Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4104 and Pf1N1B4_4105 are separated by 47 nucleotides Pf1N1B4_4105 and Pf1N1B4_4106 are separated by 93 nucleotides Pf1N1B4_4106 and Pf1N1B4_4107 are separated by 240 nucleotides Pf1N1B4_4107 and Pf1N1B4_4108 are separated by 15 nucleotides
Pf1N1B4_4104 - Probable transmembrane protein, at 4,552,366 to 4,552,851
_4104
Pf1N1B4_4105 - hypothetical protein, at 4,552,899 to 4,553,366
_4105
Pf1N1B4_4106 - Mesenchymal stem cell protein DSCD75, at 4,553,460 to 4,554,032
_4106
Pf1N1B4_4107 - Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog, at 4,554,273 to 4,554,905
_4107
Pf1N1B4_4108 - Hydrolase, alpha/beta fold family, at 4,554,921 to 4,555,718
_4108
Position (kb)
4553
4554
4555 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4552.567 kb on + strand, within Pf1N1B4_4104 at 4552.567 kb on + strand, within Pf1N1B4_4104 at 4552.568 kb on - strand, within Pf1N1B4_4104 at 4552.568 kb on - strand, within Pf1N1B4_4104 at 4552.765 kb on - strand, within Pf1N1B4_4104 at 4553.212 kb on + strand, within Pf1N1B4_4105 at 4553.212 kb on + strand, within Pf1N1B4_4105 at 4553.213 kb on - strand at 4553.213 kb on - strand, within Pf1N1B4_4105 at 4553.386 kb on - strand at 4553.436 kb on + strand at 4553.440 kb on - strand at 4553.440 kb on - strand at 4553.446 kb on + strand at 4553.525 kb on + strand, within Pf1N1B4_4106 at 4553.549 kb on + strand, within Pf1N1B4_4106 at 4553.550 kb on - strand, within Pf1N1B4_4106 at 4553.550 kb on - strand at 4553.730 kb on + strand, within Pf1N1B4_4106 at 4553.731 kb on - strand at 4553.739 kb on - strand at 4553.871 kb on + strand, within Pf1N1B4_4106 at 4553.872 kb on - strand, within Pf1N1B4_4106 at 4553.872 kb on - strand, within Pf1N1B4_4106 at 4553.883 kb on - strand at 4553.890 kb on + strand, within Pf1N1B4_4106 at 4554.053 kb on - strand at 4554.053 kb on - strand at 4554.053 kb on - strand at 4554.057 kb on - strand at 4554.057 kb on - strand at 4554.091 kb on - strand at 4554.420 kb on + strand, within Pf1N1B4_4107 at 4554.738 kb on + strand, within Pf1N1B4_4107 at 4554.738 kb on + strand, within Pf1N1B4_4107 at 4554.738 kb on + strand, within Pf1N1B4_4107 at 4554.903 kb on + strand at 4554.935 kb on + strand at 4554.935 kb on + strand at 4554.974 kb on + strand at 4554.986 kb on + strand at 4554.986 kb on + strand at 4554.986 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 4,552,567 + Pf1N1B4_4104 0.41 -0.5 4,552,567 + Pf1N1B4_4104 0.41 +1.2 4,552,568 - Pf1N1B4_4104 0.42 +0.1 4,552,568 - Pf1N1B4_4104 0.42 -0.3 4,552,765 - Pf1N1B4_4104 0.82 +0.4 4,553,212 + Pf1N1B4_4105 0.67 +1.5 4,553,212 + Pf1N1B4_4105 0.67 +0.1 4,553,213 - +0.2 4,553,213 - Pf1N1B4_4105 0.67 -0.8 4,553,386 - -0.1 4,553,436 + +0.5 4,553,440 - +0.8 4,553,440 - +1.1 4,553,446 + -1.1 4,553,525 + Pf1N1B4_4106 0.11 -2.7 4,553,549 + Pf1N1B4_4106 0.16 -0.1 4,553,550 - Pf1N1B4_4106 0.16 -1.5 4,553,550 - +3.1 4,553,730 + Pf1N1B4_4106 0.47 +0.9 4,553,731 - +0.3 4,553,739 - -1.5 4,553,871 + Pf1N1B4_4106 0.72 +0.4 4,553,872 - Pf1N1B4_4106 0.72 -2.4 4,553,872 - Pf1N1B4_4106 0.72 -2.4 4,553,883 - +0.8 4,553,890 + Pf1N1B4_4106 0.75 -2.6 4,554,053 - -0.6 4,554,053 - -0.4 4,554,053 - -0.4 4,554,057 - +0.6 4,554,057 - -0.9 4,554,091 - +0.7 4,554,420 + Pf1N1B4_4107 0.23 -0.4 4,554,738 + Pf1N1B4_4107 0.73 -1.1 4,554,738 + Pf1N1B4_4107 0.73 +0.2 4,554,738 + Pf1N1B4_4107 0.73 -1.5 4,554,903 + +0.5 4,554,935 + +1.1 4,554,935 + -1.7 4,554,974 + +1.3 4,554,986 + +0.2 4,554,986 + -2.2 4,554,986 + -0.4
Or see this region's nucleotide sequence