Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3744 and Pf1N1B4_3745 overlap by 4 nucleotides Pf1N1B4_3745 and Pf1N1B4_3746 overlap by 14 nucleotides Pf1N1B4_3746 and Pf1N1B4_3747 overlap by 4 nucleotides Pf1N1B4_3747 and Pf1N1B4_3748 are separated by 179 nucleotides
Pf1N1B4_3744 - ABC transporter, ATP-binding protein, at 4,155,693 to 4,156,682
_3744
Pf1N1B4_3745 - Thiamin ABC transporter, transmembrane component, at 4,156,679 to 4,157,479
_3745
Pf1N1B4_3746 - ABC transporter permease protein, at 4,157,466 to 4,158,311
_3746
Pf1N1B4_3747 - FIG00956482: hypothetical protein, at 4,158,308 to 4,159,114
_3747
Pf1N1B4_3748 - ABC-type Fe3+ transport system, periplasmic component, at 4,159,294 to 4,160,358
_3748
Position (kb)
4157
4158
4159 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4156.630 kb on - strand at 4156.630 kb on - strand at 4156.634 kb on + strand at 4156.634 kb on + strand at 4156.635 kb on - strand at 4156.635 kb on - strand at 4156.675 kb on + strand at 4156.676 kb on - strand at 4156.676 kb on - strand at 4156.676 kb on - strand at 4156.706 kb on - strand at 4156.706 kb on - strand at 4156.743 kb on + strand at 4156.783 kb on + strand, within Pf1N1B4_3745 at 4156.792 kb on + strand, within Pf1N1B4_3745 at 4156.792 kb on + strand, within Pf1N1B4_3745 at 4156.801 kb on - strand, within Pf1N1B4_3745 at 4157.049 kb on + strand, within Pf1N1B4_3745 at 4157.049 kb on + strand, within Pf1N1B4_3745 at 4157.050 kb on - strand, within Pf1N1B4_3745 at 4157.050 kb on - strand at 4157.320 kb on - strand, within Pf1N1B4_3745 at 4157.332 kb on + strand, within Pf1N1B4_3745 at 4157.379 kb on + strand, within Pf1N1B4_3745 at 4157.380 kb on - strand, within Pf1N1B4_3745 at 4157.427 kb on + strand at 4157.427 kb on + strand at 4157.427 kb on + strand at 4157.427 kb on + strand at 4157.427 kb on + strand at 4157.428 kb on - strand at 4157.446 kb on - strand at 4157.449 kb on + strand at 4157.482 kb on + strand at 4157.482 kb on + strand at 4157.482 kb on + strand at 4157.483 kb on - strand at 4157.647 kb on + strand, within Pf1N1B4_3746 at 4157.689 kb on - strand, within Pf1N1B4_3746 at 4157.848 kb on + strand, within Pf1N1B4_3746 at 4157.849 kb on - strand, within Pf1N1B4_3746 at 4157.849 kb on - strand, within Pf1N1B4_3746 at 4157.854 kb on + strand, within Pf1N1B4_3746 at 4157.854 kb on + strand, within Pf1N1B4_3746 at 4157.855 kb on - strand, within Pf1N1B4_3746 at 4157.855 kb on - strand, within Pf1N1B4_3746 at 4157.855 kb on - strand, within Pf1N1B4_3746 at 4158.137 kb on - strand, within Pf1N1B4_3746 at 4158.137 kb on - strand, within Pf1N1B4_3746 at 4158.137 kb on - strand, within Pf1N1B4_3746 at 4158.291 kb on - strand at 4158.522 kb on + strand, within Pf1N1B4_3747 at 4158.732 kb on + strand, within Pf1N1B4_3747 at 4158.810 kb on + strand, within Pf1N1B4_3747 at 4158.810 kb on + strand, within Pf1N1B4_3747 at 4158.810 kb on + strand, within Pf1N1B4_3747 at 4158.810 kb on + strand, within Pf1N1B4_3747 at 4158.810 kb on + strand, within Pf1N1B4_3747 at 4158.810 kb on + strand, within Pf1N1B4_3747 at 4158.810 kb on + strand, within Pf1N1B4_3747 at 4158.811 kb on - strand at 4158.831 kb on + strand, within Pf1N1B4_3747 at 4158.871 kb on - strand, within Pf1N1B4_3747 at 4158.876 kb on + strand, within Pf1N1B4_3747 at 4158.877 kb on - strand, within Pf1N1B4_3747 at 4158.963 kb on + strand, within Pf1N1B4_3747 at 4158.963 kb on + strand, within Pf1N1B4_3747 at 4159.005 kb on + strand, within Pf1N1B4_3747 at 4159.006 kb on - strand, within Pf1N1B4_3747 at 4159.006 kb on - strand, within Pf1N1B4_3747 at 4159.006 kb on - strand, within Pf1N1B4_3747 at 4159.032 kb on + strand at 4159.071 kb on + strand at 4159.149 kb on + strand at 4159.149 kb on + strand at 4159.221 kb on + strand at 4159.221 kb on + strand at 4159.221 kb on + strand at 4159.221 kb on + strand at 4159.222 kb on - strand at 4159.296 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 4,156,630 - +0.1 4,156,630 - +0.3 4,156,634 + -0.4 4,156,634 + +0.4 4,156,635 - -0.6 4,156,635 - +0.7 4,156,675 + -0.3 4,156,676 - +0.4 4,156,676 - -0.5 4,156,676 - +2.5 4,156,706 - +0.5 4,156,706 - +1.4 4,156,743 + +1.6 4,156,783 + Pf1N1B4_3745 0.13 +0.3 4,156,792 + Pf1N1B4_3745 0.14 -2.1 4,156,792 + Pf1N1B4_3745 0.14 -0.3 4,156,801 - Pf1N1B4_3745 0.15 -0.2 4,157,049 + Pf1N1B4_3745 0.46 -1.8 4,157,049 + Pf1N1B4_3745 0.46 -0.4 4,157,050 - Pf1N1B4_3745 0.46 -2.3 4,157,050 - +0.6 4,157,320 - Pf1N1B4_3745 0.80 -0.3 4,157,332 + Pf1N1B4_3745 0.82 +1.1 4,157,379 + Pf1N1B4_3745 0.87 +0.7 4,157,380 - Pf1N1B4_3745 0.88 -0.1 4,157,427 + -0.1 4,157,427 + +0.9 4,157,427 + +1.4 4,157,427 + -0.7 4,157,427 + +0.3 4,157,428 - -2.6 4,157,446 - -1.1 4,157,449 + -1.2 4,157,482 + +1.7 4,157,482 + -2.3 4,157,482 + -1.7 4,157,483 - -2.4 4,157,647 + Pf1N1B4_3746 0.21 +0.6 4,157,689 - Pf1N1B4_3746 0.26 +2.4 4,157,848 + Pf1N1B4_3746 0.45 +0.3 4,157,849 - Pf1N1B4_3746 0.45 +0.0 4,157,849 - Pf1N1B4_3746 0.45 +0.6 4,157,854 + Pf1N1B4_3746 0.46 -0.6 4,157,854 + Pf1N1B4_3746 0.46 +1.3 4,157,855 - Pf1N1B4_3746 0.46 +0.9 4,157,855 - Pf1N1B4_3746 0.46 +0.5 4,157,855 - Pf1N1B4_3746 0.46 +0.6 4,158,137 - Pf1N1B4_3746 0.79 +0.9 4,158,137 - Pf1N1B4_3746 0.79 +1.9 4,158,137 - Pf1N1B4_3746 0.79 +1.2 4,158,291 - +0.6 4,158,522 + Pf1N1B4_3747 0.27 -0.8 4,158,732 + Pf1N1B4_3747 0.53 -2.4 4,158,810 + Pf1N1B4_3747 0.62 +1.5 4,158,810 + Pf1N1B4_3747 0.62 -1.4 4,158,810 + Pf1N1B4_3747 0.62 -2.5 4,158,810 + Pf1N1B4_3747 0.62 +1.4 4,158,810 + Pf1N1B4_3747 0.62 -1.0 4,158,810 + Pf1N1B4_3747 0.62 -0.3 4,158,810 + Pf1N1B4_3747 0.62 +0.2 4,158,811 - +0.7 4,158,831 + Pf1N1B4_3747 0.65 +0.9 4,158,871 - Pf1N1B4_3747 0.70 -1.4 4,158,876 + Pf1N1B4_3747 0.70 +1.1 4,158,877 - Pf1N1B4_3747 0.71 +0.1 4,158,963 + Pf1N1B4_3747 0.81 -3.5 4,158,963 + Pf1N1B4_3747 0.81 -1.2 4,159,005 + Pf1N1B4_3747 0.86 +1.6 4,159,006 - Pf1N1B4_3747 0.86 -0.4 4,159,006 - Pf1N1B4_3747 0.86 +0.2 4,159,006 - Pf1N1B4_3747 0.86 +0.8 4,159,032 + -1.1 4,159,071 + -3.0 4,159,149 + -1.2 4,159,149 + +1.4 4,159,221 + +0.2 4,159,221 + +0.1 4,159,221 + -0.2 4,159,221 + +0.1 4,159,222 - -1.7 4,159,296 - -2.1
Or see this region's nucleotide sequence