Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3600 and Pf1N1B4_3601 overlap by 4 nucleotides Pf1N1B4_3601 and Pf1N1B4_3602 overlap by 37 nucleotides Pf1N1B4_3602 and Pf1N1B4_3603 are separated by 190 nucleotides
Pf1N1B4_3600 - Flagellar sensor histidine kinase FleS, at 4,011,318 to 4,012,541
_3600
Pf1N1B4_3601 - Flagellar regulatory protein FleQ, at 4,012,538 to 4,013,380
_3601
Pf1N1B4_3602 - Flagellar regulatory protein FleQ, at 4,013,344 to 4,013,931
_3602
Pf1N1B4_3603 - Flagellar hook-basal body complex protein FliE, at 4,014,122 to 4,014,451
_3603
Position (kb)
4012
4013
4014 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4011.629 kb on + strand, within Pf1N1B4_3600 at 4011.758 kb on - strand, within Pf1N1B4_3600 at 4011.882 kb on - strand, within Pf1N1B4_3600 at 4011.936 kb on + strand, within Pf1N1B4_3600 at 4011.937 kb on - strand, within Pf1N1B4_3600 at 4012.191 kb on - strand, within Pf1N1B4_3600 at 4012.281 kb on + strand, within Pf1N1B4_3600 at 4012.281 kb on + strand, within Pf1N1B4_3600 at 4012.281 kb on + strand, within Pf1N1B4_3600 at 4012.282 kb on - strand, within Pf1N1B4_3600 at 4012.282 kb on - strand at 4012.282 kb on - strand, within Pf1N1B4_3600 at 4012.346 kb on - strand, within Pf1N1B4_3600 at 4012.380 kb on - strand, within Pf1N1B4_3600 at 4012.564 kb on - strand at 4012.632 kb on - strand, within Pf1N1B4_3601 at 4012.687 kb on + strand, within Pf1N1B4_3601 at 4012.687 kb on + strand, within Pf1N1B4_3601 at 4012.776 kb on + strand, within Pf1N1B4_3601 at 4012.777 kb on - strand, within Pf1N1B4_3601 at 4012.777 kb on - strand, within Pf1N1B4_3601 at 4012.779 kb on + strand, within Pf1N1B4_3601 at 4012.845 kb on - strand, within Pf1N1B4_3601 at 4013.055 kb on - strand, within Pf1N1B4_3601 at 4013.055 kb on - strand, within Pf1N1B4_3601 at 4013.092 kb on + strand, within Pf1N1B4_3601 at 4013.407 kb on + strand, within Pf1N1B4_3602 at 4013.408 kb on - strand, within Pf1N1B4_3602 at 4013.408 kb on - strand, within Pf1N1B4_3602 at 4013.408 kb on - strand, within Pf1N1B4_3602 at 4013.870 kb on - strand at 4013.914 kb on + strand at 4013.914 kb on + strand at 4013.914 kb on + strand at 4013.915 kb on - strand at 4013.915 kb on - strand at 4013.915 kb on - strand at 4014.099 kb on + strand at 4014.099 kb on + strand at 4014.100 kb on - strand at 4014.100 kb on - strand at 4014.100 kb on - strand at 4014.100 kb on - strand at 4014.100 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 4,011,629 + Pf1N1B4_3600 0.25 +0.4 4,011,758 - Pf1N1B4_3600 0.36 +0.4 4,011,882 - Pf1N1B4_3600 0.46 +0.0 4,011,936 + Pf1N1B4_3600 0.50 +0.4 4,011,937 - Pf1N1B4_3600 0.51 +2.1 4,012,191 - Pf1N1B4_3600 0.71 +2.0 4,012,281 + Pf1N1B4_3600 0.79 +1.0 4,012,281 + Pf1N1B4_3600 0.79 +1.0 4,012,281 + Pf1N1B4_3600 0.79 -1.1 4,012,282 - Pf1N1B4_3600 0.79 +0.5 4,012,282 - -1.1 4,012,282 - Pf1N1B4_3600 0.79 +0.6 4,012,346 - Pf1N1B4_3600 0.84 +0.7 4,012,380 - Pf1N1B4_3600 0.87 +1.2 4,012,564 - -0.4 4,012,632 - Pf1N1B4_3601 0.11 +2.4 4,012,687 + Pf1N1B4_3601 0.18 +0.3 4,012,687 + Pf1N1B4_3601 0.18 +0.8 4,012,776 + Pf1N1B4_3601 0.28 +0.1 4,012,777 - Pf1N1B4_3601 0.28 +0.2 4,012,777 - Pf1N1B4_3601 0.28 +0.9 4,012,779 + Pf1N1B4_3601 0.29 +0.7 4,012,845 - Pf1N1B4_3601 0.36 +1.9 4,013,055 - Pf1N1B4_3601 0.61 -0.4 4,013,055 - Pf1N1B4_3601 0.61 -1.2 4,013,092 + Pf1N1B4_3601 0.66 +0.8 4,013,407 + Pf1N1B4_3602 0.11 +0.2 4,013,408 - Pf1N1B4_3602 0.11 -2.6 4,013,408 - Pf1N1B4_3602 0.11 -1.5 4,013,408 - Pf1N1B4_3602 0.11 -2.8 4,013,870 - -0.3 4,013,914 + +1.4 4,013,914 + -1.8 4,013,914 + +0.5 4,013,915 - -2.1 4,013,915 - -0.3 4,013,915 - -0.5 4,014,099 + -0.1 4,014,099 + +0.2 4,014,100 - -1.8 4,014,100 - +0.2 4,014,100 - -2.8 4,014,100 - +1.2 4,014,100 - -1.1
Or see this region's nucleotide sequence