_4215 0.57 +1.3 4,670,752 - -2.0 4,670,794 + Pf1N1B4_4215 0.60 +1.6 4,670,820 + Pf1N1B4_4215 0.61 +0.4 4,670,886 + Pf1N1B4_4215 0.65 +1.8 4,670,888 + Pf1N1B4_4215 0.66 -1.8 4,670,888 + Pf1N1B4_4215 0.66 -1.8 4,670,888 + Pf1N1B4_4215 0.66 +2.1 4,670,888 + Pf1N1B4_4215 0.66 +0.6 4,670,889 - Pf1N1B4_4215 0.66 +0.2 4,670,889 - Pf1N1B4_4215 0.66 +0.8 4,670,889 - Pf1N1B4_4215 0.66 +1.8 4,670,979 - Pf1N1B4_4215 0.71 +2.2 4,671,337 + +0.9 4,671,337 + +0.0 4,671,338 - +1.3 4,671,413 + +1.9 4,671,413 + -0.8 4,671,413 + +0.7 4,671,414 - +1.5 4,671,414 - -0.1 4,671,804 + Pf1N1B4_4216 0.15 +0.1 4,671,804 + -1.9 4,672,067 - Pf1N1B4_4216 0.50 +0.3
Or see this region's nucleotide sequence