Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3692 and Pf1N1B4_3693 are separated by 145 nucleotides Pf1N1B4_3693 and Pf1N1B4_3694 are separated by 61 nucleotides Pf1N1B4_3694 and Pf1N1B4_3695 are separated by 286 nucleotides
Pf1N1B4_3692 - Chaperone protein HtpG, at 4,100,696 to 4,102,606
_3692
Pf1N1B4_3693 - Pirin, at 4,102,752 to 4,103,639
_3693
Pf1N1B4_3694 - FIG00964480: hypothetical protein, at 4,103,701 to 4,104,117
_3694
Pf1N1B4_3695 - Helicase subunit of the DNA excision repair complex, at 4,104,404 to 4,104,868
_3695
Position (kb)
4102
4103
4104 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4101.830 kb on + strand, within Pf1N1B4_3692 at 4102.011 kb on - strand, within Pf1N1B4_3692 at 4102.011 kb on - strand, within Pf1N1B4_3692 at 4102.011 kb on - strand, within Pf1N1B4_3692 at 4102.013 kb on + strand, within Pf1N1B4_3692 at 4102.563 kb on - strand at 4102.587 kb on + strand at 4102.754 kb on - strand at 4103.047 kb on + strand at 4103.047 kb on + strand, within Pf1N1B4_3693 at 4103.047 kb on + strand, within Pf1N1B4_3693 at 4103.048 kb on - strand, within Pf1N1B4_3693 at 4103.048 kb on - strand, within Pf1N1B4_3693 at 4103.048 kb on - strand, within Pf1N1B4_3693 at 4103.048 kb on - strand, within Pf1N1B4_3693 at 4103.048 kb on - strand, within Pf1N1B4_3693 at 4103.116 kb on + strand, within Pf1N1B4_3693 at 4103.117 kb on - strand, within Pf1N1B4_3693 at 4103.117 kb on - strand, within Pf1N1B4_3693 at 4103.182 kb on - strand, within Pf1N1B4_3693 at 4103.182 kb on - strand, within Pf1N1B4_3693 at 4103.236 kb on + strand, within Pf1N1B4_3693 at 4103.236 kb on + strand, within Pf1N1B4_3693 at 4103.236 kb on + strand, within Pf1N1B4_3693 at 4103.237 kb on - strand, within Pf1N1B4_3693 at 4103.301 kb on - strand, within Pf1N1B4_3693 at 4103.444 kb on - strand, within Pf1N1B4_3693 at 4103.444 kb on - strand, within Pf1N1B4_3693 at 4103.444 kb on - strand, within Pf1N1B4_3693 at 4103.858 kb on + strand, within Pf1N1B4_3694 at 4103.859 kb on - strand, within Pf1N1B4_3694 at 4103.939 kb on + strand, within Pf1N1B4_3694 at 4104.117 kb on + strand at 4104.172 kb on + strand at 4104.172 kb on + strand at 4104.173 kb on - strand at 4104.173 kb on - strand at 4104.174 kb on + strand at 4104.175 kb on - strand at 4104.175 kb on - strand at 4104.224 kb on + strand at 4104.241 kb on - strand at 4104.371 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 4,101,830 + Pf1N1B4_3692 0.59 +1.7 4,102,011 - Pf1N1B4_3692 0.69 -0.1 4,102,011 - Pf1N1B4_3692 0.69 +0.2 4,102,011 - Pf1N1B4_3692 0.69 -0.6 4,102,013 + Pf1N1B4_3692 0.69 -0.5 4,102,563 - +1.1 4,102,587 + -0.5 4,102,754 - +0.3 4,103,047 + +2.1 4,103,047 + Pf1N1B4_3693 0.33 +1.9 4,103,047 + Pf1N1B4_3693 0.33 +0.8 4,103,048 - Pf1N1B4_3693 0.33 +2.1 4,103,048 - Pf1N1B4_3693 0.33 -0.1 4,103,048 - Pf1N1B4_3693 0.33 -0.4 4,103,048 - Pf1N1B4_3693 0.33 -1.2 4,103,048 - Pf1N1B4_3693 0.33 -0.5 4,103,116 + Pf1N1B4_3693 0.41 -1.7 4,103,117 - Pf1N1B4_3693 0.41 +1.2 4,103,117 - Pf1N1B4_3693 0.41 -0.9 4,103,182 - Pf1N1B4_3693 0.48 -1.0 4,103,182 - Pf1N1B4_3693 0.48 +1.0 4,103,236 + Pf1N1B4_3693 0.55 -0.0 4,103,236 + Pf1N1B4_3693 0.55 +0.9 4,103,236 + Pf1N1B4_3693 0.55 +1.6 4,103,237 - Pf1N1B4_3693 0.55 +0.9 4,103,301 - Pf1N1B4_3693 0.62 +0.5 4,103,444 - Pf1N1B4_3693 0.78 +1.5 4,103,444 - Pf1N1B4_3693 0.78 +1.2 4,103,444 - Pf1N1B4_3693 0.78 +0.3 4,103,858 + Pf1N1B4_3694 0.38 -1.8 4,103,859 - Pf1N1B4_3694 0.38 +1.7 4,103,939 + Pf1N1B4_3694 0.57 +0.5 4,104,117 + +1.2 4,104,172 + +0.9 4,104,172 + +0.6 4,104,173 - -1.8 4,104,173 - -0.9 4,104,174 + +0.5 4,104,175 - -1.4 4,104,175 - +0.9 4,104,224 + -0.4 4,104,241 - -1.1 4,104,371 - -0.1
Or see this region's nucleotide sequence